Antimicrobial compositions comprising glyceryl nitrates

Patent metadata

Field	Value
Publication number	US-9913820-B2
Application number	US-201615260238-A
Country	US
Kind code	B2
Filing date	Sep 8, 2016
Priority date	Aug 8, 2012
Publication date	Mar 13, 2018
Grant date	Mar 13, 2018

Abstract

Provided are antimicrobial solutions comprising a glyceryl nitrate (e.g., glyeryl trinitrate) in combination with a chelator (e.g., citrate), a peroxide, a fatty acid, and/or an alcohol (e.g., ethanol). In various aspects these components may synergistically act to kill or reduce the growth of microbes, such as bacteria or fungi, present in a biofilm.

First claim

The invention claimed is:

1. An antimicrobial solution comprising a glyceryl nitrate and ethanol at a level from about 10%-50% (v/v), and a chelator, wherein the chelator is citrate at an amount of 0.1-10% (v/v); and wherein the solution is a pharmaceutically acceptable composition.

2. The solution of claim 1 , wherein the glyceryl nitrate is glyceryl trinitrate (GTN).

3. The solution of claim 2 , wherein the glyceryl trinitrate has a concentration of from about 0.05 to about 1500 micrograms/ml.

4. The solution of claim 3 , wherein the glyceryl trinitrate has a concentration of from about 1 to about 1000 micrograms/ml.

5. The solution of claim 4 , wherein the glyceryl trinitrate has a concentration of from about 10 to about 500 micrograms/ml.

6. The solution of claim 1 , wherein the citrate comprises about 1-10% (v/v) of the solution.

7. The solution of claim 6 , wherein the citrate comprises about 3.5-7% of the solution.

8. The solution of claim 7 , wherein the citrate comprises about 4% of the solution.

9. The solution of claim 1 , wherein the concentration of the ethanol is in the range of from greater than 10 to about 40%.

10. The solution of claim 9 , wherein the concentration of ethanol is in the range of from about 15-30% (v/v).

11. The solution of claim 1 , wherein the concentration of the ethanol is about 20%-30%.

12. The solution of claim 1 , wherein the solution comprises from greater than 10% to about 30% ethanol, about 1-20% citrate, and about 10-500 microgram/ml glyceryl trinitrate.

13. The solution of claim 1 , wherein the solution comprises from about 15% to about 30% ethanol, about 2.5-10% citrate, and about 50-250 microgram/ml glyceryl trinitrate.

14. A method for reducing microbial organisms from a surface comprising contacting the surface with an antimicrobial solution of claim 1 for an amount of time sufficient to reduce microbial organisms on the surface.

15. The method of claim 14 , wherein the microbial organisms are present in a biofilm on the surface.

16. The method of claim 14 , wherein the surface is comprised on a catheter.

17. The method of claim 16 , wherein the surface is comprised on the interior of a vascular catheter.

18. A kit comprising the solution of claim 1 in a suitable container means.

19. The kit of claim 18 , further comprising instructions for use.

20. The kit of claim 18 , wherein the suitable container means is a vial, syringe or dispenser.

21. The kit of claim 18 , wherein the solution is comprised in or on a swab or wipe.

22. A method of treating a wound in a subject comprising administering or contacting the solution of claim 1 to at least a portion of the wound.

23. The method of claim 22 , wherein the subject is a human.

24. The method of claim 22 , wherein the solution is topically administered to the subject.

25. The method of claim 22 , wherein the solution is administered as a lavage.

26. The method of claim 22 , further comprising irrigating the wound with the solution.

27. The method of claim 22 , wherein the solution is applied to a wound bed, an epithelial tissue, an endothelial tissue, or an organ surface.

28. The method of claim 22 , wherein the solution is used a locking solution in a catheter.

29. The method of claim 11 , wherein the concentration of the ethanol is about 20%-25%.

30. The method of claim 20 , wherein the suitable container means is a syringe.

31. The kit of claim 18 , wherein the solution in is comprised in a cream or ointment.

32. The solution of claim 1 , wherein the solution is further defined as a catheter locking solution.

33. The solution of claim 1 , wherein the glyceryl nitrate is glyceryl trinitrate, and wherein the solution comprises about 15%-25% ethanol, about 2.5-10% citrate, and about 1-100 micrograms/ml glyceryl trinitrate.

34. The solution of claim 33 , wherein the solution comprises about 2.5-7% citrate.

35. The solution of claim 34 , wherein the solution comprises about 10-50 micrograms/ml glyceryl trinitrate.

36. The solution of claim 34 , wherein the solution comprises about 20-40 micrograms/ml glyceryl trinitrate.

37. The solution of claim 36 , wherein the solution comprises about 30 micrograms/ml glyceryl trinitrate.

38. The solution of claim 36 , wherein the solution comprises about 20-25% ethanol.

39. The solution of claim 38 , wherein the solution comprises about 20-22.5% ethanol.

40. The solution of claim 36 , wherein the solution comprises about 3-6% citrate.

41. The solution of claim 40 , wherein the solution comprises about 3-5% citrate.

42. The solution of claim 41 , wherein the solution comprises about 4% citrate.

43. The solution of claim 33 , wherein the solution comprises about 20-22.5% ethanol, about 4% citrate, and about 30 micrograms/ml glyceryl trinitrate.

Assignees

• Univ Texas
• Board Of Regents The Univ Of Texas Systems

Inventors

• Raad Issam
• Rosenblatt Joel

Classifications

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P31/00

  Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics · CPC title

• A61P31/04

  Antibacterial agents · CPC title

• A61P31/02

  Local antiseptics · CPC title

• A61P17/02

  for treating wounds, ulcers, burns, scars, keloids, or the like · CPC title