Fluid impeller for bioprocessing

Patent metadata

Field	Value
Publication number	US-9878295-B2
Application number	US-201514684705-A
Country	US
Kind code	B2
Filing date	Apr 13, 2015
Priority date	Apr 13, 2015
Publication date	Jan 30, 2018
Grant date	Jan 30, 2018

Abstract

Fluid impellers for biocontainers, and bioprocessing units including the impellers and biocontainers, and methods of using the impellers, are disclosed.

First claim

The invention claimed is:

1. A bioprocessing unit for use in bioprocessing comprising:
(a) a biocontainer comprising a closed container having an interior volume suitable for containing fluid, the container comprising a bottom wall, a top wall, at least one side wall, the at least one side wall being joined to the top wall and the bottom wall; and at least an inlet port, and a drain port, the drain port being arranged in the bottom wall, wherein the biocontainer further comprises:
(b) a rotatable agitator comprising
(i) a rotatable shaft comprising a cylindrical element having a first end and a second end, the shaft having a vertical rotational axis, the shaft passing through the bottom wall of the biocontainer such that the second end extends exterior to the bottom wall;
(ii) an impeller comprising a hub mounted on the first end of the rotatable shaft, the hub including a top end and a bottom end and at least first and second impeller arm slots arranged vertically and extending from the top end toward the bottom end;
(iii) at least first, second, and third impeller blades extending from the hub, each of the first, second, and third impeller blades comprising a blade face, an arm having a first end and a second end and a notch between the first end and the second end, wherein the first end is adjacent to the blade face, and the second end extends into an impeller slot, the arm being engaged in the slot, wherein the second end of the first impeller blade arm engages with the notch of the third impeller blade arm, the second end of the third impeller blade arm engages with the notch of the second impeller blade arm, and the second end of the second impeller blade arm engages with the notch of the first impeller blade arm; and
(iv) a housing assembly coupled to the bottom wall of the biocontainer, the housing assembly sealingly supporting the rotatable shaft, wherein the second end of the shaft passes through the housing assembly.

2. The bioprocessing unit of claim 1 , wherein the impeller comprises rivets or bolts fixing each arm to the hub.

3. The bioprocessing unit of claim 1 , further comprising a hub cap engaged with the top end of the hub.

4. A method for preparing a mixed fluid used in bioprocessing comprising
passing at least one fluid and at least one component to be mixed with the fluid through at least one port into a biocontainer of a bioprocessing unit, the bioprocessing unit comprising:
(a) the biocontainer, which comprises a closed container having an interior volume suitable for containing fluid, the container comprising a bottom wall, a top wall, at least one side wall, the at least one side wall being joined to the top wall and the bottom wall; and at least an inlet port, and a drain port, the drain port being arranged in the bottom wall, wherein the biocontainer further comprises:
(b) a rotatable agitator comprising
(i) a rotatable shaft comprising a cylindrical element having a first end and a second end, the shaft having a vertical rotational axis, the shaft passing through the bottom wall of the biocontainer such that the second end extends exterior to the bottom wall;
(ii) an impeller comprising a hub mounted on the first end of the rotatable shaft, the hub including a top end and a bottom end and at least first and second impeller arm slots arranged vertically and extending from the top end toward the bottom end;
(iii) at least first, second, and third impeller blades extending from the hub, each of the first, second, and third impeller blades comprising a blade face, an arm having a first end and a second end and a notch between the first end and the second end, wherein the first end is adjacent to the blade face, and the second end extends into an impeller slot, the arm being engaged in the slot, wherein the second end of the first impeller blade arm engages with the notch of the third impeller blade arm, the second end of the third impeller blade arm engages with the notch of the second impeller blade arm, and the second end of the second impeller blade arm engages with the notch of the first impeller blade arm; and
(iv) a housing assembly coupled to the bottom wall of the biocontainer, the housing assembly sealingly supporting the rotatable shaft, wherein the second end of the shaft passes through the housing assembly; and

rotating the impeller, and mixing the at least one fluid and the at least one component to be mixed with the fluid, and producing the mixed fluid.

Assignees

• Pall Corp

Inventors

• Marshall Glenn P

Classifications

• C12M27/02 Primary

  Stirrer or mobile mixing elements · CPC title

• C12M23/14

  Bags · CPC title

• C12M27/18

  Flow directing inserts · CPC title

• B01F7/22

  Operations & Transport · mapped topic

• B01F7/162

  Operations & Transport · mapped topic