Labeled circular DNA molecules for analysis of DNA topology, and topoisomerases and for drug screening

Patent metadata

Field	Value
Publication number	US-9890416-B2
Application number	US-201615286046-A
Country	US
Kind code	B2
Filing date	Oct 5, 2016
Priority date	Oct 5, 2015
Publication date	Feb 13, 2018
Grant date	Feb 13, 2018

Abstract

The present invention provides labeled circular plasmid DNA molecules for studying DNA topology and topoisomerases. The molecules of the present invention also provide tools for high throughput drug screening for inhibitors of DNA gyrases and DNA topoisomerases for anticancer drug discovery and antibiotics discovery.

First claim

The invention claimed is:

1. A nucleic acid sequence comprising:
an adenosine-thymidine dinucleotide repeat (AT) n sequence, characterized in that n is at least 12, the (AT) n sequence comprises at least one fluorophore and at least one quencher, each conjugated to a deoxythymidine (dT), and the fluorophore-conjugated dT and the quencher-conjugated dT are separated by at least 14 nucleotides.

2. The nucleic acid sequence according to claim 1 , characterized in that the fluorophore and the quencher are on the same DNA strand.

3. The nucleic acid sequence according to claim 1 , further comprising at least one nicking endonuclease recognition site.

4. The nucleic acid sequence according to claim 1 , further comprising at least one DNA endonuclease recognition site.

5. The nucleic acid sequence according to claim 1 , further comprising at least one RNA oligomer.

6. The nucleic acid sequence according to claim 1 , characterized in that n is from about 12 to about 17; from about 18 to about 25; from about 26 to about 33; from about 34 to about 41; or from about 42 to about 50.

7. The nucleic acid sequence according to claim 2 , characterized in that the fluorophore is conjugated to a dT located at the fourth; the fifth; the sixth; the seventh; the eighth; the ninth; the tenth; the eleventh; the twelfth; the thirteenth; or the fourteenth position from the 5′ start of the (AT) n sequence.

8. The nucleic acid sequence according to claim 2 , characterized in that the fluorophore is conjugated to a dT located at the 28th; 29th; 30th; the 31st; the 32nd; the 33rd; the 34th; the 35h; the 36th; the 37th; the 38th; the 39th; or the 40th position from the 5′ start of the (AT) n sequence.

9. The nucleic acid sequence according to claim 7 , characterized in that the quencher is conjugated to a dT located at the 28th; 29th; 30th; the 31st; the 32nd; the 33rd; the 34th; the 35h; the 36th; the 37th; the 38th; the 39th; or the 40th position from the 5′ start of the (AT) n sequence.

10. The nucleic acid sequence according to claim 8 , characterized in that the quencher is conjugated to a dT located at the fourth; the fifth; the sixth; the seventh; the eighth; the ninth; the tenth; the eleventh; the twelfth; the thirteenth; or the fourteenth position from the 5′ start of the (AT) n sequence.

11. A nucleic acid sequence comprising:
an adenosine-thymidine dinucleotide repeat (AT)n sequence, characterized in that n is 21, the (AT) n sequence comprises one fluorophore and one quencher, each conjugated to a deoxythymidine (dT), and the fluorophore-conjugated dT and the quencher-conjugated dT are on the same strand and are separated by 25 nucleotides.

12. The nucleic acid sequence according to claim 1 , characterized in that the distance between the fluorophore and the quencher is from about 14 to about 16 nucleotides; from about 17 to about 20 nucleotides; from about 21 to about 23 nucleotides; from 24 to about 26 nucleotides; and from about 27 to about 32 nucleotides.

13. The nucleic acid sequence according to claim 1 , characterized in that the fluorophore is 6-FAM (fluoroscein), cyanine 3, tetramethylrhodamine, carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein (JOE), cyanine 5, cyanine 5.5, tetrachloro-fluorescein phosphoramidite (TET), carboxy-X-Rhodamine, Hexachlorofluorescein, 5-tetramethylrhodamine, WEllRED D2, WellRED D3, WellRED D4, 5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid (EDANS), or 5-[2-[(2-Iodo-1-oxoethyl)amino]ethylamino]-1-naphthalenesulfonic acid (IAEDANS).

14. The nucleic acid sequence according to claim 1 , characterized in that the quencher is 4-((4-(dimethylamino)phenyl)azo)benzoic Acid (Dabcyl), 2,3-Dichloro-5,6-dicyano-1,4-benzoquinone (DDQ)-I, Black Hole Quencher (BHQ)-1, BHQ-2, DDQ-II, BHQ-3, or 2-[6-(1,3-dihydro-2H-isoindol-2-yl)-9-{2-[(4-[(2,5-dioxopyrrolidin-1-yl)oxy] carbonyl piperidin-1-yl)sulfonyl]phenyl}-3H-xanthen-3-ylidene]-2,3-dihydro-1H-isoindolium chloride (QSY 21).

Assignees

• Leng Fenfei
• The Florida International Univ Board Of Trustees

Inventors

• Leng Fenfei

Classifications

• C07H21/00

  Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids · CPC title

• C12Q1/6818 Primary

  involving interaction of two or more labels, e.g. resonant energy transfer · CPC title