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Reduction of lipase activity in product formulations
US9932591B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9932591-B2 |
| Application number | US-201415105925-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 18, 2014 |
| Priority date | Dec 18, 2013 |
| Publication date | Apr 3, 2018 |
| Grant date | Apr 3, 2018 |
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- Title
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Abstract
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The invention relates a method for producing a stable recombinant protein, comprising growing a non-naturally occurring host cell in a culture medium to produce a recombinant protein, and making a composition comprising the recombinant protein and a polysorbate. The production of endogenous lipoprotein lipase by the host cell is reduced. The endogenous lipoprotein lipase is present in the composition in a small amount, and is capable of degrading the polysorbate. The invention also relates to the relevant host cells and compositions, and preparation thereof.
First claim
Opening claim text (preview).
What is claimed: 1. A non-naturally occurring host cell comprising a nucleic acid sequence encoding recombinant protein, wherein the host cell expresses the recombinant protein, wherein the host cell expresses an interfering RNA specific for an endogenous lipoprotein lipase, whereby the production of the endogenous lipoprotein lipase by the host cell is reduced. 2. The non-naturally occurring host cell of claim 1 , wherein the host cell is a mammalian cell selected from the group consisting of CHO, 3T3, BHK, HeLa, HepG2, and derivatives thereof. 3. The non-naturally occurring host cell of claim 1 , wherein the interfering RNA is selected from the group consisting of small interfering RNAs (siRNAs), short hairpin RNAs (shRNAs), and bifunctional RNAs. 4. The non-naturally occurring host cell of claim 1 , wherein the interfering RNA is encoded by the genome of the host cell.
Assignees
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Classifications
Glycopeptides, glycoproteins · CPC title
Isomerases (5) · CPC title
Carboxypeptidase C (3.4.16.5), i.e. carboxypeptidase Y · CPC title
having a known sequence of two or more amino acids, e.g. glutathione · CPC title
Oxidoreductases (1) · CPC title
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- What does patent US9932591B2 cover?
- The invention relates a method for producing a stable recombinant protein, comprising growing a non-naturally occurring host cell in a culture medium to produce a recombinant protein, and making a composition comprising the recombinant protein and a polysorbate. The production of endogenous lipoprotein lipase by the host cell is reduced. The endogenous lipoprotein lipase is present in the compo…
- Who is the assignee on this patent?
- Lee Kelvin, Lenhoff Abraham, Valente Kristin, and 3 more
- What technology area does this patent fall under?
- Primary CPC classification C12N15/1137. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 03 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).