Device for renal cell expansion

Patent metadata

Field	Value
Publication number	US-9902939-B2
Application number	US-200913120323-A
Country	US
Kind code	B2
Filing date	Sep 23, 2009
Priority date	Sep 25, 2008
Publication date	Feb 27, 2018
Grant date	Feb 27, 2018

Abstract

A device for culturing, storing and expanding adherent renal cells. The device permits the adhesion and proliferation of the cells and the surface characteristics of the membrane material used in said device further permit the cellular matrix components (EMC). A method for expanding adherent renal cells and methods of using particular membranes for culturing, storing and expanding adherent renal cells. The membranes comprise a copolymer of acrylonitrile and sodium methallyl sulfonate as well as polyethylenimine.

First claim

The invention claimed is:

1. A method of culturing adherent renal cells comprising providing a membrane comprising a copolymer of acrylonitrile and sodium methallylsulfonate, and further comprising polyethyleneimine, and heparin, and inoculating the membrane with adherent renal cells.

2. A method of culturing primary human renal tubule cells comprising providing a membrane comprising a copolymer of acrylonitrile and sodium methallylsulfonate, and polyethyleneimine, and heparin, and inoculating the membrane with primary human renal tubule cells.

3. A device for culturing, storing and expanding adherent renal cells, comprising a membrane comprising a copolymer of acrylonitrile and sodium methallylsulfonate and further comprising polyethyleneimine and heparin.

4. The device of claim 3 , wherein the membrane is in the form of at least one sheet.

5. The device of claim 3 , wherein the membrane is in the form of at least one hollow fiber.

6. The device of claim 3 further comprising two fluid compartments, wherein the membrane forms an interface between the two fluid compartments.

7. A process for expanding adherent renal cells comprising providing a membrane comprising a copolymer of acrylonitrile and sodium methallylsulfonate and further comprising polyethyleneimine, and heparin, and culturing the adherent renal cells on a surface of the membrane.

8. The process of claim 7 , wherein providing a membrane comprising a copolymer of acrylonitrile and sodium methallylsulfonate and further comprising polyethyleneimine and heparin comprises providing a hollow fiber membrane comprising a copolymer of acrylonitrile and sodium methallylsulfonate and further comprising polyethyleneimine and heparin.

9. The process of claim 7 wherein the culturing comprises:
a) seeding the membrane with adherent renal cells;
b) propagating the adherent renal cells;
c) detaching the adherent renal cells from the membrane; and
d) collecting the detached adherent cells.

10. The process of claim 9 , wherein b) propagating the adherent renal cells is terminated after a confluent layer of adherent renal cells has been formed on the membrane.

11. The process of claim 9 wherein a) seeding the membrane with adherent renal cells b) propagating the adherent renal cells, c) detaching the adherent renal cells from the membrane; and d) collecting the detached adherent cells are repeated using the same membrane.

12. An artificial kidney comprising a membrane comprising a copolymer of acrylonitrile and sodium methallylsulfonate, and further comprising polyethyleneimine, and heparin, and renal cells adhered to the surface of the membrane.

13. The device of claim 4 further comprising two fluid compartments, wherein the membrane forms an interface between the two fluid compartments.

14. The device of claim 5 further comprising two fluid compartments, wherein the membrane forms an interface between the two fluid compartments.

15. The process of claim 8 wherein the culturing comprises:
a) seeding the membrane with adherent renal cells;
b) propagating the adherent renal cells;
c) detaching the adherent renal cells from the membrane ; and
d) collecting the detached adherent cells.

16. The process of claim 15 , wherein b) propagating the adherent renal cells is terminated after a confluent layer of adherent renal cells has been formed on the membrane.

17. The process of claim 10 wherein a) seeding the membrane with adherent renal cells b) propagating the adherent renal cells, c) detaching the adherent renal cells from the membrane; and d) collecting the detached adherent cells are repeated using the same membrane.

Assignees

• Luttropp David
• Neubauer Markus
• Geiger Helmut
• Bereiter Hahn Juergen
• Baer Patrick
• Deppisch Reinhold
• Deppisch Doris
• Gambro Lundia Ab

Inventors

• Luttropp David
• Neubauer Markus
• Geiger Helmut
• Bereiter Hahn Juergen
• Baer Patrick
• Deppisch Reinhold
• Deppisch Doris

Classifications

• C12M21/08

  for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00) · CPC title

• B01D71/68

  Polysulfones; Polyethersulfones · CPC title

• C12N2533/30

  Synthetic polymers · CPC title

• C12M25/10

  Hollow fibers or tubes (hollow fiber modules in general B01D63/02) · CPC title

• C12M29/16

  Hollow fibers (hollow fiber modules in general B01D63/02) · CPC title