Facile method for making non-toxic biomedical compositions comprising hybrid metal-polymer microparticles

Patent metadata

Field	Value
Publication number	US-9872916-B2
Application number	US-201314390945-A
Country	US
Kind code	B2
Filing date	Apr 5, 2013
Priority date	Apr 6, 2012
Publication date	Jan 23, 2018
Grant date	Jan 23, 2018

Abstract

The present invention includes photochemical method of making hybrid metal-polymer microparticles in an aqueous, biocompatible solution by providing a metal (I) composition and one or more polymeric materials; applying an electromagnetic radiation to the metal (I) composition; converting the metal (I) composition to a metal (0) composition; forming one or more hybrid metal-polymer microparticles from the metal (0); capping the one or more hybrid metal-polymer microparticles; and stabilizing the one or more hybrid metal-polymer microparticles with the one or more polymeric materials to prevent agglomeration.

First claim

The invention claimed is:

1. A method of making a plasmonic hybrid metal-polymer microparticle complex comprising the steps of:
forming a hybrid metal-polymer microparticle with a tunable near-infrared (NIR) plasmon absorption in an aqueous, biocompatible solution by
providing an aqueous biocompatible solution;
combining a metal (I) composition and one or more polymeric materials in the aqueous biocompatible solution;
applying an electromagnetic radiation for 30-40 minutes, to the metal (I) composition to convert the metal (I) composition to a metal (0) composition;
forming one or more hybrid metal-polymer microparticles from the metal (0);
capping the one or more hybrid metal-polymer microparticles; and

stabilizing the one or more hybrid metal-polymer microparticles with the one or more polymeric materials to prevent agglomeration and to provide the NIR plasmon absorption of the one or more hybrid metal-polymer microparticles between 700 nm-1200 nm; and
forming one or more stabilized hybrid metal-polymer microparticles.

2. The method of claim 1 , further comprising the step of adding one or more binding agents to the one or more hybrid metal-polymer microparticles to form a diagnosis complex that binds to a specific agent, wherein the one or more binding agents comprise one or more antibodies or oligonucleotides.

3. The method of claim 1 , further comprising the step of adding one or more agents that enhance Raman scattering and identifying one or more small biological molecules using surface enhanced Raman scattering.

4. The method of claim 1 , further comprising the step of adding an active agent and a pharmaceutical carrier to form a pharmaceutical plasmonic hybrid metal-polymer microparticle composition for delivery of an active agent.

5. The method of claim 1 , wherein the metal (0) comprises at least one metal atom selected from the group consisting of aluminum, antimony, arsenic, barium, beryllium, bismuth, cadmium, calcium, cerium, chromium, cobalt, dysprosium, erbium, europium, gadolinium, gallium, hafnium, holmium, indium, iridium, iron, lanthanum, lead, lithium, lutetium, magnesium, manganese, mercury, molybdenum, neodymium, nickel, niobium, osmium, palladium, platinum, potassium, praseodymium, rhenium, rhodium, rubidium, ruthenium, samarium, scandium, strontium, tantalum, technetium, terbium, titanium, thallium, thorium, thulium, tin, tungsten, uranium, vanadium, ytterbium, yttrium, zinc, and zirconium.

6. The method of claim 1 , wherein the metal (I) composition comprises a gold (I) complex, silver (I) complex or salt, copper (I) complex or salt, or combinations thereof.

7. The method of claim 1 , wherein the metal (I) composition is made by adding a metal (I) precursor comprising Au(tetrahydrothiophene)Cl, AuMe 2 SCl, Au(CO)Cl, AgNO 3 or AgPF 6 or Cu(PPh 3 ) 3 X complexes.

8. The method of claim 1 , wherein the one or more polymer stabilizers comprise agarose, hydrogels, PAA (poly acrylic acid), PVA (poly vinyl alcohol), Chitosan, PNIPAM (Poly-N-isopropyl acrylamide), substituted PNIPAM, PAMAM (Polyamidoamine), PEG (Poly ethylene glycol), alginic acid, HPC (hydroxyl propyl cellulose), a crude phospholipid extract isolated from soybeans or a combination thereof.

9. The method of claim 1 , wherein the one or more polymeric materials comprise Chitosan, Polyacrylic acid, Alginic acid, PEG, PVA, Agarose, albumin, bovine serum albumin (BSA), human albumin, synthetic albumin, HPC, PNIPAM, Nylon, polyurethane, polyacrylonitrile or a crude phospholipid extract isolated from soybeans.

10. A method of making a plasmonic hybrid metal-polymer microparticle complex comprising the steps of:
forming a hybrid metal-polymer microparticle with a tunable near-infrared (NIR) plasmon absorption in an aqueous, biocompatible solution by
providing an aqueous biocompatible solution;
combining a metal (I) composition wherein the metal (I) is silver and one or more polymeric materials in the aqueous biocompatible solution wherein one or more polymeric materials comprise Nylon, polyurethane, or polyacrylonitrile;
applying an electromagnetic radiation for 30-40 minutes to the metal (I) composition to convert the metal (I) composition to a metal (0) composition;
forming one or more hybrid metal-polymer microparticles from the metal (0);
capping the one or more hybrid metal-polymer microparticles; and

stabilizing the one or more hybrid metal-polymer microparticles with the one or more polymeric materials to prevent agglomeration and to provide the NIR plasmon absorption of the one or more hybrid metal-polymer microparticles between 700 nm-1200 nm; and
forming one or more stabilized hybrid metal-polymer microparticles.

11. The method of claim 1 , wherein the hybrid metal-polymer microparticle composition is varied in size, shape or both.

12. The method of claim 1 , further comprising the step adjusting one or more parameters selected from the group consisting of pH, ionic strength, temperature, centrifugation, reaction vessel material, optical filters, and combinations thereof, to adjust at least one of the tuning of the plasmon absorption energies or intensities and corresponding variation of at least one of size or shape of the one or more hybrid metal-polymer microparticles to adjust a plasmon absorption energy, an intensity or a combination thereof.

13. The method of claim 1 , wherein the hybrid metal-polymer microparticle composition is varied in NIR plasmon absorption by varying a time of exposure and concentrations of the one or more polymeric materials and the metal (I) composition.

14. The method of claim 1 , wherein the electromagnetic radiation is in the form of UV light, Sunlight, microwave radiation, far infrared radiation, near infrared radiation, visible radiation, x-rays, gamma rays, or high-energy gamma rays.

15. The method of claim 4 , wherein the active agent is an agent that binds an antibody, a cell surface, or a small biological molecule.

16. The method of claim 4 , wherein the active agent is delivered by a phase transition change in the pharmaceutical plasmonic hybrid metal-polymer microparticle composition; by a phase transition in the hybrid metal-polymer microparticle resulting from an electromagnetic change; or by a phase transition in the hybrid metal-polymer microparticle resulting from a thermal change.

17. A hybrid metal-polymer microparticle composite made by:
providing an aqueous biocompatible solution;
combining a metal (I) composition and one or more polymeric materials and one or more nanofibers;
applying an electromagnetic radiation for 30-40 minutes to the metal (I) composition;
converting the metal (I) composition to a metal (0) composition;
forming one or more hybrid metal-polymer microparticles from the metal (0);
capping the one or more hybrid metal-polymer microparticles;
stabilizing the one or more hybrid metal-polymer microparticles with the one or more polymeric materials to prevent agglomeration;
forming a hybrid metal-polymer microparticle composite; and
tuning of a near-infrared (NIR) plasmon absorption of the one or more hybrid metal-polymer microparticles between 700 nm-1200 nm by varying time of exposure, concentrations of the one or more polymeric materials, concentrations of the metal (I) composition or a combination thereof.

18. The hybrid metal-polymer microparticle composite of claim 17 , wherein the metal (I) composition is silver to form an antipathogenic biocompatible polymer composition.

19. The hybrid metal-polymer microparticle composite of claim 17 , wherein the o

Assignees

• Univ North Texas
• Univ Of North Texas Office Of Economic Development And Technology Transfer

Inventors

• Omary Mohammad A
• Marpu Sreekar

Classifications

• B22F1/102

  Metallic powder coated with organic material · CPC title

• A61K9/501

  Inorganic compounds · CPC title

• B22F1/0018

  Operations & Transport · mapped topic

• Y10T436/25

  including sample preparation · CPC title

• B82Y30/00

  Nanotechnology for materials or surface science, e.g. nanocomposites · CPC title