Method of measuring food dyes in bodily waste to identify and quantify drug consumption

Patent metadata

Field	Value
Publication number	US-9927448-B1
Application number	US-201715465939-A
Country	US
Kind code	B1
Filing date	Mar 22, 2017
Priority date	Mar 22, 2017
Publication date	Mar 27, 2018
Grant date	Mar 27, 2018

Abstract

We disclose a drug tracking system and method of use which may be used to screen a subject's bodily waste and to identify the drug the subject has consumed. The system includes a drug that is tagged with a food dye that may be detected in the subject's bodily waste using absorption spectroscopic analysis. The subject consumes the tagged drug and a user obtains a sample of the subject's bodily waste. The user may analyze the subject's urine using an absorption spectroscopic technique. The user may enter the emission spectrum obtained from the absorption spectroscopic analysis into a database that includes the signature emission spectra from multiple food dyes that are used as drug tags. The emission spectra may be compared and the identity of the food dye and its associated drug may be determined.

First claim

We claim:

1. A method of tracking drug consumption comprising the steps of:
obtaining a urine sample from a subject who has consumed a drug, wherein the drug has been tagged with a food dye, wherein the food dye or a reaction product of the food dye is detectable by an absorption spectroscopic analysis of the urine sample; wherein the food dye is exclusively used to tag a single defined drug;
conducting a first absorption spectroscopic analysis of the urine sample to detect the food dye or the reaction product of the food dye;
conducting at least one follow-up absorption spectroscopic analysis of the urine sample, wherein the at least one follow-up spectroscopic analysis comprises the step of exposing the urine sample to a single excitation wavelength, wherein the single excitation wavelength is selected based on a wavelength of a peak in an emission spectrum that is produced by the first absorption spectroscopic analysis of the urine sample, wherein the first absorption spectroscopic analysis comprises the step of exposing the urine sample to a first energy source, and wherein the first energy source emits a range of excitation wavelengths.

2. The method of claim 1 , wherein the food dye consists of green number 3, wherein the single excitation wavelength is about 625 nm.

3. The method of claim 1 , wherein the food dye consists of red number 3, wherein the single excitation wavelength is about 530 nm.

4. The method of claim 1 , wherein the food dye consists of methylene blue, wherein the single excitation wavelength is about 668 nm.

5. The method of claim 1 , wherein the food dye consists of indigo carmine, wherein the single excitation wavelength is about 609 nm.

6. The method of claim 1 , wherein the food dye consists of cochineal carmine red, wherein the single excitation wavelength is about 513 nm.

7. The method of claim 1 , wherein the food dye consists of tartrazine, wherein the single excitation wavelength is about 425 nm.

8. The method of claim 1 , wherein the reaction product of the food dye consists of leucomethylene blue, wherein the single excitation wavelength is about 246 nm.

9. The method of claim 1 , wherein the food dye comprises indigo carmine, and further comprising the step of adjusting a pH value of the urine sample to an optimal level for measuring a peak absorption wavelength of the food dye.

10. A method of tracking drug consumption comprising the steps of:
obtaining a urine sample from a subject who has consumed a drug, wherein the drug has been tagged with a food dye, wherein the food dye or a reaction product of the food dye is detectable by an absorption spectroscopic analysis of the urine sample; wherein the food dye is exclusively used to tag a single defined drug;
conducting a first absorption spectroscopic analysis of the urine sample to detect the food dye or the reaction product of the food dye;
measuring a concentration of a urine metabolite in the urine sample; and
normalizing a height of a peak within an emission spectrum with the concentration of the urine metabolite in the urine sample, wherein emission spectrum is produced by the first absorption spectroscopic analysis of the urine sample.

11. A method of tracking drug consumption comprising the steps of:
obtaining a urine sample from a subject who has consumed a drug, wherein the drug has been tagged with a food dye, wherein the food dye or a reaction product of the food dye is detectable by an absorption spectroscopic analysis of the urine sample; wherein the food dye is exclusively used to tag a single defined drug;
conducting a first absorption spectroscopic analysis of the urine sample to detect the food dye or the reaction product of the food dye;
measuring specific gravity of the urine sample; and
normalizing a height of a peak within an emission spectrum with a measurement of specific gravity of the urine sample, wherein the emission spectrum is derived from the first absorption spectroscopic analysis of the urine sample.

Assignees

• Hall David R
• Butala Steven
• Allen Dan
• Hendricks Daniel
• Nguyen Andrew
• Pearman Terrece

Inventors

• Hall David R
• Butala Steven
• Allen Dan
• Hendricks Daniel
• Nguyen Andrew
• Pearman Terrece

Classifications

• G01N33/583

  with non-fluorescent dye label · CPC title

• G01N33/493

  urine · CPC title

• G01N21/25

  Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands · CPC title

• Y10T436/13

  Tracers or tags · CPC title

• G01N33/94 Primary

  involving narcotics {or drugs or pharmaceuticals, neurotransmitters or associated receptors} · CPC title