Anti-dengue virus antibodies and uses thereof

Patent metadata

Field	Value
Publication number	US-9880167-B2
Application number	US-201615291199-A
Country	US
Kind code	B2
Filing date	Oct 12, 2016
Priority date	Aug 7, 2012
Publication date	Jan 30, 2018
Grant date	Jan 30, 2018

Abstract

The present invention provides, among other things, antibody agents (e.g., antibodies, and/or antigen-binding fragments thereof) that bind to DV epitopes, as well as compositions containing them and methods of designing, providing, formulating, using, identifying and/or characterizing them. In some embodiments, provided antibody agents show significant binding to a plurality of DV serotypes. In some embodiments, provided antibody agents show significant binding to all four DV serotypes. Such antibody agents are useful, for example, in the prophylaxis, treatment, diagnosis, and/or study of DV.

First claim

What is claimed is:

1. A method of detecting Dengue virus, the method comprising steps of:
contacting a sample with an antibody agent specific to Dengue virus,
which antibody agent comprises a heavy chain variable region and a light chain variable region, each of which includes complementarity determining regions (CDRs), wherein
the heavy chain variable region comprises CDRs whose amino acid sequences are set forth in SEQ ID NO: 23, SEQ ID NO: 24, and SEQ ID NO: 25;
the light chain variable region comprises CDRs whose amino acid sequences are set forth in SEQ ID NO: 26, SEQ ID NO: 27, and SEQ ID NO: 28; and
detecting binding of the antibody agent to the sample.

2. The method of claim 1 , wherein the sample is selected from the group consisting of clinical, environmental, and research samples.

3. The method of claim 1 , wherein the sample is or comprises one or more of blood, serum/plasma, peripheral blood mononuclear cells/peripheral blood lymphocytes (PBMC/PBL), sputum, urine, feces, throat swabs, dermal lesion swabs, cerebrospinal fluids, cervical smears, pus samples, food matrices, and tissues from various parts of the body including brain, spleen, and liver.

4. The method of claim 1 , wherein the sample is or comprises one or more of soil, water, and flora.

Assignees

• Massachusetts Inst Technology

Inventors

• Sasisekharan Ram
• Robinson Luke Nathaniel
• Tharakaraman Kannan

Classifications

• C07K16/116 Primary

  Togaviridae (F); Matonaviridae (F); Flaviviridae (F) · CPC title

• C07K2317/565

  Complementarity determining region [CDR] · CPC title

• C07K2317/34

  Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues · CPC title

• C07K2317/33

  Crossreactivity, e.g. for species or epitope, or lack of said crossreactivity · CPC title

• C07K2317/76

  Antagonist effect on antigen, e.g. neutralization or inhibition of binding · CPC title