Methods for engineering sugar transporter preferences

Patent metadata

Field	Value
Publication number	US-9926347-B2
Application number	US-201415034772-A
Country	US
Kind code	B2
Filing date	Nov 5, 2014
Priority date	Nov 5, 2013
Publication date	Mar 27, 2018
Grant date	Mar 27, 2018

Abstract

Provided herein are compositions and methods useful for transporting xylose, arabinose and other monosaccharides, into a yeast cell.

First claim

What is claimed is:

1. A non-naturally occurring, recombinant xylose transporter protein comprising a transporter motif sequence corresponding to amino acid residue positions 36, 37, 38, 39, 40, and 41 of SEQ ID NO: 1 of Candida intermedia GXS1 protein, wherein said transporter motif sequence is mutated to -G-G/F-X 1 -X 2 -X 3 -G-(SEQ ID NO: 29);
wherein,
X 1 is D, C, G, H, I, L, or F;
X 2 is A, D, C, E, G, H, or I;
X 3 is N, C, Q, F, G, L, M, S, T, or P; and
wherein, said transporter motif sequence is not -G-G-L-I-F-G- (SEQ ID NO: 2) or -G-G-F-I-F-G-(SEQ ID NO: 3);
and further wherein said transporter motif sequence allows transport of xylose into a cell.

2. The non-naturally occurring, recombinant xylose transporter protein of claim 1 , wherein X 1 is F, X 2 is I, and X 3 is M or S.

3. The non-naturally occurring, recombinant xylose transporter protein of claim 1 , wherein said transporter motif sequence is -G-G-F-I-M-G-(SEQ ID NO: 4), -G-F-F-I-M-G-(SEQ ID NO: 5), -G-G-F-I-S-G-(SEQ ID NO: 6), -G-F-F-I-S-G-(SEQ ID NO: 7), -G-G-F-I-T-G-(SEQ ID NO: 8), -G-F-F-I-T-G-(SEQ ID NO: 9), -G-G-F-L-M-G-(SEQ ID NO: 10), -G-F-F-L-M-G-(SEQ ID NO: 11), -G-G-F-L-S-G-(SEQ ID NO: 12), -G-F-F-L-S-G-(SEQ ID NO: 13), -G-G-F-L-T-G-(SEQ ID NO: 14), -G-F-F-L-T-G-(SEQ ID NO: 15), -G-G-F-H-M-G-(SEQ ID NO: 16), -G-F-F-H-M-G-(SEQ ID NO: 17), -G-G-F-H-S-G-(SEQ ID NO: 18), -G-F-F-H-S-G-(SEQ ID NO: 19), -G-G-F-H-T-G-(SEQ ID NO: 20) or -G-F-F-H-T-G-(SEQ ID NO: 21).

4. The non-naturally occurring, recombinant xylose transporter protein of claim 3 further comprising a mutation of an amino acid at the residue position corresponding to 297 of Candida intermedia GXS1 protein.

5. The non-naturally occurring, recombinant xylose transporter protein of claim 4 , wherein said amino acid at the residue position corresponding to 297 of Candida intermedia GXS1 protein is substituted with a Met, Ala, Ser, or Asn residue.

Assignees

• Univ Texas

Inventors

• Alper Hal
• Young Eric

Classifications

• Y02E50/16

  Cross-Sectional Technologies · mapped topic

• C12P7/10

  substrate containing cellulosic material · CPC title

• C07K14/40 Primary

  from Candida · CPC title

• C07K14/39

  from yeasts · CPC title

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title