Nozzle assembly for a flow cytometer

Patent metadata

Field	Value
Publication number	US-9927345-B2
Application number	US-201615204838-A
Country	US
Kind code	B2
Filing date	Jul 7, 2016
Priority date	Sep 19, 2012
Publication date	Mar 27, 2018
Grant date	Mar 27, 2018

Abstract

A nozzle assembly is disclosed. The nozzle assembly may include a nozzle outlet and enclose a nozzle cavity. A sample inlet formed in the nozzle housing may provide fluid communication to an injection tube mounted in an injection stem formed in the nozzle housing. One or more sheath inlet may provide fluid communication with the nozzle cavity and a downstream exit orifice.

First claim

We claim:

1. A nozzle assembly comprising:
a nozzle housing comprising a nozzle cap and a nozzle base that enclose a nozzle cavity, the nozzle housing further comprising an injection stem formed in the nozzle cap that protrudes into the nozzle cavity;
a sample inlet formed in the nozzle cap of the nozzle housing;
an injection tube having a sample outlet, the injection tube being mounted with the injection stem and extending along the nozzle cavity, wherein the injection tube comprises a flow path providing fluid communication between the sample inlet formed in the nozzle cap of the nozzle housing and the sample outlet of the injection tube;
one or more sheath inlets in fluid communication with the nozzle cavity; and
a nozzle exit orifice downstream of the sample outlet.

2. The nozzle assembly of claim 1 , wherein the one or more sheath inlets are formed in the nozzle cap.

3. The nozzle assembly of claim 1 , wherein the injection stem further comprises a pocket and wherein the injection tube is mounted with the pocket.

4. The nozzle assembly of claim 1 , wherein the nozzle housing is injection molded with the injection tube.

5. The nozzle assembly of claim 1 , wherein nozzle housing is over molded onto the injection tube.

6. The nozzle assembly of claim 1 , wherein the injection tube comprises a ceramic injection tube.

7. The nozzle assembly of claim 6 , wherein the ceramic injection tube is beveled at the sample outlet.

8. The nozzle assembly of claim 6 , wherein the flow path is at least partially defined by an interior geometry of the ceramic injection tube.

9. The nozzle assembly of claim 8 , wherein the interior geometry of the ceramic injection tube is tapered inwardly towards the sample outlet.

10. The nozzle assembly of claim 8 , wherein the internal geometry of the ceramic injection tube at, or towards, the sample outlet is selected from the group consisting of: an oval, a square, a trapezoid, a rectangle, a cone, an inward taper, an outward tape, and combinations thereof.

11. The nozzle assembly of claim 1 , further comprising an orienting nozzle tip, wherein the nozzle tip is keyed such that the nozzle tip fits with the nozzle housing in a specified orientation.

12. The nozzle assembly of claim 1 , further comprising a seating element mounted with the nozzle housing.

13. The nozzle assembly of claim 12 , wherein the seating element comprises a material selected from a group consisting of: a metal, a magnetic material, an electromagnetic material, and combinations thereof.

14. The nozzle assembly of claim 12 , wherein the seating element is injection molded with the nozzle housing.

15. The nozzle assembly of claim 12 , wherein the seating element comprises a nozzle clamp.

16. The nozzle assembly of claim 15 , wherein the nozzle clamp comprises one or more threaded portions for receiving one or more fasteners to secure two or more portions of the nozzle housing.

17. The nozzle assembly of claim 15 , wherein the seating element is keyed to ensure the nozzle housing is mounted in a specified position or in a specified orientation.

18. A flow cytometer comprising the nozzle assembly of claim 1 .

Assignees

• Inguran Llc

Inventors

• Buchanan Kris
• Sharpe Johnathan Charles
• Evans Kenneth Michael

Classifications

• G01N15/14

  Optical investigation techniques, e.g. flow cytometry · CPC title

• Y10T29/49401

  Fluid pattern dispersing device making, e.g., ink jet · CPC title

• G01N15/1404 Primary

  Handling flow, e.g. hydrodynamic focusing · CPC title

• G01N33/487

  of liquid biological material · CPC title

• G01N15/1434

  Optical arrangements · CPC title