Suspension container for binding particles for the isolation of biological material

Patent metadata

Field	Value
Publication number	US-9932574-B2
Application number	US-201715611527-A
Country	US
Kind code	B2
Filing date	Jun 1, 2017
Priority date	Sep 21, 2011
Publication date	Apr 3, 2018
Grant date	Apr 3, 2018

Abstract

A device, method and system is provided for binding particles for the separation and/or isolation of biological materials. In particular, a container is provided including a suspension of binding particles for the isolation of biological material, inner walls forming an elongate groove at the bottom of said container, and a cover having openings and/or being penetrable for a linear arrangement of multiple pipets or pipet tips, said openings being located above and parallel to said elongate groove.

First claim

What is claimed:

1. A suspension container, comprising:
a suspension of binding particles for the isolation of biological material;
inclined inner walls forming an elongate groove at the bottom of said container, said elongate groove being positioned along a straight line at the bottom of the container; and
a cover having openings adapted to receive a linear arrangement of multiple parallel pipets or pipet tips, said openings being located above and parallel to said elongate groove.

2. The suspension container of claim 1 , wherein said binding particles comprise nucleic acid binding particles.

3. The suspension container of claim 1 , wherein said binding particles comprise magnetic glass particles.

4. The suspension container of claim 1 , further comprising one or more elements selected from the group consisting of:
fill-openings for filling the suspension of binding particles into the container; and
a removable foil sealing the cover.

5. The suspension container of claim 1 , wherein said cover has openings, said openings being covered by a penetrable septum.

6. The suspension container of claim 1 , wherein said container is adapted to hold a volume of at least 100 ml.

7. The suspension container of claim 1 , wherein said container comprises one or more fastenings for reversibly fixing the container to a shaker.

8. The suspension container of claim 1 , wherein the footprint of said container is oval-shaped.

9. An analytical system for providing binding particles for the isolation of biological material, said analytical system comprising:
a linear arrangement of multiple parallel pipets or pipet tips;
a suspension container, comprising a suspension of binding particles for the isolation of biological material; inclined inner walls forming an elongate groove at the bottom of said container, said elongate groove being positioned along a straight line at the bottom of the container; and a cover having openings adapted to receive said linear arrangement of multiple parallel pipets or pipet tips, said openings being located above and parallel to said elongate groove; and
a shaker for agitating said container and suspending said binding particles.

10. The analytical system of claim 9 , wherein the system further comprises a plurality of vessels for receiving said suspension of binding particles from said linear arrangement of multiple parallel pipets or pipet tips.

11. The analytical system of claim 9 , said system further comprising a separation module for the isolation of said biological material with said binding particles.

12. The analytical system of claim 9 , said system further comprising one or more elements selected from the group consisting of:
a reaction module containing the components of a chemical and/or biochemical reaction;
a detection module for detecting signals evoked by an analyte;
a storage module for reagents and/or disposables; and
a control unit for controlling system components.

Assignees

• Roche Molecular Systems Inc

Inventors

• Burghardt Geza
• Mulder Willem
• Schneebeli Rolf

Classifications

• B01L2300/0672

  Integrated piercing tool · CPC title

• B03C1/01

  by addition of magnetic adjuvants · CPC title

• B01L2200/141

  Preventing contamination, tampering · CPC title

• B01L7/52

  with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title