Compositions and methods for producing isoprene
US-9260727-B2 · Feb 16, 2016 · US
Modified isoprene synthase
US9890373B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9890373-B2 |
| Application number | US-201615159140-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 19, 2016 |
| Priority date | Nov 22, 2013 |
| Publication date | Feb 13, 2018 |
| Grant date | Feb 13, 2018 |
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Abstract
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The present application relates to a modified isoprene synthase that has an isoprene synthetic activity and has at least one mutation of an amino acid residue in the amino acid sequence of SEQ ID NO: 4, an amino acid sequence having one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NO: 4, or an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO: 4. The modified isoprene synthase is useful for preparing isoprene monomers in improved yields.
First claim
Opening claim text (preview).
The invention claimed is: 1. A modified isoprene synthase, having an amino acid sequence at least 90 identical to the amino acid sequence of SEQ ID NO: 4, wherein the modified isoprene synthase has at least one mutation of an amino acid residue corresponding to at least one amino acid residue in SEQ ID NO: 4 selected from the group consisting of: C286, E321, C370, A390, E471, C480, I518, and C521, and the modified isoprene synthase has an isoprene synthetic activity. 2. The modified isoprene synthase of claim 1 , wherein the at least one mutation in the modified isoprene synthase corresponds to at least one mutation in SEQ ID NO: 4 selected from the group consisting of: C286V, E321D, C370E, A390C, E471C, C480N, I518C, C521V, and C521I. 3. The modified isoprene synthase of claim 1 , wherein the modified isoprene synthase further comprises a signal sequence at the N-terminus. 4. A modified isoprene synthase, having an amino acid sequence at least 90% identical to the amino acid sequence of SEQ ID NO: 2, wherein the modified isoprene synthase has at least one mutation of an amino acid residue corresponding to at least one amino acid residue in SEQ ID NO: 2 selected from the group consisting of: C329, E364, C413, A433, E514, C523, I561, and C564, and the modified isoprene synthase has an isoprene synthetic activity. 5. The modified isoprene synthase of claim 4 , wherein the at least one mutation in the modified isoprene synthase corresponds to at least one mutation in SEQ ID NO: 2 selected from the group consisting of: C329V, E364D, A433C, E514C, C523N, I561C, C564V, and C564I. 6. The modified isoprene synthase of claim 1 , wherein the amino acid sequence of the modified isoprene synthase has 95% or more identity to the amino acid sequence of SEQ ID NO: 4. 7. The modified isoprene synthase of claim 1 , wherein the at least one mutation in the modified isoprene synthase corresponds to at least one mutation in SEQ ID NO: 4 selected from the group consisting of: C286I, C286V, E321D, C370K, A390C, E471C, C480N, I518C, C521V, and C521I. 8. The modified isoprene synthase of claim 1 , wherein the at least one mutation in the modified isoprene synthase corresponds to at least two mutations in SEQ ID NO: 4 selected from the group consisting of: C286I, C286V, E321D, C370K, A390C, E471C, C480N, I518C, C521V, and C521I. 9. The modified isoprene synthase of claim 2 , wherein the modified isoprene synthase further comprises a signal sequence at the N-terminus.
Assignees
Inventors
Classifications
Pentalenene synthase (4.2.3.7) · CPC title
Isoprene synthase (4.2.3.27) · CPC title
Carbon · CPC title
- C12N9/88Primary
Lyases (4.) · CPC title
of zinc · CPC title
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Frequently asked questions
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- What does patent US9890373B2 cover?
- The present application relates to a modified isoprene synthase that has an isoprene synthetic activity and has at least one mutation of an amino acid residue in the amino acid sequence of SEQ ID NO: 4, an amino acid sequence having one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NO: 4, or an amino acid sequence having 90% or more…
- Who is the assignee on this patent?
- Ajinomoto Kk
- What technology area does this patent fall under?
- Primary CPC classification C12N9/88. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 13 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).