Method for staining a histological sample, and automated stainer

Patent metadata

Field	Value
Publication number	US-9885641-B2
Application number	US-201615262323-A
Country	US
Kind code	B2
Filing date	Sep 12, 2016
Priority date	Sep 13, 2012
Publication date	Feb 6, 2018
Grant date	Feb 6, 2018

Abstract

The invention relates inter alia to an automated stainer for staining, in particular for hemalum-eosin (HE) staining, of a histological sample, the automated stainer exposing the sample to the action of at least one stain using at least one staining parameter. The automated stainer is notable for the fact that the automated stainer comprises an input means with which a staining parameter is definable, in particular is inputtable or is selectable from a plurality of possible staining parameters; and that a control apparatus is present which, upon application of the defined staining parameter, ascertains the prospectively expectable outcome of an action and displays it to the user with a display apparatus, before the automated stainer actually stains the sample.

First claim

What is claimed is:

1. A method for staining a histological sample, comprising:
(A) defining a staining parameter to be used in application of a stain to the sample; and
(B) ascertaining and displaying a prospective expected outcome of an action of the stain using the staining parameter before the sample is actually stained, wherein the ascertaining includes at least one of retrieving the prospective expected outcome from a memory or precalculating, and wherein a control apparatus is programmed to perform the ascertaining and the displaying.

2. The method according to claim 1 , further comprising:
(C) adjusting the staining parameter; and
(D) repeating the step of (B) ascertaining and displaying a prospective expected outcome of an action of the stain using the staining parameter before the sample is actually stained;
wherein steps (C) and (D) are performed one or more times until the prospective expected outcome is coincident with a target outcome.

3. The method according to claim 1 , further comprising:
making an assessment whether the prospective expected outcome is coincident with a target outcome; and
initiating an actual staining operation by applying the stain using the staining parameter to the sample when the prospective expected outcome is coincident with the target outcome.

4. The method according to claim 1 , wherein the prospective expected outcome is ascertained in consideration of at least one property of the sample comprising at least one of a tissue type, a tissue size, a tissue density, a tissue thickness, a fat content, and a water content.

5. The method according to claim 1 , wherein the prospective expected outcome is retrieved from the memory and the memory stores at least one of:
(i) a plurality of expected outcomes;
(ii) a plurality of expected outcomes, the staining parameter or a combination of the staining parameters being stored in association with each of the plurality of expected outcomes; and
(iii) a plurality of expected outcomes, at least one sample property being stored in association with each of the plurality of expected outcomes.

6. The method according to claim 1 , wherein the prospective expected outcome is displayed on a screen using a synoptic image of the sample.

7. The method according to claim 6 , wherein the synoptic image of the sample is generated by at least one of optical scanning of the sample, dark-field illumination of the sample, and use of a phase contrast method.

8. The method according to claim 1 , wherein the prospective expected outcome is displayed on a screen in the form of an exemplary view of an exemplary sample different from the sample.

9. The method according to claim 1 , wherein the staining parameter is selected from several displayed staining parameters.

10. The method according to claim 1 , wherein the staining parameter relates to at least one of a stain intensity, an age of the stain, a chemical composition of the stain, and a duration of action of the stain.

Assignees

• Leica Biosystems Nussloch Gmbh

Inventors

• Berberich Markus

Classifications

• G01N35/00663

  of consumables · CPC title

• G01N2035/00673

  of reagents · CPC title

• G01N1/30 Primary

  Staining; Impregnating {; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis} · CPC title

• G01N1/31

  Apparatus therefor · CPC title