Microfluidic separation device, separation method using the same and kit for separating circulating rare cells from blood using the same

Patent metadata

Field	Value
Publication number	US-9908117-B2
Application number	US-201514861677-A
Country	US
Kind code	B2
Filing date	Sep 22, 2015
Priority date	Sep 22, 2014
Publication date	Mar 6, 2018
Grant date	Mar 6, 2018

Abstract

The present invention relates to a microfluidic separation device, a separation method using the same and a kit for separating circulating rare cells from blood using the same, and more particularly, to a microfluidic-based separation technology for fixing target particles of a sample, which have a specific affinity for magnetic nanoparticles, to a device by use of a magnetic material, and for isolating the sample from which the target particles have been removed. The present invention may be effectively applied to remove leukocytes from a blood sample in order to isolate circulating rare cells (CRCs), particularly circulating tumor cells (CTCs).

First claim

What is claimed is:

1. A microfluidic separation device for forming a magnetic nanoparticle (MNP) complex comprising target particles bound to MNPs and for capturing and separating the MNP complex using a magnetic force, the microfluidic separation device comprising:
a mixing channel, an incubation channel and a separation channel, which are sequentially arranged to communicate with each other,
wherein the mixing channel comprises:
at least one inlet provided upstream of the mixing channel; and
a curved channel which is wave-shaped, communicates with the at least one inlet, and has two or more curved portions,
wherein the incubation channel comprises a first residence region having an enlarged cross-sectional area over a portion of the incubation channel, and
wherein the separation channel comprises:
an outlet provided downstream of the separation channel;
a plurality of second residence regions which are connected to each other in series and have an enlarged cross-sectional area over a portion of the separation channel to ensure a predetermined residence time and residence space of the MNP complex; and
a plurality of magnetic materials provided on top and bottom outer surfaces of the second residence regions.

2. The microfluidic separation device of claim 1 , wherein the mixing channel further comprises at least one expanded channel which is formed in the curved channel and which have an expanded width over a portion of the at least one expanded channel.

3. The microfluidic separation device of claim 2 , wherein in the mixing channel, a ratio of a width of the curved channel to a width of the expanded channel is 1:6-1:7.

4. The microfluidic separation device of claim 2 , wherein the at least one expanded channel formed in the curved channel includes six to eight expanded channels.

5. The microfluidic separation device of claim 2 , wherein the at least one expanded channel is formed in the curved channel at a position at which one of the curved portions is shifted to a next one of the curved portions.

6. The microfluidic separation device of claim 1 , wherein the at least one inlet of the mixing channel comprises a magnetic nanoparticle inlet and a sample inlet.

7. The microfluidic separation device of claim 1 , wherein a ratio of a cross-sectional area of the first residence region of the incubation channel to across-sectional area of the curved channel of the mixing channel is 1000:1-1500:1.

Assignees

• Univ Yonsei Iacf

Inventors

• Jung Hyo Il
• Hyun Kyung A
• Lee Tae Yoon

Classifications

• B01L3/5085

  for multiple samples, e.g. microtitration plates · CPC title

• G01N33/5434

  using magnetic particle immunoreagent carriers which constitute new materials per se · CPC title

• G01N33/5002

  Partitioning blood components · CPC title

• B01L3/502761 Primary

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• B01F5/0655

  Operations & Transport · mapped topic