Methods of growing plants using modified biological control agents

Patent metadata

Field	Value
Publication number	US-9877486-B2
Application number	US-201514808473-A
Country	US
Kind code	B2
Filing date	Jul 24, 2015
Priority date	Jan 31, 2014
Publication date	Jan 30, 2018
Grant date	Jan 30, 2018

Abstract

Methods for improving the ability of a population of biological agents to compete and survive in a field setting are provided. By improving the population of biological agents, the modified population of agents is able to grow, compete with other microbial strains and fungi, and provide protection for plants from pathogens. In particular, modified biological agents and modified populations of such agents that are herbicide tolerant or resistant are selected or engineered. In this manner, the protection from disease-causing agents is enhanced. Such modified populations of biological agents can be added to soils to prevent fungal pathogens and the diseases they cause promoting plant growth. Therefore, the present invention is useful for enhancing the competitiveness of modified biological agents particularly over other microbial agents which are not herbicide resistant. Compositions of the invention include selected or engineered herbicide resistant biological agents and modified populations of biocontrol agents. These modified biological agents can be used as an inoculant or as a seed coating for plants and seeds.

First claim

What is claimed is:

1. A method for growing a plant comprising planting in an area of cultivation a coated seed composition comprising a seed and a coating on said seed, the coating comprising a biological control agent, wherein the biological control agent is deposited as NRRL No. B-50897 and is a viable Pseudomonas chlororaphis strain, and wherein the biological control agent is provided on said seed at about 10 4 CFU/seed to about 10 8 CFU/seed.

2. The method of claim 1 , wherein said coating further comprises at least one of a nutrient, a herbicide, a fungicide, an insecticide, or a pesticide.

3. The method of claim 1 , wherein said coating controls a plant pathogen.

4. The method of claim 1 , wherein the pathogen is a fungus.

5. The method of claim 1 , wherein the coating on the seed controls at least one of Pythium aphanidermatum, Phytophthora parasitica, Rhizoctonia solani , and/or Botrytis cinerea.

6. The method of claim 1 , wherein said seed is from a monocot.

7. The method of claim 1 , wherein said seed is from a dicot.

8. The method of claim 7 , wherein said dicot is a soybean.

9. The method of claim 1 , wherein the biological control agent is present on said seed at about 10 5 CFU/seed to about 10 7 CFU/seed.

Assignees

• Agbiome Inc

Inventors

• Hammer Philip E
• Jones Janice C
• Koziel Michael G
• Smith Kelly S
• Uknes Scott Joseph

Classifications

• C12N15/78

  for Pseudomonas · CPC title

• C12Y205/01019

  3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase · CPC title

• C12N9/1092

  3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase · CPC title

• A01N43/40

  six-membered rings · CPC title

• A01N37/22

  the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides · CPC title