Sequence determined DNA fragments and corresponding polypeptides encoded thereby

Patent metadata

Field	Value
Publication number	US-9920328-B2
Application number	US-201213629871-A
Country	US
Kind code	B2
Filing date	Sep 28, 2012
Priority date	Sep 30, 2003
Publication date	Mar 20, 2018
Grant date	Mar 20, 2018

Abstract

The present invention provides DNA molecules that constitute fragments of the genome of a plant, and polypeptides encoded thereby. The DNA molecules are useful for specifying a gene product in cells, either as a promoter or as a protein coding sequence or as an UTR or as a 3′ termination sequence, and are also useful in controlling the behavior of a gene in the chromosome, in controlling the expression of a gene or as tools for genetic mapping, recognizing or isolating identical or related DNA fragments, or identification of a particular individual organism, or for clustering of a group of organisms with a common trait.

First claim

What is claimed is:

1. A recombinant nucleic acid comprising:
a nucleic acid molecule having a nucleotide sequence which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:261, 733 or having at least 95% amino acid sequence identity to the amino acid sequence of SEQ ID NO:261, 733;
wherein the polypeptide encodes a Dof zinc finger protein;
wherein the nucleic acid molecule is operably linked to a heterologous regulatory sequence.

2. The recombinant nucleic acid according to claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO:261, 732.

3. The recombinant nucleic acid according to claim 1 , wherein the nucleic acid molecule encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:261, 733.

4. A vector construct comprising the recombinant nucleic acid of claim 1 .

5. A host cell comprising the recombinant nucleic acid molecule according to claim 1 .

6. A host cell comprising a vector construct according to claim 4 .

7. A method of introducing recombinant nucleic acid into a host cell comprising:
a) providing the recombinant nucleic acid according to claim 1 ; and
b) contacting said recombinant nucleic acid with said host cell under conditions that permit insertion of said recombinant nucleic acid into said host cell.

8. A method of transforming a host cell which comprises contacting the host cell with the vector construct according to claim 4 .

9. A plant, plant cell, plant material or seed of a plant which comprises the recombinant nucleic acid according to claim 1 .

10. A plant, plant cell, plant material or seed of a plant which comprises a vector construct according to claim 4 , wherein the plant, plant cell, plant material or seed comprises the recombinant nucleic acid.

11. A plant that has been regenerated from a plant cell or seed according to claim 9 or 10 , wherein the plant comprises the recombinant nucleic acid.

12. Transgenic progeny of the plant of claim 9 or 10 , wherein the progeny comprises the recombinant nucleic acid.

13. Seed from the plant according to claim 9 , wherein the seed comprises the recombinant nucleic acid.

Assignees

• Ceres Inc

Inventors

• Alexandrov Nickolai
• Brover Vyacheslav
• Feldmann Kenneth A
• Makarov Vladimir
• Swaller Timothy J
• Nadzan Gregory
• Mascia Peter
• Troukhan Maxim
• Rarang Joel
• Burns James
• Vos Strache Kyle

Classifications

• C07K14/415

  from plants · CPC title

• C12N15/8261 Primary

  with agronomic (input) traits, e.g. crop yield · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12Q2600/13

  Plant traits · CPC title