Thermophilic and thermoacidophilic metabolism genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods

Patent metadata

Field	Value
Publication number	US-9879247-B2
Application number	US-201615347903-A
Country	US
Kind code	B2
Filing date	Nov 10, 2016
Priority date	Feb 28, 2008
Publication date	Jan 30, 2018
Grant date	Jan 30, 2018

Abstract

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering metabolism in a cell using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

First claim

What is claimed is:

1. An expression vector comprising an isolated polynucleotide encoding a polypeptide having at least 90% sequence identity to SEQ ID No. 443 and a nucleotide sequence heterologous to the polynucleotide.

2. The expression vector of claim 1 , wherein the expression vector comprises an isolated polynucleotide having at least 95% identity to SEQ ID No. 444.

3. The expression vector of claim 1 , wherein the encoded polypeptide has 3-hydroxybutyryl-CoA dehydratase enzymatic activity.

4. A method of modulating or altering metabolism in a cell, the method comprising:
providing the expression vector of claim 3 to the cell and expressing the encoded polypeptide in the cell.

5. The method according to claim 4 , further comprising glycosylating, or otherwise post-translationally modifying the encoded peptide in the cell.

6. An expression vector comprising an isolated polynucleotide encoding a polypeptide having at least 95% sequence identity to SEQ ID No. 443 and a nucleotide sequence heterologous to the polynucleotide.

7. The expression vector of claim 6 , wherein the encoded polypeptide has 3-hydroxybutvrvl-CoA dehydratase enzymatic activity.

8. The expression vector of claim 6 , wherein the expression vector comprises an isolated polynucleotide having at least 95% identity to SEQ ID No. 444.

Assignees

• Battelle Energy Alliance Llc

Inventors

• Thompson Vicki S
• Apel William A
• Lacey Jeffrey A
• Lee Brady D
• Reed David William
• Roberto Francisco F
• Thompson David N

Classifications

• C12N15/74

  Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora · CPC title

• C12Y402/01055

  3-Hydroxybutyryl-CoA dehydratase (4.2.1.55) · CPC title

• C12Y203/01009

  Acetyl-CoA C-acetyltransferase (2.3.1.9) · CPC title

• C12N9/00

  Enzymes; Proenzymes; Compositions thereof (preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; {enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113}); Processes for preparing, activating, inhibiting, separating or purifying enzymes (preparation of malt C12C1/00) · CPC title

• C12N9/1029

  transferring groups other than amino-acyl groups (2.3.1) · CPC title