Non-viral episomal suicide construct

Patent metadata

Field	Value
Publication number	US-9879278-B2
Application number	US-201314382816-A
Country	US
Kind code	B2
Filing date	Feb 27, 2013
Priority date	Mar 5, 2012
Publication date	Jan 30, 2018
Grant date	Jan 30, 2018

Abstract

The invention includes compositions and methods for the selective expression of a target gene in a subset of cells. In certain embodiments, the present invention includes a construct comprising a first nucleic acid sequence comprising an episomal maintenance element and a second nucleic acid sequence comprising a target gene wherein the expression of the episomal maintenance element is regulated by a constitutive promoter and the expression of the target gene is regulated by a non-constitutive promoter. The construct is able to maintain episomal state, no matter whether the target gene is expressed in the cell.

First claim

What is claimed is:

1. A vector comprising a first nucleic acid comprising a scaffold/matrix attached regions (S/MAR) episomal maintenance element sequence, and a second nucleic acid comprising a suicide gene,
wherein the first nucleic acid comprises in 5′-3′ order, a constitutive promoter, a gene operably linked to the constitutive promoter, an (S/MAR) sequence and a polyadenylation signal, wherein transcription of the gene operably linked to the constitutive promoter, together with the S/MAR sequence and the polyadenylation signal, is controlled by the constitutive promoter,
wherein the second nucleic acid comprises an undifferentiated stem cell specific promoter operably linked to the suicide gene, and wherein expression of the suicide gene is controlled by the undifferentiated stem cell specific promoter, further wherein when the suicide gene is expressed in an undifferentiated stem cell, the undifferentiated stem cell is susceptible to cell death, and
wherein the undifferentiated stem cell specific promoter is selected from the group consisting of OCT4, hTERT, and NANOG, and the suicide gene encodes thymidine kinase (TK).

2. The vector of claim 1 , wherein the S/MAR episomal maintenance element sequence is capable of long term episomal maintenance of the vector in an entire cell population, and wherein the non-constitutive promoter is capable of expression of the suicide gene in a subset of cells of the entire cell population.

3. The vector of claim 1 , wherein the gene operatively linked to the constitutive promoter is Kan/Neo.

4. The vector of claim 1 , wherein the undifferentiated stem cell specific promoter is the promoter of OCT4 gene.

Assignees

• Univ Wake Forest Health Sciences

Inventors

• Lu Baisong
• Zhao Qingguo
• Yoo James
• Atala Anthony

Classifications

• C12N2800/40

  Systems of functionally co-operating vectors · CPC title

• C12N15/85 Primary

  for animal cells · CPC title

• C12N2840/007

  cell or tissue specific · CPC title