Methods of producing and using silk microfibers

Patent metadata

Field	Value
Publication number	US-9925301-B2
Application number	US-201314390362-A
Country	US
Kind code	B2
Filing date	Apr 5, 2013
Priority date	Apr 6, 2012
Publication date	Mar 27, 2018
Grant date	Mar 27, 2018

Abstract

Provided herein relates to methods for preparing micron range silk fibers (or silk microfibers) and compositions comprising a micron range silk fiber (or a silk microfiber). The micron range silk fibers (or silk microfibers) can be used in various applications ranging from fillers in cosmetics to reinforcement materials to design high strength composites, e.g., reinforced scaffolds. In some embodiments, the silk microfiber-reinforced scaffolds can be used for bone graft applications because of their high compressive strength.

First claim

What is claimed is:

1. A method comprising
contacting a plurality of degummed silk fibers with an alkaline solution under conditions in which the degummed silk fibers undergo alkaline hydrolysis to provide silk microfibers having an aspect ratio of length to width of at least 2:1, and
reinforcing a matrix material by associating at least some of the silk microfibers with the matrix material to form a composite material.

2. The method of claim 1 , wherein the length of the silk microfibers ranges from about 1 μm to about 2 mm.

3. The method of claim 1 , wherein the alkaline solution comprises sodium hydroxide, potassium hydroxide, lithium hydroxide, rubidium hydroxide, cesium hydroxide, calcium hydroxide, strontium hydroxide, barium hydroxide, or any combinations thereof.

4. The method of claim 1 , wherein the alkaline solution has a concentration of about 0.1 M to about 30 M.

5. The method of claim 1 , wherein the contacting is maintained for a duration ranging from about 5 seconds to about 5 hours.

6. The method of claim 1 , wherein the contacting is performed at about 4° C. to about 100° C.

7. The method of claim 1 , further comprising neutralizing the mixture comprising one or more silk microfibers and the alkaline solution.

8. The method of claim 1 , further comprising annealing the silk microfibers.

9. The method of claim 1 , further comprising separating a subset of the silk microfibers of a desired length from the plurality of silk microfibers.

10. The method of claim 1 , wherein the matrix material is polymeric material, ceramic material or combination thereof.

11. The method of claim 10 , wherein the polymeric material is selected from the group consisting of polyethylene oxide, polyethylene glycol, collagen, fibronectin, keratin, silk, silk fibroin, polyaspartic acid, polylysine, alginate, chitosan, chitin, hyaluronic acid, pectin, polycaprolactone, polylactic acid, polyglycolic acid, polyhydroxyalkanoates, dextrans, polyanhydrides, poly(lactide-co-glycolide) (PLA-PLA-PGA), polymethylmethacrylate, polyanhydride, polyorthoester, polycaprolactone, polyfumarate, chitosan, alginate, and any combination thereof.

12. The method of claim 10 , wherein the ceramic material is selected from the group consisting of calcium phosphate, calcium sulfate, hydroxyapatite, bioactive glass, and any combination thereof.

13. The method of claim 1 , wherein the composite material is porous.

14. The method of claim 1 , wherein the composite material is non-porous.

Assignees

• Tufts College

Inventors

• Kaplan David L
• Mandal Biman B

Classifications

• A61L27/58

  Materials at least partially resorbable by the body · CPC title

• A61L27/56

  Porous materials, {e.g. foams or sponges} · CPC title

• A61L27/3821

  Bone-forming cells, e.g. osteoblasts, osteocytes, osteoprogenitor cells · CPC title

• C08K7/02

  Fibres or whiskers · CPC title

• D06M11/36

  with oxides, hydroxides or mixed oxides; with salts derived from anions with an amphoteric element-oxygen bond · CPC title