What technology area does this patent fall under?

Primary CPC classification C12N9/2414. Mapped technology areas include Chemistry & Metallurgy.

When was this patent published?

Publication date Tue Feb 27 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.

What related patents are in patentsdb?

We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).

Alpha-amylase variants and polynucleotides encoding same

US9902946B2 · US · B2

Patent metadata
Field	Value
Publication number	US-9902946-B2
Application number	US-201314759068-A
Country	US
Kind code	B2
Filing date	Dec 20, 2013
Priority date	Jan 3, 2013
Publication date	Feb 27, 2018
Grant date	Feb 27, 2018

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Title
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Abstract
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Assignees and inventors
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Key dates
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First independent claim
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CPC / IPC classifications
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Abstract

Official abstract text for this publication.

The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

First claim

Opening claim text (preview).

The invention claimed is: 1. An alpha-amylase variant comprising an alteration at two or more positions corresponding to positions W140, W159, W167, Q169, W189, E194, N260, F262, F289, W284, G304, G305, W347, R439, W469, D476 and G477 of the mature polypeptide of SEQ ID NO: 1, wherein each alteration is independently a substitution, deletion or insertion, and wherein the variant has at least 90%, but less than 100% sequence identity with the mature polypeptide of SEQ ID NO: 1 and wherein the variant has alpha-amylase activity. 2. The variant of claim 1 , comprising an alteration at three or more of said positions. 3. The variant of claim 1 comprising an alteration at four or more of said positions. 4. The variant of claim 1 comprising an alteration at five or more of said positions. 5. The variant of claim 1 comprising an alteration at six or more of said positions. 6. The variant of claim 1 comprising an alteration at seven or more of said positions. 7. The variant of claim 1 comprising an alteration at eight or more of said positions. 8. The variant of claim 1 comprising an alteration at nine or more of said positions. 9. The variant of claim 1 wherein the alterations are substitutions. 10. The variant of claim 9 , wherein the two or more alterations comprise a substitution of W140ACDEFGHIKLMNPQRSTVY. 11. The variant of claim 9 , wherein the two or more alterations comprise a substitution of W159ACDEFGHIKLMNPQRSTVY. 12. The variant of claim 9 , wherein the two or more alterations comprise a substitution of W167ACDEFGHIKLMNPQRSTVY. 13. The variant of claim 9 , wherein the two or more alterations comprise a substitution of Q169ACDEFGHIKLMNPWRSTVY. 14. The variant of claim 9 , wherein the two or more alterations comprise a substitution of W189ACDEFGHIKLMNPQRSTVY. 15. The variant of claim 9 , wherein the two or more alterations comprise a substitution of E194ACDWFGHIKLMNPQRSTVY. 16. The variant of claim 9 , wherein the two or more alterations comprise a substitution of N260ACFGHIKLMWPQRSTVY. 17. The variant of claim 9 , wherein the two or more alterations comprise a substitution of F262ACDEWGHIKLMNPQRSTVY. 18. The variant of claim 9 , wherein the two or more alterations comprise a substitution of W284ACDEFGHIKLMNPQRSTVY. 19. The variant of claim 9 , wherein the two or more alterations comprise a substitution of F289ACDEWGHIKLMNPQRSTVY. 20. The variant of claim 9 , wherein the two or more alterations comprise a substitution of G304ACEFHIKLMNPQRVVTVY. 21. The variant of claim 9 , wherein the two or more alterations comprise a substitution of G305ACEFWHIKLMNPQRTVY. 22. The variant of claim 9 , wherein the two or more alterations comprise a substitution of W347ACDEFGHIKLMNPQRSTVY. 23. The variant of claim 9 , wherein the two or more alterations comprise a substitution of R439ACDEFGHIKLMNPQSTVY. 24. The variant of claim 9 , wherein the two or more alterations comprise a substitution of W469ACDEFGHIKLMNPQRSTVY. 25. The variant of claim 9 , wherein the two or more alterations comprise a substitution of D476ACEFHIKLMNPQRTVY. 26. The variant of claim 9 , wherein the two or more alterations comprise a substitution of G477ACEFHIKLMNPQRTVWY. 27. The variant of claim 9 , further comprising a deletion in two positions selected from the group consisting of R181, G182, D183 and G184 when using SEQ ID NO: 1 for numbering, preferably a deletion of R181+D183, or of R181+184, or of R181+G182, or of G182+D183, or of G182+G184, or more preferred of D183 +G184. 28. The variant of claim 9 , further comprising at least one of the substitutions selected from the group consisting of: G109A, G149A, K179L, G186A, E190P, N195F, M202LIT, V206YF, N209D, Y243F, S244QAEDN when using SEQ ID NO: 1 for numbering. 29. The variant of claim 9 , comprising alterations in positions corresponding to the following positions in SEQ ID NO: 1: W140Y+Y160S W140Y+E194D W140Y+N260P W140Y+G304K W140Y+G304R W140Y+R439T W140Y+W469T W140Y+D476K W140Y+G477R Y160S+E194D Y160S+E212D Y160S+N260P Y160S+G304K Y160S+G304R Y160S+R439T Y160S+W469T Y160S+D476K Y160S+G477R N260P+G304K N260P+G305R N260P+R439K N260P+W469R N260P+D476K N260P+G477R W284G+G304K W284G+G305R W284G+R439N W284G+W469T W284G+D476K W284G+G477R L279T+W284G+H324N+G304K L279T+W284G+H324N+G305R L279T+W284G+H324N+R439N L279T+W284G+H324N+W469T L279T+W284G+H324N+D476K L279T+W284G+H324N+G477R G304K+R439K G304K+W469R G304K+D476K G304K+G477R G305K+R439K G305K+W469R G305K+D476K G305K+G477R R439N+W469R R439N+D476K R439N+G477R W469T+D476K W469T+G477R W140Y+G304R+G475K W140Y+N260P+G304R+G475K W140Y+E212D+G304R+G475K W140Y+G304R+W438R+G475K W140Y+G304R+W468T+G475K W140Y+G305K+G475K W140Y+N260P+G305K+D476K W140Y+E212D+G305K+D476K W140Y+G305K+R439N+D476K W140Y+G305K+W469T+D476K W140Y+G304R+D476K W140Y+N260P+G304R+G477K W140Y+E212D+G304R+G477K W140Y+G304R+R439N+G477K W140Y+G304R+W469T+G477K W140Y+G305K+G477K W140Y+W284K+G305K+G477K W140Y+N260P+G305K+G477K W140Y+E212D+G305K+G477K W140Y+G305K+R439N+G477K and W140Y+G305K+W469T+G477. 30. The variant of claim 9 , wherein the alterations consist of the alterations in positions corresponding to the following positions in SEQ ID NO: 1: W140Y+Y160S W140Y+E194D W140Y+N260P W140Y+G304K W140Y+G304R W140Y+R439T W140Y+W469T W140Y+D476K W140Y+G477R Y160S+E194D Y160S+E212D Y160S+N260P Y160S+G304K Y160S+G304R Y160S+R439T Y160S+W469T Y160S+D476K Y160S+G477R N260P+G304K N260P+G305R N260P+R439K N260P+W469R N260P+D476K N260P+G477R W284G+G304K W284G+G305R W284G+R439N W284G+W469T W284G+D476K W284G+G477R L279T+W284G+H324N +G304K L279T+W284G+H324N +G305R L279T+W284G+H324N +R439N L279T+W284G+H324N +W469T L279T+W284G+H324N +D476K L279T+W284G+H324N +G477R G304K+R439K G304K+W469R G304K+D476K G304K+G477R G305K+R439K G305K+W469R G305K+D476K G305K+G477R R439N+W469R R439N+D476K R439N+G477R W469T+D476K W469T+G477R W140Y+G304R+G475K W140Y+N260P+G304R+G475K W140Y+E212D+G304R+G475K W140Y+G304R+W438R+G475K W140Y+G304R+W468T+G475K W140Y+G305K+G475K W140Y+N260P+G305K+D476K W140Y+E212D+G305K+D476K W140Y+G305K+R439N+D476K W140Y+G305K+W469T+D476K W140Y+G304R+D476K W140Y+N260P+G304R+G477K W140Y+E212D+G304R+G477K W140Y+G304R+R439N+G477K W140Y+G304R+W469T+G477K W140Y+G305K+G477K W140Y+W284K+G305K+G477K W140Y+N260P+G305K+G477K W140Y+E212D+G305K+G477K W140Y+G305K+R439N+G477K and W140Y+G305K+W469T+G477K. 31. The variant of claim 9 , which has at least 95%, but less than 100%, sequence identity to the amino acid sequence of SEQ ID NO: 1. 32. The variant of claim 9 , which has at least 97%, but less than 100%, sequence identity to the amino acid sequence of SEQ ID NO: 1. 33. The variant of claim 9 , which has at least 99%, but less than 100%, sequence identity, to the amino acid sequence of SEQ ID NO: 1. 34. The variant of claim 9 , wherein the parent alpha-amylase comprises or consists of the mature polypeptide of SEQ ID NO: 1. 35. A detergent composition comprising a variant alpha-amylase of claim 1 . 36. The variant of claim 10 , wherein th

Assignees

Novozymes As

Inventors

Andersen Carsten

Classifications

C12Y302/01001
Alpha-amylase (3.2.1.1) · CPC title
C12N9/2414Primary
Alpha-amylase (3.2.1.1.) · CPC title
C11D3/386
Preparations containing enzymes {, e.g. protease or amylase} · CPC title
C12N9/2417
from microbiological source · CPC title

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What does patent US9902946B2 cover?: The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Who is the assignee on this patent?: Novozymes As
What technology area does this patent fall under?: Primary CPC classification C12N9/2414. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?: Publication date Tue Feb 27 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?: We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).