Circuit-based optoelectronic tweezers

Patent metadata

Field	Value
Publication number	US-9895699-B2
Application number	US-201615207210-A
Country	US
Kind code	B2
Filing date	Jul 11, 2016
Priority date	Nov 8, 2012
Publication date	Feb 20, 2018
Grant date	Feb 20, 2018

Abstract

A microfluidic optoelectronic tweezers (OET) device can comprise dielectrophoresis (DEP) electrodes that can be activated and deactivated by controlling a beam of light directed onto photosensitive elements that are disposed in locations that are spaced apart from the DEP electrodes. The photosensitive elements can be photodiodes, which can switch the switch mechanisms that connect the DEP electrodes to a power electrode between an off state and an on state.

First claim

We claim:

1. A microfluidic apparatus, comprising:
a circuit substrate comprising an inner surface;
an electrically conductive terminal on the inner surface;
a switch mechanism that connects the electrically conductive terminal to a first power electrode in a first on state and disconnects the electrically conductive terminal from the first power electrode in an off state; and
a photosensitive element that connects to the switch mechanism, wherein an output of the photosensitive element controls whether the switch mechanism is in the first on state and the off state.

2. The apparatus of claim 1 , wherein the photosensitive element is on the inner surface and the electrically conductive terminal is spaced apart from the photosensitive element on the inner surface.

3. The apparatus of claim 1 , wherein the electrically conductive terminal is disposed, at least partially, around the photosensitive element.

4. The apparatus of claim 1 , wherein the electrically conductive terminal is transparent to light and the electrically conductive terminal covers the photosensitive element.

5. The apparatus of claim 1 , wherein the inner surface defines part of a chamber and the chamber comprises a liquid medium.

6. The apparatus of claim 1 , wherein the output of the photosensitive element is received by a control circuitry that toggles the switch mechanism between the first on state and the off state responsive to the output of the photosensitive element.

7. The apparatus of claim 1 , wherein the photosensitive element comprises a photodiode.

8. The apparatus of claim 7 , wherein the photodiode is configured to provide the output in response to a color of light.

9. The apparatus of claim 1 , wherein the photosensitive element is configured to provide the output in response to one or more pulses of light.

10. The apparatus of claim 1 , further comprising a color filter configured to pass a specific color of light to the photosensitive element.

11. The apparatus of claim 1 , wherein the switch mechanism comprises a transistor.

12. The apparatus of claim 11 , wherein the transistor is selected from the group of: a field effect transistor, a bipolar transistor and a bi-MOS transistor.

13. The apparatus of claim 1 , wherein the photosensitive element comprises a photodiode and the switch mechanism comprises an amplifier.

14. The apparatus of claim 13 , wherein the switch mechanism further comprises a switch in series with the amplifier.

15. The apparatus of claim 1 , further comprising a second power electrode, wherein the switch mechanism connects the electrically conductive terminal to the second power electrode in a second on state and disconnects the electrically conductive terminal from the second power electrode in the off state.

16. The apparatus of claim 15 , further comprising a third power electrode, wherein the switch mechanism connects the electrically conductive terminals to the third power electrode in a third on state and disconnects the electrically conductive terminal from the third power electrode in the off state.

17. The apparatus of claim 1 , further comprising a second power electrode and wherein the switch mechanism connects the electrically conductive terminal to the first power electrode in the first on state and connects the electrically conductive terminal to the second power electrode in the off state.

18. A microfluidic apparatus, comprising:
a circuit substrate comprising an inner surface;
a chamber configured to contain a liquid medium disposed on the inner surface;
a switch mechanism located in a region of the inner surface that is in electrical contact with the liquid medium and connected to a power electrode in an on state and disconnected from the power electrode in an off state; and
a photosensitive element on the inner surface that connects to the switch mechanism and controls whether the switch mechanism is in the on state and the off state.

19. A method of controlling a microfluidic device comprising a circuit substrate, a photosensitive element disposed on an inner surface of the circuit substrate and an electrically conductive terminal disposed on the inner surface of the circuit substrate, the method comprising:
selectively directing light onto the photosensitive element, wherein the photosensitive element generates an output responsive to the light directed onto the photosensitive element;
switching a switch mechanism between an on state and an off state responsive to the output generated by the photosensitive element, wherein the switch mechanism connects the electrically conductive terminals to a first power electrode in the on state and disconnects the electrically conductive terminal from the first power electrode in the off state.

20. The method of claim 19 , wherein the microfluidic device further comprises control circuitry that connects the photosensitive element to the switch mechanism, and wherein switching the switch mechanism between the on state and the off state comprises the control circuitry:
receiving the output generated by the photosensitive element; and
providing an input to the switch mechanism responsive to the output received from the photosensitive element.

21. The method of claim 20 , wherein:
selectively directing light onto the photosensitive element comprises directing one or more pulses of light onto the photosensitive element, wherein the photosensitive element generates a pulse of positive signal output responsive to the one or more pulses of light; and
switching the switch mechanism between the on state and the off state is responsive to the pulse of positive signal output.

22. The method of claim 21 , wherein:
selectively directing light onto the photosensitive element comprises directing a pattern of pulses of light onto the photosensitive element, wherein the photosensitive element generates a pulse of positive signal output responsive to the pattern of pulses of light; and
switching the switch mechanism between the on state and the off state is responsive to the pulse of positive signal output.

23. The method of claim 22 , wherein:
selectively directing light onto the photosensitive element comprises directing a color of light onto the photosensitive element, wherein the photosensitive element generates an output responsive to the color of light directed onto the photosensitive element;
switching the switch mechanism between the on state and the off state is responsive to the output generated by the photosensitive element.

Assignees

• Berkeley Lights Inc

Inventors

• Short Steven W
• Wu Ming C

Classifications

• B01L3/502761

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• B03C5/005 Primary

  Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength · CPC title

• B03C5/026

  using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC] · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title

• B01L2400/0424

  Dielectrophoretic forces · CPC title