Systems and trays for processing samples by a robotic platform

Patent metadata

Field	Value
Publication number	US-9896653-B2
Application number	US-201715460678-A
Country	US
Kind code	B2
Filing date	Mar 16, 2017
Priority date	Jan 11, 2013
Publication date	Feb 20, 2018
Grant date	Feb 20, 2018

Abstract

A system for processing samples by a robotic platform has a tray that defines a well defining a drain. The well has a bottom surface, an access port in fluid communication with the drain, and an insert adapted to be received in the well. The insert has a bottom wall and a side wall. At least one of the bottom wall and the side wall define a plurality of openings, such that an interior of the insert is in fluid communication with the well when the insert is inserted into the well.

First claim

What is claimed is:

1. A system for processing samples, the system comprising:
a tray defining:
a well defining a drain, wherein the well comprises a bottom surface; and
an access port in fluid communication with the drain; and

an insert adapted to be received in the well,
wherein the insert comprises a bottom wall and a side wall, wherein at least one of the bottom wall and the side wall define a plurality of openings, such that an interior of the insert is in fluid communication with the well when the insert is inserted into the well, and wherein at least some of the openings define an angle relative to the side wall or the bottom wall so as to induce a current or swirl in the fluid.

2. The system of claim 1 , wherein the well defines a channel connecting the drain and the access port, the channel including an approximate 90 degree turn between the drain and access port.

3. The system of claim 1 , wherein the drain defines a drain diameter (D D ) and the access port defines an access port diameter (D A ), wherein the drain diameter (D D ) is larger than the access port diameter (D A ), wherein the insert comprises a bottom wall and a side wall.

4. The system of claim 1 , wherein the access port is discrete from the well.

5. The system of claim 1 , wherein both the bottom wall and the side wall of the insert define a plurality of openings, and wherein the openings in the bottom wall and the openings in the side wall define a curved shape.

6. The system of claim 5 , wherein the openings in the bottom wall and the openings in the side wall define a circular shape.

7. The system of claim 1 , wherein both the bottom wall and the side wall of the insert define a plurality of openings, and wherein the openings in the bottom wall have a different shape than the openings in the side wall.

8. The system of claim 7 , wherein the openings in the side wall define a rectangular shape and the openings in the bottom wall define a curved shape.

9. The system of claim 1 , wherein the insert is treated with a chemical treatment that renders the outer surfaces of the insert non-porous.

10. The system of claim 1 , wherein the insert is treated so as to render the insert capable of releasing growth factors or cytokines.

11. A basket for use with a system for processing samples, the system including a tray defining a well, the basket comprising:
a bottom wall and a side wall, wherein at least one of the bottom wall and the side wall define a plurality of openings, such that an interior of the basket is configured to be in fluid communication with the well of the tray, and wherein at least some of the openings define an angle relative to the side wall or the bottom wall so as to induce a current or swirl in a fluid introduced into the basket.

12. The basket of claim 11 , wherein both the bottom wall and the side wall define a plurality of openings.

13. The basket of claim 12 , wherein the openings in the bottom wall and the openings in the side wall define a curved shape.

14. The basket of claim 13 , wherein the openings in the bottom wall and the openings in the side wall define a circular shape.

15. The basket of claim 12 , wherein the openings in the bottom wall have a different shape than the openings in the side wall.

16. The basket of claim 15 , wherein the openings in the side wall define a rectangular shape and the openings in the bottom wall define a curved shape.

17. The basket of claim 11 , wherein the basket defines an overall shape that is complementary to an outer wall shape of the well.

18. The basket of claim 17 , wherein the overall shape of the basket is at least one of a circular shape, a quadrilateral shape, an oval shape, and a triangular shape.

19. The basket of claim 11 , wherein the basket is treated with a chemical treatment that renders the outer surface of the basket non-porous.

20. The basket of claim 11 , wherein the basket is treated so as to render the basket capable of releasing growth factors or cytokines.

21. A method of utilizing a tray and basket system for processing samples, the method comprising:
inserting a basket into a tray, the tray having a well defining a drain that includes a bottom surface, and an access port in fluid communication with the drain;
inserting an embryo into the basket;
inserting the loaded tray into robotic processing equipment;
inserting a pipette into the access port;
introducing and/or withdrawing fluid into the basket via the pipette;
removing the tray from the robotic processing equipment; and
removing the embryo from the basket,
wherein the step of introducing and/or withdrawing fluid into the basket comprises inducing a current or swirl in the fluid due to a configuration of the basket.

22. The method of claim 21 , wherein at least one of the steps, including the step of inserting the basket into the tray, is performed manually.

23. The method of claim 21 , wherein at least one of the steps, including the step of inserting the basket into the tray, is performed by robotic equipment.

24. The method of claim 21 , wherein at least one of the steps, including the step of removing the embryo from the basket, is performed manually.

25. The method of claim 21 , wherein at least one of the steps, including the step of removing the embryo from the basket, is performed by robotic equipment.

26. The method of claim 21 , further comprising treating the basket with a chemical solution configured to render the outer surface of the basket non-porous, prior to the step of inserting the basket into the tray.

27. The method of claim 21 , further comprising treating the basket so as to render the basket capable of releasing growth factors or cytokines, prior to the step of inserting the basket into the tray.

28. The method of claim 21 , wherein the basket comprises a bottom wall and a side wall, and wherein both the bottom wall and the side wall define a plurality of openings so as to induce the current or swirl in the fluid.

29. The method of claim 28 , wherein the openings in the bottom wall and the openings in the side wall define a curved shape.

30. The method of claim 29 , wherein the openings in the bottom wall and the openings in the side wall define a circular shape.

31. The method of claim 28 , wherein the openings in the bottom wall have a different shape than the openings in the side wall.

32. The method of claim 31 , wherein the openings in the side wall define a rectangular shape and the openings in the bottom wall define a curved shape.

33. The method of claim 21 , further comprising sealing the basket and tray assembly, after the step of inserting the basket into the tray.

34. The method of claim 33 , further comprising removing the seal from the basket and tray assembly, prior to the step of inserting an embryo into the basket.

Assignees

• Regeneron Pharma

Inventors

• Chernomorsky Rostislav
• Gale Nicholas
• Cichon Sam

Classifications

• B01L3/5085 Primary

  for multiple samples, e.g. microtitration plates · CPC title

• B01L2300/0609

  Holders integrated in container to position an object · CPC title

• B01L2200/026

  Fluid interfacing between devices or objects, e.g. connectors, inlet details · CPC title

• G01N1/31

  Apparatus therefor · CPC title

• B01L2300/0681

  Filter · CPC title