Root-preferred promoter from a brachypodium distachyon metallothionein-like gene

Patent metadata

Field	Value
Publication number	US-9914933-B2
Application number	US-201615097345-A
Country	US
Kind code	B2
Filing date	Apr 13, 2016
Priority date	Apr 15, 2015
Publication date	Mar 13, 2018
Grant date	Mar 13, 2018

Abstract

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Brachypodium distachyon metallothionein-like gene (mtl). Some embodiments relate to a promoter from a Brachypodium distachyon metallothionein-like gene (mtl) that functions in plants to promote transcription of operably linked nucleotide sequences.

First claim

What is claimed is:

1. A nucleic acid vector comprising a promoter operably linked to:
a) a polylinker sequence;
b) a non metallothionein-like heterologous coding sequence; or
c) a combination of a) and b);

wherein said promoter comprises a polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:1, and said promoter has below ground tissue preferred expression.

2. The nucleic acid vector of claim 1 , wherein said promoter is 2.0 Kb in length.

3. The nucleic acid vector of claim 1 , wherein said promoter consists of a polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:1.

4. The nucleic acid vector of any one of claims 1 - 3 , further comprising a sequence encoding a selectable maker.

5. The nucleic acid vector of claim 1 , wherein said promoter is operably linked to a heterologous coding sequence.

6. The nucleic acid vector of claim 5 , wherein the heterologous coding sequence encodes a selectable marker, an insecticidal resistance protein, a small RNA molecule, a site specific nuclease protein, a herbicide tolerance protein, a nitrogen use efficiency protein, a water use efficiency protein, a nutritional quality protein or a DNA binding protein.

7. The nucleic acid vector of any of claims 1 - 3 , or 5 , further comprising a 3′ untranslated polynucleotide sequence comprising SEQ ID NO:3, wherein the 3′ untranslated sequence is operably linked to said polylinker or said heterologous coding sequence.

8. The nucleic acid vector of any of claims 1 - 3 or 5 , further comprising a 5′ untranslated sequence.

9. The nucleic acid vector of any of claims 1 - 3 or 5 , further comprising an intron sequence.

10. A non- Brachypodium plant comprising a polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:1 operably linked to a heterologous coding sequence, wherein said heterologous coding sequence has below ground tissue preferred expression.

11. The plant of claim 10 , wherein said plant is selected from the group consisting of maize, wheat, rice, sorghum , oats, rye, bananas, sugar cane, soybean, cotton, Arabidopsis , tobacco, sunflower, and canola.

12. The plant of claim 10 , wherein said plant is maize.

13. The plant of any one of claims 10 - 12 , wherein the heterologous coding sequence is inserted into the genome of said plant.

14. The plant of claim 10 , further comprising a 3′ untranslated sequence comprising SEQ ID NO:3, wherein the 3′ untranslated sequence is operably linked to said heterologous coding sequence.

15. The plant of claim 10 , wherein said promoter is 2.0 Kb in length.

Assignees

• Dow Agrosciences Llc

Inventors

• Kumar Sandeep
• Hemingway Daren
• Ausmus Carla
• Worden Andrew F
• Asberry Andrew

Classifications

• C12N15/8227 Primary

  Root-specific · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/8216

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• C07K14/825

  Metallothioneins · CPC title