Polyvalent functionalized nanoparticle-based in vivo diagnostic system

Patent metadata

Field	Value
Publication number	US-9910035-B1
Application number	US-201414333155-A
Country	US
Kind code	B1
Filing date	Jul 16, 2014
Priority date	Jul 16, 2014
Publication date	Mar 6, 2018
Grant date	Mar 6, 2018

Abstract

A system for modulating a response signal includes polyvalent functionalized nanoparticles configured to bind with target analytes located on a surface of a cell, a detector configured to detect an analyte response signal transmitted from the body, a modulation source configured to modulate the analyte response signal, and a processor configured to non-invasively detect the one or more target analytes by differentiating the analyte response signal from a background signal, at least in part, based on the modulation. The analyte response signal is related to the binding interaction of the target analytes on the cell surface with the polyvalent functionalized nanoparticles. In some examples, the system may also include magnetic particles and a magnetic field source sufficient to distribute the magnetic particles into a spatial arrangement in the body. The analyte response signal may be differentiated from the background signal, at least in part, based on modulation of the signals due, at least in part, to the spatial arrangement of the magnetic particles.

First claim

What is claimed is:

1. A system comprising:
polyvalent functionalized nanoparticles for binding with one or more target analytes on a surface of a cell present in an environment;
a detector for detecting:
an analyte response signal transmitted from the environment, wherein the analyte response signal is related to binding of the one or more target analytes on the cell surface with the polyvalent functionalized nanoparticles; and
an unbound polyvalent functionalized nanoparticle signal transmitted from the environment related to polyvalent functionalized nanoparticles that are not interacting with the one or more target analytes;

a modulation source for modulating the analyte response signal and the unbound nanoparticle signal, such that the analyte response signal is affected differently than the unbound nanoparticle signal; and
a processor for non-invasively detecting the one or more target analytes on the cell surface by differentiating the analyte response signal from the unbound nanoparticle signal, based, at least in part, on the modulation,
wherein the modulation source alters one or more properties of the polyvalent functionalized nanoparticles that are bound to the one or more target analytes on the cell surface and wherein the one or more properties of the polyvalent functionalized nanoparticles comprises one or more of: optical properties, magnetic properties, electric properties, thermal properties, acoustic properties, chemical properties, and physical properties.

2. The system of claim 1 , wherein the environment is a living body and further comprising a wearable device having a mount for mounting the wearable device to an external surface of the living body, wherein the detector is mounted on the wearable device.

3. The system of claim 2 , wherein at least a portion of the modulation source is mounted on said wearable device.

4. The system of claim 1 , wherein the processor further differentiates the analyte response signal from a background signal and wherein said modulation source modulates the analyte response signal and the background signal, such that the analyte response signal is affected differently than the background signal, or to alter the analyte response signal by spatially modulating the polyvalent functionalized nanoparticles that bind with the one or more target analytes on the cell surface.

5. The system of claim 1 , wherein the modulation source alters the analyte response signal by modulating an interrogation signal directed into the body.

6. The system of claim 1 , further comprising an interrogating signal source, the analyte response signal being transmitted in response to the interrogating signal.

7. The system of claim 1 , wherein the environment comprises a fluid conduit or a fluid reservoir.

8. The system of claim 1 , wherein the environment is a living body, wherein the polyvalent functionalized nanoparticles are introduced into the living body to bind with one or more target analytes present on a surface of a cell in a body fluid contained in the living body, and wherein the body fluid comprises blood, tear fluid, urine, lymph, cerebrospinal fluid, stool, or mucus.

9. The system of claim 1 , wherein the environment is a living body, wherein the polyvalent functionalized nanoparticles are embedded in skin or tissue of the living body, and wherein the functionalized nanoparticles are introduced into the living body to bind with one or more target analytes present on the surface of a cell in the skin or tissue of the living body.

10. A system, comprising:
polyvalent functionalized nanoparticles for binding with one or more target analytes on a surface of a cell present in an environment;
a detector for detecting a response signal transmitted from the environment, wherein the response signal includes a background signal and an analyte response signal related to binding of the one or more target analytes with polyvalent functionalized nanoparticles;
magnetic particles;
a magnetic field source sufficient to distribute the magnetic particles into a spatial arrangement in the environment, wherein the spatial arrangement of the magnetic particles modulates the analyte response signal, such that the analyte response signal is affected differently than the background signal; and
a processor for non-invasively detecting the one or more target analytes by differentiating the analyte response signal from the background signal based, at least in part, on modulation of the analyte response signal, wherein the analyte response signal is modulated differently than the background signal or modulated differently than the unbound particle signal.

11. The system of claim 10 , wherein the response signal further includes an unbound particle signal related to polyvalent functionalized nanoparticles that are not bound with the one or more target analytes.

12. The system of claim 11 , wherein the processor further non-invasively detects the one or more target analytes by differentiating the analyte response signal from the unbound particle signal, at least in part, based on modulation of the signals due, at least in part, to the spatial arrangement of the magnetic particles.

13. The system of claim 10 , further comprising an interrogating signal source, the response signal being transmitted in response to the interrogating signal.

14. The system of claim 10 , wherein the environment comprises a fluid conduit or fluid reservoir.

15. The system of claim 13 , wherein the environment comprises a living body.

16. The system of claim 15 , wherein the environment comprises a lumen of subsurface vasculature in the living body.

17. A method comprising:
introducing polyvalent functionalized nanoparticles into an environment for binding with one or more target analytes on a surface of a cell present in the environment;
detecting an analyte response signal transmitted from the environment, wherein the analyte response signal is related to binding of the one or more target analytes with the polyvalent functionalized nanoparticles and wherein the response signal is modulated;
detecting an unbound polyvalent functionalized nanoparticle signal transmitted from the environment, wherein the unbound nanoparticle signal is related to polyvalent functionalized nanoparticles that are not interacting with the one or more target analytes;
detecting a background signal;
applying a modulation to a portion of the environment for altering the analyte response signal and the unbound nanoparticle signal, such that the analyte response signal is affected differently than the unbound nanoparticle signal and the background signal; and
differentiating the analyte response signal from the unbound nanoparticles signal and the background signal.

18. The method of claim 17 , further comprising:
directing an interrogating signal into the environment; and
detecting the analyte response signal transmitted from the environment in response to the interrogating signal.

19. The method of claim 17 , wherein the environment comprises a fluid conduit, a fluid reservoir, or a living body.

20. The method of claim 19 , wherein the polyvalent functionalized nanoparticles interacts with one or more target analytes present in the living body.

21. The method of claim 20 , wherein the polyvalent functionalized nanoparticles are embedded in skin or tissue of the living body.

22. The method of claim 21 , wherein the polyvalent functionalized nanoparticles interacts with one or more target analytes pre

Assignees

• Verily Life Sciences Llc

Inventors

• Schwartz Jerrod Joseph
• Audeh Mark
• Bajaj Vikram Singh

Classifications

• G01N33/56966

  Animal cells · CPC title

• A61B5/14507 Primary

  specially adapted for measuring characteristics of body fluids other than blood (devices for taking samples of body liquids A61B10/0045) · CPC title

• A61B5/145 Primary

  Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value {; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue} (non-radiation detecting or locating of foreign bodies in blood A61B5/06) · CPC title

• G01N33/54346 Primary

  Nanoparticles · CPC title

• A61K49/0002

  General or multifunctional contrast agents, e.g. chelated agents · CPC title