Maize cytoplasmic male sterility (CMS) S-type restorer gene Rf3

Patent metadata

Field	Value
Publication number	US-9920331-B2
Application number	US-201414586135-A
Country	US
Kind code	B2
Filing date	Dec 30, 2014
Priority date	Dec 31, 2013
Publication date	Mar 20, 2018
Grant date	Mar 20, 2018

Abstract

A newly identified protein that is encoded by a polynucleotide sequence associated with cytoplasmic male sterility restorer activity (Rf3) is described. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to restore cytoplasmic male sterility in plants. Further applications of the newly identified polynucleotide sequence associated with cytoplasmic male sterility restorer activity include a mutation (rf3) which results in cytoplasmic male sterility. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to result in cytoplasmic male sterility in plants. Methods for detecting the cytoplasmic male sterility restorer (Rf3) and the cytoplasmic male sterility (rf3) gene sequences are further described.

First claim

What is claimed is:

1. A fusion polypeptide comprising an amino acid sequence having at least 97.5% sequence to the amino acid sequence of SEQ ID NO: 3, wherein said fusion polypeptide comprises an amino acid motif comprising the amino acid sequence of SEQ ID NO: 1; and wherein said fusion polypeptide comprises cytoplasmic male sterile activity.

2. The fusion polypeptide of claim 1 , wherein said fusion polypeptide is comprised by a cell.

3. The fusion polypeptide of claim 1 , wherein said fusion polypeptide is encoded by a synthetic nucleic acid sequence.

4. The fusion polypeptide of claim 3 , wherein said synthetic nucleic acid sequence is operably linked to a promoter.

5. The fusion polypeptide of claim 4 , wherein said promoter is functional in plants.

6. The fusion polypeptide of claim 5 , wherein said promoter is a cytoplasmic male sterile gene promoter.

Assignees

• Dow Agrosciences Llc

Inventors

• Ren Ruihua
• Nagel Bruce A
• Ye Liang
• Gao Yanxin Star
• Gibson Ryan
• Paulraj Sushmitha
• Mansfield Tyler
• Mammadov Jafar
• Kumpatla Siva P
• Thompson Steven A

Classifications

• C12N15/8289

  Male sterility · CPC title

• C07K14/415

  from plants · CPC title

• C12N15/8287 Primary

  for fertility modification, e.g. apomixis · CPC title

• A01H1/02

  Methods or apparatus for hybridisation; Artificial pollination {; Fertility} · CPC title

• A01H1/023

  Male sterility · CPC title