Multisignal labeling reagents and processes and uses therefor

Patent metadata

Field	Value
Publication number	US-9884889-B2
Application number	US-201514844468-A
Country	US
Kind code	B2
Filing date	Sep 3, 2015
Priority date	Apr 3, 2003
Publication date	Feb 6, 2018
Grant date	Feb 6, 2018

Abstract

Provided are compounds comprising two DNA supramolecular binding molecules covalently joined by a linker group. Also provided are multisignal labeling reagents comprising (i) an oligomer of nucleotides or nucleotide analogs; (ii) a DNA supramolecular binding molecule noncovalently bound to the oligomer; and (iii) a first reactive group or a first partner of a first binding pair covalently bound to the oligomer. Additionally provided are methods of producing multisignal labeling reagents.

First claim

What is claimed is:

1. A multisignal labeling reagent comprising:
(i) an oligomer of nucleotides or nucleotide analogs;
(ii) a plurality of DNA supramolecular binding molecules noncovalently bound to the oligomer,
wherein said DNA supramolecular binding molecules comprise a detectable label attached thereto that is not a DNA supramolecular binding molecule, and
wherein said DNA supramolecular binding molecules are selected from minor groove binders and major groove binders; and

(iii) a first reactive group covalently bound to the oligomer, or
a first partner of a first binding pair covalently bound to the oligomer.

2. The multisignal labeling reagent of claim 1 , wherein the DNA supramolecular binding molecule is fluorescent.

3. The multisignal labeling reagent of claim 1 , wherein the detectable label is non-radioactive.

4. The multisignal labeling reagent of claim 3 , wherein the non-radioactive detectable label is a fluorophore.

5. The multisignal labeling reagent of claim 3 , wherein the non-radioactive detectable label is a chemiluminescent or phosphorescent moiety.

6. The multisignal labeling reagent of claim 1 , wherein the DNA supramolecular binding molecules comprise DNA supramolecular binding molecules which are minor groove binders.

7. The multisignal labeling reagent of claim 6 , wherein the minor groove binders comprise a minor groove binder selected from the group consisting of a diacrylamidine and a bis-benzimidazole.

8. The multisignal labeling reagent of claim 6 , wherein the minor groove binders comprise a minor groove binder selected from the group consisting of DAPI, berenil, pentamidine, distamycin A, and Hoechst 33258.

9. The multisignal labeling reagent of claim 1 , wherein the DNA supramolecular binding molecules comprise DNA supramolecular binding molecules which are major groove binders.

10. The multisignal labeling reagent of claim 9 , wherein the major groove binders comprise a major groove binder selected from the group consisting of a zinc finger, a leucine zipper, and a helix-turn-helix motif.

11. The multisignal labeling reagent of claim 1 , comprising the first partner of the first binding pair covalently bound to the oligomer.

12. The multisignal labeling reagent of claim 11 , wherein the first binding pair is a ligand/receptor, a hormone/receptor, biotin/avidin, biotin/streptavidin or an antigen/antibody binding pair.

13. The multisignal labeling reagent of claim 11 , wherein the first partner of the first binding pair is streptavidin.

14. The multisignal labeling reagent of claim 1 , comprising the first reactive group covalently bound to the oligomer.

Assignees

• Enzo Life Science Inc
• Enzo Life Sciences Inc

Inventors

• Coleman Jack
• Rabbani Elazar
• Stavrianopoulos Jannis
• Pande Praveen

Classifications

• C09B57/02

  Coumarine dyes · CPC title

• C07H21/00 Primary

  Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids · CPC title

• C07D491/22

  in which the condensed system contains four or more hetero rings · CPC title

• G01N33/582

  with fluorescent label · CPC title

• C07D219/08

  Nitrogen atoms · CPC title