Methods, apparatus, and systems for fabrication of polymeric nano- and micro-fibers in aligned configurations

Patent metadata

Field	Value
Publication number	US-9902932-B2
Application number	US-201514683822-A
Country	US
Kind code	B2
Filing date	Apr 10, 2015
Priority date	Jul 31, 2008
Publication date	Feb 27, 2018
Grant date	Feb 27, 2018

Abstract

Provided herein are apparatus and systems for fabricating highly aligned arrays of polymeric fibers having isodiameters ranging from sub 50 nm to microns with lengths of several millimeters. The approach disclosed herein uses (e.g.) a micropipette to deliver polymeric solution which is collected in the form of aligned fibers on a rotating and linearly translating substrate. The methods deposit polymeric fibers on spherical surfaces and gapped surfaces with precise control, thus heralding new opportunities for a variety of applications employing polymeric fibers. The design workspace for depositing fibers disclosed herein is dependent upon processing parameters of rotational/linear translational speeds and material properties of solution rheologies. Techniques for fabrication of multilayer fiber arrays, for fabrication of cell growth scaffolds and for attachment of particles to the fiber arrays are also disclosed.

First claim

I claim:

1. A scaffold comprising one or more layers of one or more high aspect ratio, bead-free polymer fibers having a diameter of between about 10 nm and less than 1000 nm,
wherein the fibers in each of the one or more layers of the scaffold are in a geometrically-spaced configuration,
wherein the polymer is one or more of polystyrene, polyester, polyurethane, polyacrylamide, poly (methyl methacrylate), polylactic acid, poly(lactic-co-glycolic acid), poly(caprolactone), fibrinogen, collagen, and mixtures and copolymers thereof and wherein the fibers are prepared by a method comprising:
determining an entanglement concentration (C e ) for a first polymer solution comprising a first polymer and a first good solvent for the first polymer;
feeding the first polymer solution comprising the first polymer having a concentration of at least C e in the first good solvent for the first polymer through a spinneret to produce an extruded droplet of polymer solution at a tip of the spinneret;
contacting the extruded droplet of polymer solution with a target at a contact point; moving the contact point away from the spinneret, thereby pulling a high aspect ratio polymeric fiber from the extruded droplet of polymer solution at the tip of the spinneret; and further pulling the fiber from the extruded droplet of polymer solution at the tip of the spinneret and feeding the first polymer solution through the spinneret into the extruded droplet of polymer solution at the tip of the spinneret at a rate sufficient to compensate for an amount of the first polymer solution used to produce the fiber, thereby producing a bead-free, high aspect ratio polymeric fiber.

2. The scaffold of claim 1 , wherein the scaffold is further coated with one or more of: collagen, vitronectin, laminin, fibronectin, fibrinogen, poly(ornithine), and poly(lysine).

3. The scaffold of claim 1 , wherein the fibers are coated with collagen.

4. The scaffold of claim 1 , wherein the fibers are coated with poly-L-ornithine.

5. The scaffold of claim 1 , wherein at least one of the one or more layers comprises at least two different fibers.

6. The scaffold of claim 1 , further comprising one or more beaded fibers.

7. The scaffold of claim 1 , wherein the polymer fibers have a diameter between 40 nm and 500 nm.

8. The scaffold of claim 1 , wherein the scaffold comprises a plurality of layers of polymer fibers, and wherein the diameter of and/or spacing between individual fibers is different in at least two of the plurality of layers.

9. The scaffold of claim 1 , wherein the polymer fibers comprise a mixture of fibrinogen and poly(lactic-co-glycolic acid).

10. The scaffold of claim 1 , wherein the polymer fibers comprise polystyrene.

11. The scaffold of claim 1 , wherein the scaffold is three-dimensional.

12. The scaffold of claim 1 , wherein the scaffold is immobilized in a cell culture vessel.

13. The scaffold of claim 12 , wherein the scaffold is immobilized in a tissue culture well.

14. The scaffold of claim 1 , wherein the scaffold comprises two or more layers of fibers,
a first layer of evenly-spaced, parallel fibers, and
a second layer of evenly-spaced, parallel fibers adjacent to the first layer of evenly-spaced, parallel fibers, wherein fibers of the second layer are in a different orientation than fibers of the first layer.

15. A scaffold comprising two or more layers of one or more high aspect ratio, bead-free polymer fibers,
wherein the polymer is one or more of a polystyrene, polyester, polyurethane, polyacrylamide, poly (methyl methacrylate), polylactic acid, poly(lactic-co-glycolic acid), poly(caprolactone), fibrinogen, collagen, and mixtures and copolymers thereof, and wherein the polymer has a molecular weight ranging from 100,000 g/mol to 1,800,000 g/mol, and the fibers are between 40 nm and 500 nm in diameter and wherein the fibers are prepared by a method comprising:
determining an entanglement concentration (C e ) for a first polymer solution comprising a first polymer and a first good solvent for the first polymer;
feeding the first polymer solution comprising the first polymer having a concentration of at least C e in the first good solvent for the first polymer through a spinneret to produce an extruded droplet of polymer solution at a tip of the spinneret;
contacting the extruded droplet of polymer solution with a target at a contact point; moving the contact point away from the spinneret, thereby pulling a high aspect ratio polymeric fiber from the extruded droplet of polymer solution at the tip of the spinneret; and further pulling the fiber from the extruded droplet of polymer solution at the tip of the spinneret and feeding the first polymer solution through the spinneret into the extruded droplet of polymer solution at the tip of the spinneret at a rate sufficient to compensate for an amount of the first polymer solution used to produce the fiber, thereby producing a bead-free, high aspect ratio polymeric fiber.

16. The scaffold of claim 14 , wherein the fibers are between 10 nm and 100 nm in diameter.

17. The scaffold of claim 14 , wherein the polymer has a molecular weight of from 411,000 g/mol to 1,800,000 g/mol.

18. The scaffold of claim 14 , wherein the fibers are between 40 nm and 100 nm in diameter.

19. The scaffold of claim 15 , wherein the fibers are between 40 nm and 100 nm in diameter.

20. The scaffold of claim 14 , wherein the fibers are between 50 nm and 100 nm in diameter.

21. The scaffold of claim 15 , wherein the fibers are between 50 nm and 100 nm in diameter.

22. The scaffold of claim 7 , wherein the fibers are between 40 nm and 100 nm in diameter.

Assignees

• Univ Carnegie Mellon

Inventors

• Nain Amrinder Singh

Classifications

• D10B2321/121

  polystyrene · CPC title

• C12N2537/00

  Supports and/or coatings for cell culture characterised by physical or chemical treatment · CPC title

• C12N2533/54

  Collagen; Gelatin · CPC title

• C12N2513/00

  3D culture · CPC title

• D01D5/04

  Dry spinning methods {(D01D5/0038 takes precedence)} · CPC title