Methods of pretreating cellulosic material with a family 61 polypeptide

Patent metadata

Field	Value
Publication number	US-9932414-B2
Application number	US-201113882833-A
Country	US
Kind code	B2
Filing date	Nov 2, 2011
Priority date	Nov 2, 2010
Publication date	Apr 3, 2018
Grant date	Apr 3, 2018

Abstract

The present invention relates to methods of degrading or converting a cellulosic material pretreated with a composition comprising one or more GH61 polypeptides.

First claim

What is claimed is:

1. A method of degrading a cellulosic material, comprising:
(a) pretreating the cellulosic material with a composition comprising one or more Family 61 glycoside hydrolase (GH61) polypeptides; and
(b) saccharifying the GH61 polypeptide pretreated cellulosic material with an enzyme composition,
wherein the pretreating with GH61 polypeptide is performed prior to saccharification.

2. The method of claim 1 , which further comprises treating the cellulosic material with a chemical pretreatment, a physical pretreatment, or a chemical pretreatment and a physical pretreatment.

3. The method of claim 2 , wherein the pretreating with the one or more GH61 polypeptides is performed before, during, or after the chemical pretreatment, the physical pretreatment, or the chemical pretreatment and the physical pretreatment.

4. The method of claim 1 , wherein the one or more GH61 polypeptides are inactivated following pretreatment of the cellulosic material.

5. The method of claim 1 , wherein the enzyme composition of step (b) comprises one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.

6. The method of claim 1 , further comprising recovering the degraded cellulosic material.

7. A method of producing a fermentation product, comprising:
(a) pretreating a cellulosic material with a composition comprising one or more GH61 polypeptides;
(b) saccharifying the GH61 pretreated cellulosic material with an enzyme composition;
(c) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(d) recovering the fermentation product from the fermentation wherein the pretreating with GH61 polypeptide is preformed prior to saccharification.

8. The method of claim 7 , which further comprises treating the cellulosic material with a chemical pretreatment, a physical pretreatment, or a chemical pretreatment and a physical pretreatment.

9. The method of claim 8 , wherein the pretreating with the one or more GH61 polypeptides is performed before, during, or after the chemical pretreatment, the physical pretreatment, or the chemical pretreatment and the physical pretreatment.

10. The method of claim 7 , wherein the one or more GH61 polypeptides are inactivated following pretreatment of the cellulosic material.

11. The method of claim 7 , wherein the enzyme composition comprises one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.

12. A method of producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with an enzyme composition, wherein the cellulosic material is pretreated with a composition comprising one or more GH61 polypeptides;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation wherein the pretreating with GH61 polypeptide is preformed prior to saccharification.

13. The method of claim 12 , which further comprises treating the cellulosic material with a chemical pretreatment, a physical pretreatment, or a chemical pretreatment and a physical pretreatment.

14. The method of claim 13 , wherein the pretreating with the one or more GH61 polypeptides is performed before, during, or after the chemical pretreatment, the physical pretreatment, or the chemical pretreatment and the physical pretreatment.

15. The method of claim 12 , wherein the one or more GH61 polypeptides are inactivated following pretreatment of the cellulosic material.

16. The method of claim 12 , wherein the enzyme composition comprises one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.

17. A method of fermenting a cellulosic material, comprising: fermenting the cellulosic material with one or more fermenting microorganisms, wherein the cellulosic material is pretreated with a composition comprising one or more GH61 polypeptides and is saccharified with an enzyme composition wherein the pretreating with GH61 polypeptide is preformed prior to saccharification.

18. The method of claim 17 , which further comprises treating the cellulosic material with a chemical pretreatment, a physical pretreatment, or a chemical pretreatment and a physical pretreatment.

19. The method of claim 18 , wherein the pretreating with the one or more GH61 polypeptides is performed before, during, or after the chemical pretreatment, the physical pretreatment, or the chemical pretreatment and the physical pretreatment.

20. The method of claim 17 , wherein the one or more GH61 polypeptides are inactivated following pretreatment of the cellulosic material.

21. The method of claim 17 , wherein the enzyme composition comprises one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.

22. The method of claim 17 , wherein the fermenting of the cellulosic material produces a fermentation product.

23. The method of claim 22 , further comprising recovering the fermentation product from the fermentation.

Assignees

• Quinlan Jason
• Xu Feng
• Novozymes Inc

Inventors

• Quinlan Jason
• Xu Feng

Classifications

• C12P7/02

  containing a hydroxy group · CPC title

• C12P19/02 Primary

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12P7/10

  substrate containing cellulosic material · CPC title

• Y02E50/16

  Cross-Sectional Technologies · mapped topic

• C12P5/02

  acyclic {(C12P5/007 takes precedence)} · CPC title