Micro blood vessels and tissue ducts

Patent metadata

Field	Value
Publication number	US-9926534-B2
Application number	US-201514789440-A
Country	US
Kind code	B2
Filing date	Jul 1, 2015
Priority date	Jun 9, 2005
Publication date	Mar 27, 2018
Grant date	Mar 27, 2018

Abstract

A fiber includes one or more layers of polymer surrounding a central lumen, and living animal cells disposed within the lumen and/or within at least one of the one or more layers, wherein the fiber has an outer diameter of between 5 and 8000 microns and wherein each individual layer of polymer has a thickness of between 0.1 and 250 microns. Also disclosed are model tissues including such fibers, and method of making such fibers. The fibers can serve as synthetic blood vessels, ducts, or nerves.

First claim

What is claimed is:

1. A method of generating a tubular fiber, the method comprising:
providing a channel having a proximal end and a distal end, said channel having opposed facing top and bottom surfaces, said channel having at least one first fluid transporting structure across said channel located on said top surface and at least one second fluid transporting structure across said channel located on said bottom surface, said first and second fluid transporting structures being located between said proximal and said distal end and on said opposing surfaces, facing one another across the channel;
creating a sheath flow comprising a core stream surrounded by one or more sheath streams by introducing the sheath stream and the core stream into the proximal end of the channel and receiving the sheath flow from the distal end thereof, wherein at least one of the core or sheath streams comprises a polymerizable material and wherein at least one of the core or sheath streams comprises living animal cells; and
polymerizing the polymerizable material thereby forming a tubular a fiber
wherein the fiber comprises:
one or more layers of polymer derived from the one or more sheath streams surrounding a central lumen derived from the core stream, and
the living animal cells disposed within the lumen and/or within at least one of the one or more layers,
wherein the fiber has an outer diameter of between 5 and 8000 microns and wherein each individual layer of polymer has a thickness of between 0.1 and 250 microns.

2. The method of claim 1 , wherein the fiber is configured as a blood vessel, tissue duct, or nerve.

3. The method of claim 1 , wherein the fiber further comprises cells adhered to an exterior surface of said fiber.

4. The method of claim 1 , wherein the fiber is free of attachment along a surface.

5. The method of claim 1 , wherein the polymer in at least one of said one or more layers is biodegradable.

6. The method of claim 1 , wherein the polymer in at least one of said one or more layers comprises a material is selected from the group consisting of collagen, agarose, polyelectrolytes, chitosan, gelatin, polyethylene glycol, peptides, and combinations thereof.

7. The model tissue of claim 1 , wherein at least one of said one or more layers further comprises a nucleic acid and/or a factor to modify cell growth, adhesion, and/or differentiation.

8. A method of generating a tubular fiber, the method comprising:
providing a channel having a proximal end and a distal end, said channel having opposed facing top and bottom surfaces, said channel having at least one first fluid transporting structure across said channel located on said top surface and at least one second fluid transporting structure across said channel located on said bottom surface, said first and second fluid transporting structures being located between said proximal and said distal end and on said opposing surfaces, facing one another across the channel;
creating a sheath flow comprising a core stream surrounded by two or more concentric sheath streams by introducing the core stream and two or more source sheath streams into the proximal end of the channel and receiving the sheath flow from the distal end thereof, wherein at least one of the core or sheath streams comprises a polymerizable material and wherein at least one of the core or sheath streams comprises living animal cells; and
polymerizing the polymerizable material thereby forming a tubular a fiber
wherein the fiber comprises:
one or more layers of polymer derived from the two or more concentric sheath streams surrounding a central lumen derived from the core stream, and
the living animal cells disposed within the lumen and/or within at least one of the one or more layers, wherein at least two different animal cells are disposed in different layers of polymer and/or lumen
wherein the fiber has an outer diameter of between 5 and 8000 microns and wherein each individual layer of polymer has a thickness of between 0.1 and 250 microns.

9. The method of claim 8 , wherein the fiber is configured as a blood vessel, tissue duct, or nerve.

10. The method of claim 8 , wherein the fiber further comprises cells adhered to an exterior surface of said fiber.

11. The method of claim 8 , wherein the fiber is free of attachment along a surface.

12. The method of claim 8 , wherein the polymer in at least one of said one or more layers is biodegradable.

13. The method of claim 8 , wherein the polymer in at least one of said one or more layers comprises a material is selected from the group consisting of collagen, agarose, polyelectrolytes, chitosan, gelatin, polyethylene glycol, peptides, and combinations thereof.

Assignees

• Adams Andre A
• Daniele Michael
• Ligler Frances S
• Us Navy

Inventors

• Adams André A
• Daniele Michael
• Ligler Frances S

Classifications

• B01L2400/0487

  fluid pressure, pneumatics · CPC title

• B01J2219/00891

  Feeding or evacuation · CPC title

• B01L2300/0838

  Capillaries · CPC title

• C12M25/14

  Scaffolds; Matrices (in general C12N5/0068) · CPC title

• C12M21/08

  for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00) · CPC title