Cold- and menthol-sensitive receptor (CMR1)

Patent metadata

Field	Value
Publication number	US-9932383-B2
Application number	US-201615266016-A
Country	US
Kind code	B2
Filing date	Sep 15, 2016
Priority date	Jan 25, 2002
Publication date	Apr 3, 2018
Grant date	Apr 3, 2018

Abstract

The present invention relates to regulation of cold sensation and pain. More particularly, the present invention is directed to nucleic acids encoding a member of the transient regulatory protein family, CMR1, which is involved in modulation of the perception of cold sensations and pain. In some embodiments, an isolated or recombinant CMR1 polypeptide is directly or indirectly attached to a detectable label or fused to a second polypeptide. In some embodiments, an isolated or recombinant CMR1 polypeptide is directly or indirectly bound to a solid support.

First claim

What is claimed is:

1. An isolated or recombinant cold- and menthol-sensitive receptor (CMR1) polypeptide comprising SEQ ID NO:3 directly or indirectly attached to a detectable label or fused to a second polypeptide.

2. The isolated or recombinant polypeptide of claim 1 , wherein the CMR1 polypeptide is fused to a second polypeptide.

3. The isolated or recombinant polypeptide of claim 1 , wherein the label is selected from 32 P, fluorescent dyes, electron-dense reagents, biotin, digoxygenin, haptens, and radiolabeled proteins.

4. An isolated membrane comprising the isolated or recombinant polypeptide of claim 1 .

5. An isolated membrane comprising the isolated or recombinant polypeptide of claim 2 .

6. An isolated membrane comprising the isolated or recombinant polypeptide of claim 3 .

7. An isolated or recombinant cold- and menthol-sensitive receptor (CMR1) polypeptide comprising SEQ ID NO:3 directly or indirectly bound to a solid support.

8. The isolated or recombinant polypeptide of claim 7 , wherein the CMR1 polypeptide is bound covalently to the solid support.

9. The isolated or recombinant polypeptide of claim 7 , wherein the CMR1 polypeptide is bound non-covalently to the solid support.

10. The isolated or recombinant polypeptide of claim 7 , wherein the solid support comprises a nitrocellulose filter, a nylon filter, or a derivatized nylon filter.

Assignees

• Univ California

Inventors

• Julius David
• Mckemy David D
• Neuhausser Werner M

Classifications

• G01N33/5041

  involving analysis of members of signalling pathways · CPC title

• C07K2319/00

  Fusion polypeptide · CPC title

• C12N15/00

  Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor (mutants or genetically engineered microorganisms, per se C12N1/00, C12N5/00, C12N7/00; new plants per se A01H; plant reproduction by tissue culture techniques A01H4/00; new animals per se A01K67/00; use of medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases, gene therapy A61K48/00) · CPC title

• C07K14/705 Primary

  Receptors; Cell surface antigens; Cell surface determinants {(tumour specific antigens C07K14/4748)} · CPC title

• G01N2333/705

  Assays involving receptors, cell surface antigens or cell surface determinants · CPC title