Microarray substrate, microarray, microfluidic system and methods for preparing the same

Patent metadata

Field	Value
Publication number	US-9897602-B2
Application number	US-201414446017-A
Country	US
Kind code	B2
Filing date	Jul 29, 2014
Priority date	Jul 30, 2013
Publication date	Feb 20, 2018
Grant date	Feb 20, 2018

Abstract

A microarray substrate including a piece of fluoropolymer whose surface is modified with polydopamine, in which the polydopamine forms an array of microspots on the surface of the fluoropolymer piece, and allows immobilization of molecules or cells. A microarray including the substrate, a microfluidic system designed for dispensing reagents onto selected locations on the surface of substrates, and a method for preparing the substrate and the microarray, in which a dopamine solution is dispensed onto the fluoropolymer piece using the microfluidic system, and forms an array of polydopamine microspots serving as the reaction sites for microarray analysis.

First claim

What is claimed is:

1. A microarray substrate, comprising a piece of fluoropolymer whose surface is modified with polydopamine, wherein the polydopamine forms an array of microspots on the surface of the fluoropolymer piece by a microfluidic system, and wherein the microfluidic system comprises a channel layer containing microchannels in which a solution can continuously flow, and a bottom layer which contains an array of micropores and is under the channel layer.

2. The microarray substrate of claim 1 , wherein the fluoropolymer piece is made of a fluoropolymer material selected from the group consisting of FEP (fluorinated ethylene propylene), PTFE (polytetrafluoroethylene) and PFA (perfluoroalkoxy).

3. The microarray substrate of claim 1 , wherein the microspots serve as the reaction sites for microarray analysis.

4. The microarray substrate of claim 1 , wherein the polydopamine is functionalized by a functionalization agent selected from the group consisting of thiol and amino groups.

5. The microarray substrate of claim 1 , wherein the polydopamine allows immobilization of proteins, peptides, nucleic acids, oligonucleotides, cells, polymers, small probe molecules or micro/nanoparticles.

6. The microarray substrate of claim 1 , wherein the microarray substrate further comprises a support material underneath the fluoropolymer piece.

7. A microarray comprising the microarray substrate of claim 1 .

8. The microarray of claim 7 , wherein biomolecules, cells and/or micro/nanoparticles are immobilized on the microarray substrate.

9. The microarray of claim 8 , wherein the biomolecules are selected from the group consisting of proteins, peptides, nucleic acids, oligonucleotides, polymers and small probe molecules.

10. The microarray of claim 8 , wherein the cells are attached to the polydopamine, and the microarray is used for performing tissue engineering, stem cell differentiation or cell targeted drug efficacy testing.

11. The microarray of claim 8 , wherein micro/nanoparticles are modified with a thiol or amino group, and can be conjugated onto polydopamine microspots of the microarray substrate.

12. The microarray of claim 9 , wherein the nucleic acids or the oligonucleotides are modified with a thiol or amino group at their ends.

13. A protein or peptide microarray, comprising the microarray substrate of claim 1 , and an immobilized protein or peptide onto the polydopamine, wherein the protein or peptide forms covalent bonding with the polydopamine.

14. A method for producing the microarray substrate of claim 1 , further comprising dispensing a dopamine solution onto a piece of fluoropolymer.

15. The method of claim 14 , wherein the dispensing comprises binding a microfluidic system with the fluoropolymer piece, and keeping the dopamine solution flowing over the surface of fluoropolymer piece which is exposed to the dopamine solution through the micropores of the microfluidic system.

16. A method for preparing the microarray of claim 7 , comprising dispensing a reagent of interest onto the microarray substrate of claim 1 .

17. The method of claim 16 , wherein the dispensing comprises rolling a droplet of the reagent on the surface of the substrate, wherein the reagent can be captured by polydopamine microspots, and thereby forms an array on the substrate surface.

18. The method of claim 16 , wherein the dispensing comprises placing a microfluidic system above the substrate surface, and wherein the reagent is introduced into the channels of the microfluidic system and flows over the microspots of the substrate.

19. The method of claim 16 , wherein the reagent is selected from the group consisting of proteins, peptides, nucleic acids, oligonucleotides, cells, polymers, small probe molecules and micro/nanoparticles.

20. A method for preparing a protein or peptide microarray of claim 13 , comprising dispensing a protein or peptide solution onto the microarray substrate of any claim 1 , and incubating the protein or peptide solution, wherein the protein or peptide forms covalent bonding with the polydopamine and is preferably conjugated onto the polydopamine microspots.

21. A method for detecting a substance of interest in a sample using the microarray claim 7 , comprising dispensing the sample on the microarray and detecting the binding of the substance of interest and the microarray.

22. The method of claim 21 , wherein the substance of interest is selected from the group consisting of proteins, peptides, nucleic acids, oligonucleotides, cells, drug compounds and micro/nanoparticles.

23. The method of claim 21 , wherein the binding of the substance and the microarray is detected via colorimetry, fluorescence, luminescence, electrochemical signals, mass spectrometry, or radioactivity signals.

Assignees

• Univ Hong Kong Chinese

Inventors

• Zheng Bo
• Feng Hui

Classifications

• C40B40/02

  Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors · CPC title

• G01N33/54353

  with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393) · CPC title

• C40B40/10

  Libraries containing peptides or polypeptides, or derivatives thereof · CPC title

• C40B40/04

  Libraries containing only organic compounds · CPC title

• G01N33/54393 Primary

  Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding · CPC title