Encapsulating device and encapsulating method for encapsulating a sample in a polymer capsule

Patent metadata

Field	Value
Publication number	US-9926551-B2
Application number	US-201414902072-A
Country	US
Kind code	B2
Filing date	Jun 27, 2014
Priority date	Jul 26, 2013
Publication date	Mar 27, 2018
Grant date	Mar 27, 2018

Abstract

The invention relates to an encapsulating device ( 100 ), which is designed to encapsulate a sample ( 1, 2 ) in a polymer capsule, comprising a drop generator ( 10 ), which is designed to provide a drop ( 3 ) of a suspension, which drop contains the sample ( 1 ), and a cross-linking device ( 20 ), which is designed to polymerize the drop ( 3 ), wherein the drop generator ( 10 ) has a retaining device ( 11 ), which is designed to accommodate the drop ( 3 ) in a hanging state, and the cross-linking device ( 20 ) is designed to feed a polymerization substance to the hanging drop ( 3 ) on the retaining device ( 11 ) and to form the polymer capsule. The invention further relates to a method for encapsulating a sample ( 1, 2 ) in a polymer capsule.

First claim

The invention claimed is:

1. An encapsulation device, which is arranged to encapsulate a sample in a polymer capsule, comprising:
a drop generator, which is arranged to provide a drop of a suspension that contains the sample, the drop generator comprising a holding device arranged to receive the drop of the suspension and hold the drop of the suspension in a hanging state to form a hanging suspension drop; and
a cross-linking device arranged to supply a polymerization substance to the hanging suspension drop on the holding device and to form the polymer capsule.

2. The encapsulation device according to claim 1 , in which the holding device comprises at least one of
a holding plate having an exposed underside, which is arranged to receive the hanging drop,
a hole plate, which has a hole for receiving the hanging drop,
a mesh plate, which is arranged to receive the hanging drop, and
a holding rod, which is arranged to receive the hanging drop.

3. The encapsulation device according to claim 1 , in which
the drop generator has a feed device, which is arranged to feed the suspension to the holding device and to form the hanging drop on the holding device.

4. The encapsulation device according to claim 3 , in which
the feed device comprises a first pipetting device, which is arranged to place the hanging drop on the holding device.

5. The encapsulation device according to claim 1 , in which the cross-linking device comprises at least one of
a particle shooting device, which is arranged to inject the polymerization substance into the hanging drop,
a second pipetting device, which is arranged to introduce the polymerization substance into the hanging drop, and
a bowl for receiving the polymerization substance, the holding device and the bowl being disposed so as to be movable relative to each other, such that the hanging drop can be immersed in the polymerization substance in the bowl.

6. The encapsulation device according to claim 1 , in which
a covering device is provided, which is arranged to cover the polymer capsule with a covering substance.

7. The encapsulation device according to claim 6 , in which
the covering device comprises at least one fluid line, which is fixedly connected to the holding device.

8. The encapsulation device according to claim 1 , further comprising
a measuring device, which is arranged to examine the hanging drop and/or the polymer capsule.

9. The encapsulation device according to claim 1 , further comprising
a separating device, which is arranged to separate the polymer capsule from the holding device.

10. A method for encapsulating a sample in a polymer capsule, comprising the steps:
providing an encapsulation device according to claim 1 ,
providing by use of the drop generator a drop of a suspension that contains the sample, and
polymerizing the drop using the cross-linking device, wherein
the drop is provided in a hanging state on the holding device, and
the polymerizing comprises supplying of a polymerization substance to the hanging drop on the holding device and a polymerization reaction in the drop, such that the polymer capsule is formed.

11. The method according to claim 10 , in which the providing of the hanging drop comprises at least one of
receiving the hanging drop on an exposed underside of a holding plate,
receiving the hanging drop in the holes of a hole plate,
receiving the hanging drop on an exposed underside of a mesh plate, and
receiving the hanging drop on a holding rod.

12. The method according to claim 10 , in which
the providing of the hanging drop comprises placing the hanging drop on the holding device by use of a first pipetting device.

13. The method according to claim 10 , in which the polymerizing comprises at least one of
introducing the polymerization substance into the hanging drop by use of a second pipetting device,
injecting the polymerization substance into the hanging drop by use of a particle shooting device, and
immersing the hanging drop in the polymerization substance in a bowl.

14. The method according to claim 10 , further comprising at least one of the steps
covering the polymer capsule with a covering substance, and
examining the hanging drop or the polymer capsule.

15. The method according to claim 10 , further comprising the step
separating the polymer capsule from the holding device.

16. The method according to claim 10 , in which
the suspension contains an alginate solution, and the polymerization substance contains cross-linking ions by way of which the alginate solution is polymerized in the hanging drop, or
the suspension contains cross-linking ions, and the polymerization contains an alginate solution, which is polymerized in the hanging drop.

17. The method according to claim 10 , in which
the sample contains at least one of at least one biological cell, at least one cell component and at least one cell group.

18. The encapsulation device according to claim 1 , in which the holding device comprises
a receptacle for the hanging suspension drop so that a top side of the hanging suspension drop can adhere to the holding device.

Assignees

• Fraunhofer Ges Forschung
• Fraunhofer Ges Forschung

Inventors

• Zimmermann Heiko
• Neubauer Julia

Classifications

• C12N11/04 Primary

  entrapped within the carrier, e.g. gel or hollow fibres · CPC title

• B01J13/14 Primary

  Polymerisation; cross-linking · CPC title

• C12N11/10

  the carrier being a carbohydrate · CPC title

• C12M25/01

  Drops · CPC title

• A61K2035/128

  capsules, e.g. microcapsules · CPC title