Drive control method for objective lens and fluorescence microscope system

Patent metadata

Field	Value
Publication number	US-9921398-B2
Application number	US-201414915210-A
Country	US
Kind code	B2
Filing date	Aug 25, 2014
Priority date	Aug 27, 2013
Publication date	Mar 20, 2018
Grant date	Mar 20, 2018

Abstract

Provided is a driving control method of an objective lens in which an optical-axis chromatic aberration can be corrected by driving the objective lens, and an image can be quickly captured by realizing a quick driving and stabilizing of the objective lens so as to acquire a three-dimensional image at a high speed. A driving control method of the objective lens driven by a piezoelectric actuator provided in a fluorescence microscope includes a first step of applying a pulse voltage larger than a displacement voltage making the objective lens move to a focal position of an observation target to the piezoelectric actuator for a predetermined time so as to move the objective lens near the focal position, and a second step of applying the displacement voltage to the piezoelectric actuator after the first step to stabilize the objective lens.

First claim

The invention claimed is:

1. A driving control method of an objective lens, provided in a fluorescence microscope, to correct an optical-axis chromatic aberration caused by the objective lens which is driven by a piezoelectric actuator, the method comprising:
a first step of applying a pulse voltage to the piezoelectric actuator for a predetermined time so as to move the objective lens near a focal position of an observation target, the pulse voltage being larger than a displacement voltage needed to move the objective lens to the focal position; and
a second step of applying the displacement voltage to the piezoelectric actuator after the first step to stabilize the objective lens.

2. The driving control method of the objective lens according to claim 1 , wherein a multiplied voltage of the displacement voltage is applied as the pulse voltage in the first step.

3. A fluorescence microscope system comprising:
a light source;
an objective lens that introduces a light beam emitted from the light source to an observation target;
a piezoelectric actuator that drives the objective lens in an optical axis direction; and
a driving unit that first applies a pulse voltage to the piezoelectric actuator for a predetermined time to move the objective lens near a focal position of the observation target, the pulse voltage being larger than a displacement voltage needed to move the objective lens to the focal position, and then applies the displacement voltage to the piezoelectric actuator so as to stabilize the objective lens.

4. The fluorescence microscope system according to claim 3 , wherein the driving unit applies a multiplied voltage of the displacement voltage as the pulse voltage.

Assignees

• Riken

Inventors

• Nakano Akihiko
• Ichihara Akira

Classifications

• G02B27/0025

  for optical correction, e.g. distorsion, aberration · CPC title

• G02B21/02

  Objectives · CPC title

• G02B21/16 Primary

  adapted for ultraviolet illumination {; Fluorescence microscopes (G02B21/0076 takes precedence)} · CPC title

• G02B21/08

  Condensers · CPC title

• G02B27/0068

  having means for controlling the degree of correction, e.g. using phase modulators, movable elements (controlling the phase of light using moving or deformable elements G02B26/06) · CPC title