Method for isolating microorganisms on a culture medium, and related device

Patent metadata

Field	Value
Publication number	US-9902988-B2
Application number	US-201314415930-A
Country	US
Kind code	B2
Filing date	Jul 22, 2013
Priority date	Jul 20, 2012
Publication date	Feb 27, 2018
Grant date	Feb 27, 2018

Abstract

Method for isolating microorganism from a sample likely contaminated by microorganism, including: (a) device for isolating microorganisms including a bottom waterproof layer, a nutritional layer, which is placed on the bottom layer and includes a dehydrated culture medium, an isolation layer which is pervious to elements included in the nutritional layer and is capable of retaining the bacteria on the surface and covering all or part of the nutritional layer, and a top protective layer; (b) depositing a volume of the sample on the isolation layer; (c) isolating the microorganisms by impoverishing or layering the sample using an isolating device; (d) incubating the device for an amount of time at a temperature to enable growth of microorganisms, method including at least one step of rehydrating the culture medium using a volume of liquid before or with step b) and/or c) and/or d), before or simultaneously with step b) and/or c).

First claim

The invention claimed is:

1. A method for isolating at least one microorganism from a sample, comprising:
(a) supplying a device for isolation of at least one microorganism, the device comprising
a bottom layer impermeable to water;
a nutrient layer, arranged on the bottom layer, comprising a support impregnated with a calendered, dehydrated culture medium;
an isolating layer permeable to the elements comprised in the nutrient layer, able to retain the at least one microorganism on its surface and covering the whole or a portion of the nutrient layer; and
a protective top layer;

(b) depositing a defined volume of the sample on the isolating layer;
(c) isolating the at least one microorganism by exhaustion or by coating the sample using isolating means;
(d) incubating the device for a predetermined time and at a predetermined temperature allowing growth of the at least one microorganism; and
(e) rehydrating the culture medium with a predetermined volume of liquid before or simultaneously with one or more of step (b), step (c), and step (d).

2. The method as claimed in claim 1 , wherein the isolating layer has hydrophilic and/or hydrophobic units.

3. The method as claimed in claim 1 , wherein the device also comprises an identification code.

4. The method as claimed in claim 3 , wherein the identification code is a barcode or a radio-frequency identification (RFID) tag.

5. The method as claimed in claim 1 , wherein the liquid used for rehydrating the culture medium is an aqueous solution and/or a liquid containing microparticles with lipid envelopes.

6. The method as claimed in claim 1 , wherein at least the nutrient layer and the isolating layer are mechanically independent of one another within said device.

Assignees

• Biomerieux Sa
• Biomerieux Sa

Inventors

• Flandrois Jean Pierre
• Limon Bernard
• Rozand Christine
• Montet Marie Pierre

Classifications

• C12M47/04

  Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04) · CPC title

• B32B37/18

  involving the assembly of discrete sheets or panels only · CPC title

• C12M25/02 Primary

  Membranes; Filters (filters or filtration in general B01D24/00-B01D41/00) · CPC title

• C12Q1/24 Primary

  Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms · CPC title

• B01L2300/0825

  Test strips · CPC title