Biological materials related to HER3

Patent metadata

Field	Value
Publication number	US-9932403-B2
Application number	US-201113698992-A
Country	US
Kind code	B2
Filing date	May 20, 2011
Priority date	May 20, 2010
Publication date	Apr 3, 2018
Grant date	Apr 3, 2018

Abstract

The present disclosure relates to amino acid sequences that are directed against (as defined herein) HER3, as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences (also referred to herein as “amino acid sequences of the invention”, “compounds of the invention”, and “polypeptides of the invention”, respectively). The disclosure also relates to nucleic acids encoding such amino acid sequences and polypeptides (also referred to herein as “nucleic acids of the invention” or “nucleotide sequences of the invention”); to methods for 10 preparing such amino acid sequences and polypeptides; to host cells expressing or capable of expressing such amino acid sequences or polypeptides; to compositions, and in particular to pharmaceutical compositions, that comprise such amino acid sequences, polypeptides, nucleic acids and/or host cells; and to uses of such amino acid sequences or polypeptides, nucleic acids, host cells and/or compositions, in particular for prophylactic, therapeutic or diagnostic purposes, such as the prophylactic, therapeutic or diagnostic purposes mentioned herein.

First claim

The invention claimed is:

1. A protein or polypeptide comprising at least two immunoglobulin single variable (ISV) domains that are each directed to and/or that can each specifically bind to human HER3 (SEQ ID NO: 1), in which each such ISV domain is independently:
(a) capable of inhibiting or blocking binding of HRG to HER-3; and/or
(b) capable of inhibiting or blocking heterodimerization of HER-3; and/or
(c) capable of binding to domain II of HER-3,
wherein said protein or polypeptide comprises:
(i) an ISV that is a 17B05-like sequence comprising:
(A) a CDR1 comprising the amino acid sequence LNAMA (SEQ ID NO: 58);
(B) a CDR2 comprising the amino acid sequence GIFGVGSTRYADSVKG (SEQ ID NO: 88); and
(C) a CDR3 comprising the amino acid sequence SSVTRGSSDY (SEQ ID NO: 118); and

(ii) an ISV that is a 21F06-like sequence comprising:
(A) a CDR1 comprising the amino acid sequence LNAMG (SEQ ID NO: 67);
(B) a CDR2 comprising the amino acid sequence AIDWSDGNKDYADSVKG (SEQ ID NO: 97) or AIDWSEGNKDYADSVKG (SEQ ID NO: 445); and
(C) a CDR3 comprising the amino acid sequence DTPPWGPMIYIESYDS (SEQ ID NO: 127) or DTPPWGPLIYIESYDS (SEQ ID NO: 446).

2. The protein or polypeptide according to claim 1 , further functionalized to have an increased half-life, for example by functionalisation and/or by including in the protein or polypeptide a moiety or binding unit that increases the half-life of the construct.

3. The protein or polypeptide according to claim 2 , wherein the functionalization comprises (i) including a moiety or binding unit; and/or (ii) including a peptide or binding unit that can bind to a serum protein.

4. The protein or polypeptide according to claim 1 , comprising an ISV domain directed to human serum albumin.

5. The protein or polypeptide according to claim 1 , selected from the group consisting of SEQ ID NO:282 and SEQ ID NO:199.

6. A pharmaceutical composition comprising a protein or polypeptide or ISV according to claim 1 .

7. The protein or polypeptide according to claim 1 , wherein said 17B05-like sequence is SEQ ID NO: 13, optionally, with one or more of the amino acid residues at positions 11, 37, 44, 47, 83, 84, 103, 104 and 108 according to the Kabat numbering chosen from the Hallmark residues mentioned in the following table:

Position
Human VH 3
Hallmark Residues

11
L, V
L, S, V, M, W, F, T, Q, E, A, R, G, K, Y, N,

P, I

37
V, I, F
F, Y, V, L, A, H, S, IW, C, N, G, D, T, P

48
G
E, Q, G, D, A, K, R, L, P, S, V, H, T, N, W,

M, I

45
L
L, R, P, H, F, G, Q, S, E, T, Y, C, I, D, V

47
W, Y
F, L, W, G, I, S, A, V, M, R, Y, E, P, T, C, H,

K, Q, N, D

83
R, K
R, K, T, E, Q, N, S, I, V, G, M, L, A, D, Y, H

84
A, T, D
P, S, H, L, A, V, I, T F, D, R, Y, N, Q, G, E

103
W
W, R, G, S, K, A, M, Y, L, F, T, N, V, Q, P,

E, C

104
G
G, A, S, T, D, P, N, E, C, L

108
L, M, T
Q, L, R, P, E, K, S, T, M, A, H

wherein when positions 44, 45, 46, and 47 are G,L,E, and W, respectively, postion 108 is always Q in non-humanized V HH sequences that also contain a W at position 103.

8. The protein or polypeptide according to claim 2 , wherein said 21F06-like sequence is SEQ ID NO: 22, optionally, with one or more of the amino acid residues at position 11, 37, 44, 47, 83, 84, 103, 104 and 108 according to the Kabat numbering chosen from the Hallmark residues mentioned in the following table:

Position
Human VH 3
Hallmark Residues

11
L, V
L, S, V, M, W, F, T, Q, E, A, R, G, K, Y, N,

P, I

37
V, I, F
F, Y, V, L, A, H, S, IW, C, N, G, D, T, P

48
G
E, Q, G, D, A, K, R, L, P, S, V, H, T, N, W,

M, I

45
L
L, R, P, H, F, G, Q, S, E, T, Y, C, I, D, V

47
W, Y
F, L, W, G, I, S, A, V, M, R, Y, E, P, T, C, H,

K, Q, N, D

83
R, K
R, K, T, E, Q, N, S, I, V, G, M, L, A, D, Y, H

84
A, T, D
P, S, H, L, A, V, I, T F, D, R, Y, N, Q, G, E

103
W
W, R, G, S, K, A, M, Y, L, F, T, N, V, Q, P,

E, C

104
G
G, A, S, T, D, P, N, E, C, L

108
L, M, T
Q, L, R, P, E, K, S, T, M, A, H

Assignees

• Knuehl Christine
• Hock Bjoern
• Hofmeister Robert
• Beste Gerald
• Revets Hilde Adi Pierrette
• Verdonck Frank Kamiel Delphina
• Cornelis Sigrid Godelieve Victor
• Ablynx Nv

Inventors

• Knuehl Christine
• Hock Bjoern
• Hofmeister Robert
• Beste Gerald
• Revets Hilde Adi Pierrette
• Verdonck Frank Kamiel Delphina
• Cornelis Sigrid Godelieve Victor

Classifications

• C07K2317/77

  Internalization into the cell · CPC title

• C07K16/2863 Primary

  against receptors for growth factors, growth regulators · CPC title

• C07K16/18

  against material from animals or humans · CPC title

• C07K2317/76

  Antagonist effect on antigen, e.g. neutralization or inhibition of binding · CPC title

• C07K2317/73

  Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation · CPC title