Diagnosis and treatment of viral diseases

Patent metadata

Field	Value
Publication number	US-9933427-B2
Application number	US-201715439495-A
Country	US
Kind code	B2
Filing date	Feb 22, 2017
Priority date	Jan 8, 2013
Publication date	Apr 3, 2018
Grant date	Apr 3, 2018

Abstract

Provided are methods of diagnosing a viral disease such as idiopathic pulmonary fibrosis, Castleman's disease, a lymphoma, a thymoma or a sarcoma in a patient by identifying one or more virus-specific elements such as a nucleic acid or a viral protein or a patient antibody to a virus-specific element, as well as to kits for diagnosing the viral disease in a patient. Further provided are methods of monitoring disease progression and/or the efficacy of therapy by measuring the levels of a virus-specific element in a sample from a patient, and methods of identifying therapeutic agents that show efficacy in reducing levels of virus-specific agents in vitro. Still further provided are methods of treating idiopathic pulmonary fibrosis, a lymphoproliferative disease and cancer, as well as to methods of preventing viral infection, including Herpesvirus saimiri infection.

First claim

What is claimed is:

1. A method of diagnosing and treating idiopathic pulmonary fibrosis in a human patient comprising the steps of:
(a) providing
(i) a clinical sample of a human patient comprising lung tissue or a bronchial lavage, and
(ii) monoclonal antibodies to at least two Herpesvirus saimiri protein targets selected from the group consisting of viral DHFR, viral cyclin D, viral IL-17 and viral thymidylate synthase, wherein the at least two monoclonal antibodies are specific to said Herpesvirus saimiri protein targets versus their human analogs;

(b) contacting said clinical sample (i) with said monoclonal antibodies (ii);
(c) allowing binding to take place between said monoclonal antibodies (ii) and said protein targets of the antibodies in said clinical sample (i) if present;
(d) detecting binding of said monoclonal antibodies (ii) to said protein targets in said clinical sample (i), thereby providing a diagnosis of said human patient as having idiopathic pulmonary fibrosis; and
(e) after detecting step (d), administering a monoclonal antibody that specifically binds a Herpesvirus saimiri protein to the human patient in treatment of the diagnosed idiopathic pulmonary fibrosis.

2. The method of claim 1 , wherein the monoclonal antibody administered to the human patient is a human monoclonal antibody or a humanized monoclonal antibody.

3. A method of diagnosing and treating idiopathic pulmonary fibrosis in a human subject comprising the steps of:
(a) providing
(i) a clinical sample of a human subject who may have idiopathic pulmonary fibrosis, the clinical sample comprising lung tissue or a bronchial lavage, and
(ii) one or more monoclonal antibodies to viral proteins expressed by Herpesvirus saimiri , wherein the one or more monoclonal antibodies are specific to said Herpesvirus saimiri protein targets versus their human analogs;

(b) contacting said clinical sample (i) with said one or more monoclonal antibodies (ii);
(c) allowing binding to take place between said one or more monoclonal antibodies (ii) and said viral proteins in said clinical sample (i) if present;
(d) detecting the binding of said one or more antibodies (ii) to said viral proteins in the clinical sample (i), thereby providing a diagnosis of said human subject as having idiopathic pulmonary fibrosis; and
(e) after detecting step (d), administering a monoclonal antibody that specifically binds a Herpesvirus saimiri protein to the human patient in treatment of the diagnosed idiopathic pulmonary fibrosis.

4. The method of claim 3 , wherein one of said viral proteins is Herpesvirus saimiri IL-17.

5. The method of claim 3 , wherein one of said viral proteins is Herpesvirus saimiri DHFR.

6. The method of claim 3 , wherein one of said viral proteins is Herpesvirus saimiri cyclin D.

7. The method of claim 3 , wherein one of said viral proteins is Herpesvirus saimiri thymidylate synthase.

8. The method of claim 3 , wherein one of said viral proteins is a viral capsid protein of Herpesvirus saimiri.

9. A method of diagnosing and treating idiopathic pulmonary fibrosis comprising the steps of:
(a) providing
(i) a clinical sample from a human patient suspected of having idiopathic pulmonary fibrosis, wherein the clinical sample comprises lung tissue or bronchial lavage from the human patient, and
(ii) a labeled nucleic acid hybridization probe specific for one or more sequences of Herpesvirus saimiri;

(b) contacting said clinical sample (i) with said labeled nucleic acid probe (ii);
(c) allowing hybridization to take place between said labeled nucleic acid probe (ii) and viral sequences in said clinical sample (i) if present;
(d) detecting hybridization of said nucleic acid hybridization probe (ii) to said one or more sequences in said clinical sample (i), wherein detection of said hybridization provides a diagnosis of said human patient as having idiopathic pulmonary fibrosis; and
(e) based on the diagnosis provided in step (d), administering an antagonist of a Herpesvirus saimiri viral protein to the human patient.

10. The method of claim 9 , wherein said clinical sample comprises a paraffin embedded slide.

11. The method of claim 9 , wherein said detecting step comprises in situ hybridization or flow cytometry.

12. The method of claim 9 , wherein said providing step comprises isolation of nucleic acids from said clinical sample.

13. The method of claim 9 , further comprising a nucleic acid amplification step before said detecting step.

Assignees

• Enzo Biochem Inc
• Enzo Biochem Inc

Inventors

• Nuovo Gerard
• Nivar Virginia

Classifications

• C07K16/244

  Interleukins [IL] · CPC title

• C07K2317/33

  Crossreactivity, e.g. for species or epitope, or lack of said crossreactivity · CPC title

• C07K2317/76

  Antagonist effect on antigen, e.g. neutralization or inhibition of binding · CPC title

• C12Q1/6886

  for cancer (immunoassay for cancer G01N33/575) · CPC title

• C12Q1/705

  for herpetoviridae, e.g. herpes simplex, varicella zoster · CPC title