Soybean markers linked to SCN resistance

Patent metadata

Field	Value
Publication number	US-9888636-B2
Application number	US-201113287848-A
Country	US
Kind code	B2
Filing date	Nov 2, 2011
Priority date	Nov 5, 2010
Publication date	Feb 13, 2018
Grant date	Feb 13, 2018

Abstract

This disclosure concerns compositions and methods for identifying the SCN resistant phenotype in soybean. In some embodiments, the disclosure concerns methods for performing marker-assisted breeding and selection of plants carrying one or more determinants of SCN resistance in soybean.

First claim

What may be claimed is:

1. An oligonucleotide probe selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:7, SEQ ID NO:8, and SEQ ID NO:9, wherein the probe comprises a label that is a fluorophore or 32 P.

2. An oligonucleotide probe that is specifically hybridizable to a marker linked to the SCN resistance phenotype in soybean, wherein the probe is:
an oligonucleotide that consists of SEQ ID NO:11, SEQ ID NO:12, or the complement or reverse complement of either of the foregoing,
wherein the probe comprises a label that is a fluorophore or 32 P.

3. A method for transferring a determinant of SCN resistance in a soybean variety, the method comprising:
(a) sexually crossing a first parental plant having a donor genotype comprising the determinant of SCN resistance with a second parental plant having a recipient genotype to obtain a progeny plant population;
(b) identifying from the progeny plant population a plant with genomic DNA that binds the oligonucleotide probe of claim 2 under very high stringency hybridization conditions, thereby determining the presence of the determinant of SCN resistance in the plant;
(c) backcrossing the identified plant from the progeny population that comprises the determinant of SCN resistance to the recipient genotype to produce a next generation population;
(d) determining if a member of the next generation population comprises genomic DNA that binds the oligonucleotide probe under very high stringency hybridization conditions and a desired trait from the recipient genotype; and
(e) if no member of the next generation population comprises genomic DNA that binds the oligonucleotide probe under very high stringency hybridization conditions and the desired trait from the recipient genotype, repeating steps (c) and (d) until an individual is identified that comprises genomic DNA that binds the oligonucleotide probe under very high stringency hybridization conditions and the desired trait from the recipient genotype.

4. The oligonucleotide probe of claim 2 , wherein the probe is an oligonucleotide that binds under very high stringency conditions to a reference polynucleotide or the perfect complement of the reference polynucleotide, wherein the reference polynucleotide consists of SEQ ID NO:11 comprising a thymine (T) at nucleotide position 98 of the reference polynucleotide, wherein the oligonucleotide probe does not bind under the very high stringency hybridization conditions to the reference polynucleotide comprising a cytosine (C) at nucleotide position 98 of the reference polynucleotide.

5. The oligonucleotide probe of claim 2 , wherein the probe is an oligonucleotide that binds under very high stringency hybridization conditions to a second reference polynucleotide or the perfect complement of the second reference polynucleotide, wherein the second reference polynucleotide consists of SEQ ID NO:12 comprising a cytosine (C) at nucleotide position 214 of the second reference polynucleotide, wherein the oligonucleotide probe does not bind under the very high stringency hybridization conditions to the second reference polynucleotide comprising an adenine (A) at nucleotide position 214 of the second reference polynucleotide.

6. The method according to claim 3 , wherein the probe is an oligonucleotide that binds under very high stringency conditions to a reference polynucleotide or the perfect complement of the reference polynucleotide, wherein the reference polynucleotide consists of SEQ ID NO:11 comprising a thymine (T) at nucleotide position 98 of the reference polynucleotide, wherein the oligonucleotide probe does not bind under the very high stringency hybridization conditions to the reference polynucleotide comprising a cytosine (C) at nucleotide position 98 of the reference polynucleotide.

7. The method according to claim 3 , wherein the probe is an oligonucleotide that binds under very high stringency hybridization conditions to a second reference polynucleotide or the perfect complement of the second reference polynucleotide, wherein the second reference polynucleotide consists of SEQ ID NO:12 comprising a cytosine (C) at nucleotide position 214 of the second reference polynucleotide, wherein the oligonucleotide probe does not bind under the very high stringency hybridization conditions to the second reference polynucleotide comprising an adenine (A) at nucleotide position 214 of the second reference polynucleotide.

8. A method for introducing a determinant of SCN resistance in a soybean variety into a host soybean plant lacking the determinant of SCN resistance by genetic transformation, the method comprising:
analyzing the genomic DNA of a plant with the oligonucleotide probe of claim 2 to identify the determinant of SCN resistance in the plant, wherein binding of the probe to the genomic DNA of the plant confirms the presence of the determinant of SCN resistance in the plant;
isolating a segment of the genomic DNA of the plant that binds to the oligonucleotide probe under very stringent hybridization conditions; and
introducing the isolated segment of genomic DNA into a host soybean plant lacking the determinant of SCN resistance.

9. The method according to claim 8 , wherein the isolated segment of DNA is stably integrated into the genome of the host soybean plant.

10. The method according to claim 8 , wherein the probe is an oligonucleotide that binds under very high stringency conditions to a reference polynucleotide or the perfect complement of the reference polynucleotide, wherein the reference polynucleotide consists of SEQ ID NO:11 comprising a thymine (T) at nucleotide position 98 of the reference polynucleotide, wherein the oligonucleotide probe does not bind under the very high stringency hybridization conditions to the reference polynucleotide comprising a cytosine (C) at nucleotide position 98 of the reference polynucleotide.

11. The method according to claim 8 , wherein the probe is an oligonucleotide that binds under very high stringency hybridization conditions to a second reference polynucleotide or the perfect complement of the second reference polynucleotide, wherein the second reference polynucleotide consists of SEQ ID NO:12 comprising a cytosine (C) at nucleotide position 214 of the second reference polynucleotide, wherein the oligonucleotide probe does not bind under the very high stringency hybridization conditions to the second reference polynucleotide comprising an adenine (A) at nucleotide position 214 of the second reference polynucleotide.

Assignees

• Bai Yonghe
• Lu Fang
• Greene Thomas W
• Moore Robert E
• Hedges Bradley
• Kumpatla Siva P
• Ram Raghav
• Agrigenetics Inc

Inventors

• Bai Yonghe
• Lu Fang
• Greene Thomas W
• Moore Robert E
• Hedges Bradley
• Kumpatla Siva P
• Ram Raghav

Classifications

• C12Q2600/13

  Plant traits · CPC title

• A01H5/10

  Seeds · CPC title

• A01H1/04 Primary

  Processes of selection {involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection} · CPC title

• C12Q1/6895 Primary

  for plants, fungi or algae · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title