Nucleic acid preparation method

Patent metadata

Field	Value
Publication number	US-9909165-B2
Application number	US-201715431678-A
Country	US
Kind code	B2
Filing date	Feb 13, 2017
Priority date	Nov 26, 2010
Publication date	Mar 6, 2018
Grant date	Mar 6, 2018

Abstract

A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support while maintaining it on the support. The polyol may for example be a C 2 -C 10 alkanediol.

First claim

The invention claimed is:

1. A composition comprising:
(i) a polyol or a liquid comprising a polyol, and
(ii) a nucleic acid, wherein the nucleic acid is not dissolved in the polyol or the liquid comprising the polyol, wherein the polyol is 2-methyl-1,3-propanediol, 2,3-butanediol, or tripropylene glycol.

2. The composition of claim 1 , wherein the nucleic acid is bound to a solid support.

3. A kit comprising a polyol or a liquid comprising a polyol, wherein the polyol is 2-methyl-1,3-propanediol, 2,3-butanediol, or tripropylene glycol, at least one of a lysis buffer or a binding buffer, and a solid support.

4. The composition of claim 1 or the kit of claim 3 , wherein the solid support is a bead comprising a silica surface.

5. The composition or kit of claim 4 , wherein the solid support is a magnetic bead.

6. A method of washing a solid support, comprising:
a. providing a solid support, wherein a nucleic acid is bound to the solid support;
b. contacting the solid support with a liquid comprising a polyol, wherein the polyol is 2-methyl-1,3-propanediol, 2,3-butanediol, or tripropylene glycol; and
c. separating the liquid and the solid support.

7. A method of washing a solid support, comprising:
a. contacting a biological sample comprising a nucleic acid with the solid support, whereby the nucleic acid binds to the solid support;
b. contacting the solid support with a liquid comprising a polyol, wherein the polyol is 2-methyl-1,3-propanediol, 2,3-butanediol, or tripropylene glycol; and
c. separating the liquid and the solid support.

8. The method of claim 7 , further comprising washing the solid support with an alcohol free wash buffer.

Assignees

• Life Tech As
• Life Technologies Corp

Inventors

• Ellis Darren
• Fonnum Geir
• Kjus Nini

Classifications

• C12Q1/6806 Primary

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• C12N15/1013

  by using magnetic beads · CPC title

• C12N15/1006

  by means of a solid support carrier, e.g. particles, polymers · CPC title