Chip for analysis of target substance

Patent metadata

Field	Value
Publication number	US-9885077-B2
Application number	US-201615130601-A
Country	US
Kind code	B2
Filing date	Apr 15, 2016
Priority date	Mar 21, 2012
Publication date	Feb 6, 2018
Grant date	Feb 6, 2018

Abstract

The present invention provides a chip for analysis of a target substance that is compact and allows analysis of a target substance with less time and effort. The chip for analysis of a target substance includes a first flexible substrate 1 , a second flexible substrate 2 , and a third substrate 3 . A flow channel-forming non-bonded area 11 is formed on a bonding surface of the first flexible substrate 1 and the second flexible substrate 2 in a band-like manner and an extraction chamber-forming non-bonded area 5 having a wider band width than the flow channel-forming non-bonded area 11 is formed at a part of the flow channel-forming non-bonded area 11 . The first flexible substrate 1 includes a through-hole 7 that is in contact with the flow channel-forming non-bonded area 11 , a shutter-forming non-bonded area 12 b is formed on a bonding surface of the second flexible substrate 2 and the third substrate 3 at a far side of the through-hole 7 relative to the extraction chamber-forming non-bonded area 5 such that the shutter-forming non-bonded area 12 b and the flow channel-forming non-bonded area 11 intersect above and below, the first flexible substrate 1 and the second flexible substrate 2 include a pressure supply port 18 b that comes through the substrates so as to be in contact with the shutter-forming non-bonded area 12 b , and a magnetic particle 16 that binds to a target substance is placed above the extraction chamber-forming non-bonded area 5.

First claim

The invention claimed is:

1. A chip for analysis of a target substance comprising:
a laminate; and
a magnetic field generating unit configured to generate a magnetic field,
the laminate comprising:
a first flexible substrate;
a second flexible substrate; and
a third substrate, wherein

a flow channel-forming non-bonded area is formed on a bonding surface of the first flexible substrate and the second flexible substrate in a band-like manner and an extraction chamber-forming non-bonded area having a wider band width than the flow channel-forming non-bonded area is formed at a part of the flow channel-forming non-bonded area,
the first flexible substrate comprises a through-hole that is in contact with the flow channel-forming non-bonded area,
a shutter-forming non-bonded area is formed on a bonding surface of the second flexible substrate and the third substrate in a band-like manner at a far side of the through-hole relative to the extraction chamber-forming non-bonded area such that the shutter-forming non-bonded area and the flow channel-forming non-bonded area intersect via the second flexible substrate being interposed therebetween,
at least one of both of the first flexible substrate and the second flexible substrate and the third substrate comprises a pressure supply port that comes through the substrate(s) so as to be in contact with the shutter-forming non-bonded area,
a magnetic particle that binds to a target substance is placed above the extraction chamber-forming non-bonded area,
a flow channel and an extraction chamber are formable by supplying pressure from the through-hole to raise a site above the flow channel-forming non-bonded area and a site above the extraction chamber-forming non-bonded area,
the flow channel is blockable by supplying pressure from the pressure supply port to raise a site above the shutter-forming non-bonded area,
the magnetic field generating unit is disposed at 1) an outer side of at least one of the first flexible substrate or the third flexible substrate, and 2) in a vicinity of an end of the extraction chamber at the opposite side of the through-hole, and
the target substance that is bound to the magnetic particle is capturable by the magnetic field at least one of an undersurface of the third substrate directly below an end of the extraction chamber at the opposite side of the through-hole or a top surface of the first flexible substrate directly above an end of the extraction chamber at the opposite side of the through-hole.

2. The chip according to claim 1 , further comprising:
a PCR amplification portion; and
an electrophoresis analysis portion.

3. A system for analysis of a target substance comprising:
a chip; and
a magnetic field generating unit configured to generate a magnetic field,
the chip comprising a laminate, the laminate comprising:
a first flexible substrate;
a second flexible substrate; and
a third substrate, wherein

a flow channel-forming non-bonded area is formed on a bonding surface of the first flexible substrate and the second flexible substrate in a band-like manner and an extraction chamber-forming non-bonded area having a wider band width than the flow channel-forming non-bonded area is formed at a part of the flow channel-forming nonbonded area,
the first flexible substrate comprises a through-hole that is in contact with the flow channel-forming non-bonded area,
a shutter-forming non-bonded area is formed on a bonding surface of the second flexible substrate and the third substrate in a band-like manner at a far side of the through-hole relative to the extraction chamber-forming non-bonded area such that the shutter-forming non-bonded area and the flow channel-forming non-bonded area intersect via the second flexible substrate being interposed therebetween,
at least one of both of the first flexible substrate and the second flexible substrate and the third substrate comprises a pressure supply port that comes through the substrate(s) so as to be in contact with the shutter-forming non-bonded area, a magnetic particle that binds to a target substance is placed above the extraction chamber-forming non-bonded area,
a flow channel and an extraction chamber are formable by supplying pressure from the through-hole to raise a site above the flow channel-forming non-bonded area and a site above the extraction chamber-forming non-bonded area,
the flow channel is blockable by supplying pressure from the pressure supply port to raise a site above the shutter-forming non-bonded area,
the magnetic field generating unit is disposed 1) at an outer side of at least one of the first flexible substrate or the third flexible substrate, and 2) in a vicinity of an end of the extraction chamber at the opposite side of the through-hole, and
the target substance that is bound to the magnetic particle is capturable by the magnetic field at at least one of an undersurface of the third substrate directly below an end of the extraction chamber at the opposite side of the through-hole or a top surface of the first flexible substrate directly above an end of the extraction chamber at the opposite side of the through-hole.

4. The system according to claim 3 , wherein the chip further comprises:
a PCR amplification portion; and
an electrophoresis analysis portion.

Assignees

• Nec Corp

Inventors

• Asogawa Minoru
• Hagiwara Hisashi
• Mishina Yoshinori
• Iimura Yasuo

Classifications

• C12Q1/686 Primary

  Polymerase chain reaction [PCR] · CPC title

• B01L2300/0861

  Configuration of multiple channels and/or chambers in a single devices · CPC title

• B01L2400/043

  magnetic forces · CPC title

• B01L2300/0864

  comprising only one inlet and multiple receiving wells, e.g. for separation, splitting · CPC title

• G01N27/453

  Cells therefor · CPC title