Collagen-based matrices with stem cells

Patent metadata

Field	Value
Publication number	US-9867905-B2
Application number	US-33208408-A
Country	US
Kind code	B2
Filing date	Dec 10, 2008
Priority date	Dec 10, 2007
Publication date	Jan 16, 2018
Grant date	Jan 16, 2018

Abstract

Collagen based-matrices and methods of their use are described. More particularly, collagen-based matrices for differentiating stem cells and progenitor cells, and for producing and isolating blood vessels and vascularized graft constructs are described.

First claim

What is claimed is:

1. A tissue graft composition, said composition comprising:
an engineered, purified collagen-based matrix comprising collagen fibrils, wherein the matrix has a collagen fibril microstructure including fibril-fibril branching; and
a network of lumenized, endothelial cell-lined vessels formed in vitro, said vessels capable of persisting for more than 7 days in vitro;
wherein said collagen fibrils are polymerized from a pig skin collagen source comprising a mixture of type I collagen monomers and oligomers, wherein the concentration of collagen in the polymerization reaction is 0.3 mg/ml to 1 mg/ml, wherein the vessels are formed from endothelial progenitor cells, and wherein the matrix has a shear storage modulus of 40 Pa to 50 Pa and a fibril volume fraction of 5% to 10%; and
wherein the tissue graft composition is produced by engineering the purified collagen-based matrix, and seeding the matrix at a density of 1×10 5 to 5×10 5 endothelial progenitor cells per milliliter to form one or more vessels within the matrix.

2. The tissue graft composition of claim 1 , wherein said matrix has a loss modulus of from about 1 Pa to about 75 Pa.

3. The tissue graft composition of claim 1 , wherein said matrix has a compression modulus of 2,500 Pa to about 18,000 Pa.

Assignees

• Voytik-Harbin Sherry L
• Kreger Seth
• Yoder Mervin C
• Critser Paul
• Purdue Research Foundation
• Univ Indiana Res & Tech Corp

Inventors

• Voytik-Harbin Sherry L
• Kreger Seth
• Yoder Mervin C
• Critser Paul

Classifications

• A61L27/507 Primary

  for artificial blood vessels (apparatus for applying cells on a blood vessel prosthesis A61F2/062) · CPC title

• A61L27/24 Primary

  Collagen · CPC title

• A61L27/3808

  Endothelial cells · CPC title

• A61L27/3834

  Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells · CPC title

• C12N5/0623

  Stem cells · CPC title