Methods and compositions for reducing the proliferation of gram positive bacteria

Patent metadata

Field	Value
Publication number	US-9913854-B2
Application number	US-201314388685-A
Country	US
Kind code	B2
Filing date	Mar 14, 2013
Priority date	Mar 28, 2012
Publication date	Mar 13, 2018
Grant date	Mar 13, 2018

Abstract

Provided herein are methods for inducing cell death in a gram positive bacterium and methods for reducing the proliferation of a gram positive bacterium that include contacting a gram positive bacterium with a dye (e.g., an azo dye) and a TarO inhibitor. Also provided are methods of treating a subject having a gram positive bacterial infection that include administering to a subject having a gram positive bacterial infection a dye (e.g., an azo dye) and a TarO inhibitor. Also provided are compositions containing a dye (e.g., an azo dye) and a TarO inhibitor, and kits containing at least one of these compositions.

First claim

The invention claimed is:

1. A method for inducing cell death in a gram positive bacterium or reducing the proliferation of a gram positive bacterium, the method comprising contacting a gram positive bacterium with Congo Red and tunicamycin in amounts sufficient to induce cell death in the gram positive bacterium or amounts sufficient to reduce the proliferation of the gram positive bacterium.

2. The method of claim 1 , wherein the gram positive bacterium is present in a mammal.

3. The method of claim 1 , wherein the gram positive bacterium is present in vitro.

4. The method of claim 3 , wherein the gram positive bacterium is present in a cell culture, is present in or on a food composition, or is present in a pharmaceutical or cosmetic composition.

5. The method of claim 1 , wherein the gram positive bacterium is a coccus gram positive bacterium.

6. The method of claim 5 , wherein the coccus gram positive bacterium is from the Streptococcus, Enterococcus , or Staphylococcus genus.

7. The method of claim 1 , wherein the gram positive bacterium is a bacillus gram positive bacterium.

8. The method of claim 7 , wherein the bacillus gram positive bacterium is from the Cornebacterium, Listeria, Bacillus , or Clostridium genus.

9. A method of treating a subject having a gram positive bacterial infection, the method comprising administering to the subject having a gram positive bacterial infection Congo Red and tunicamycin in amounts sufficient to decrease the population of gram positive bacteria in the subject.

10. The method of claim 9 , wherein the population of gram positive bacteria comprises coccus gram positive bacteria.

11. The method of claim 10 , wherein the coccus gram positive bacteria are from the Streptococcus, Enterococcus , or Staphylococcus genus.

12. The method of claim 9 , wherein the population of gram positive bacteria comprises bacillus gram positive bacteria.

13. The method of claim 12 , wherein the bacillus gram positive bacteria are from the Cornebacterium, Listeria, Bacillus , or Clostridium genus.

14. The method of claim 1 , wherein the concentration of Congo Red is less than 1024 mg/L.

15. The method of claim 9 , wherein the amount of the tunicamycin is not toxic to the subject.

Assignees

• Massachusetts Eye & Ear Infirmary

Inventors

• Gilmore Michael S
• Suzuki Takashi

Classifications

• C09B31/043

  Amino-benzenes · CPC title

• C09B33/16

  from other coupling components · CPC title

• C09B67/0097

  Dye preparations of special physical nature; Tablets, films, extrusion, microcapsules, sheets, pads, bags with dyes · CPC title

• A61K31/655

  Azo (—N=N—), diazo (=N2), azoxy (>N—O—N< or N(=O)—N<), azido (—N3) or diazoamino (—N=N—N<) compounds · CPC title

• C07D405/14

  containing three or more hetero rings · CPC title