Filtration device for rapid separation of biological particles from complex matrices

Patent metadata

Field	Value
Publication number	US-9861939-B2
Application number	US-201514874326-A
Country	US
Kind code	B2
Filing date	Oct 2, 2015
Priority date	Oct 2, 2015
Publication date	Jan 9, 2018
Grant date	Jan 9, 2018

Abstract

Methods and systems for filtering of biological particles are disclosed. Filtering membranes separate adjacent chambers. Through osmotic or electrokinetic processes, flow of particles is carried out through the filtering membranes. Cells, viruses and cell waste can be filtered depending on the size of the pores of the membrane. A polymer brush can be applied to a surface of the membrane to enhance filtering and prevent fouling.

First claim

What is claimed is:

1. A device comprising:
a plurality of chambers;
at least one filtering membrane between a first chamber and a second chamber of the plurality of chambers, the at least one filtering membrane having a pore size based on a desired biological particle to be filtered;
at least one polymer brush layer, attached to the at least one filtering membrane on a side downstream to a fluidic flow between the first chamber and the second chamber; and
magnetic nanoparticles in at least one chamber of the plurality of chambers,
wherein the magnetic nanoparticles comprise a polymer brush layer on their surfaces.

2. The device of claim 1 , wherein the plurality of chambers comprises three chambers, a first filtering membrane between the first chamber and the second chamber, and a second filtering membrane between the second and a third chamber.

3. The device of claim 1 , further comprising a first electrode in the first chamber and a second electrode in the second chamber, to provide fluidic flow by electrokinetic forces.

4. The device of claim 1 , wherein the desired biological particle is a virus, a bacterium, cell, or cell waste.

5. The device of claim 1 , further comprising two electrodes configured to apply an electrostatic potential to the plurality of chambers, thereby driving the fluidic flow by electrokinetic forces.

6. The device of claim 1 , wherein the at least one polymer brush layer is made of polyethylene glycol.

7. The device of claim 1 , further comprising a hydrogel polymer in at least one chamber of the plurality of chambers, the hydrogel polymer configured to apply osmotic pressure to the plurality of chambers.

8. The device of claim 7 , wherein the hydrogel polymer is a hydrogel polymer scaffold.

9. A method comprising:
providing a plurality of chambers, at least one filtering membrane between a first chamber and a second chamber of the plurality of chambers, the at least one filtering membrane having a pore size based on a desired biological particle to be filtered, at least one polymer brush layer, attached to the at least one filtering membrane on a side downstream to a fluidic flow between the first chamber and the second chamber;
inserting a solution containing biological particles in the first chamber of the plurality of chambers;
driving the fluidic flow through the plurality of chambers; and
extracting the desired biological particle after filtering through the plurality of chambers,
wherein:
the plurality of chambers further comprises magnetic nanoparticles in at least one chamber of the plurality of chambers, and
the magnetic nanoparticles comprise a polymer brush layer on their surfaces.

10. The method of claim 9 , wherein the plurality of chambers further comprises at least two electrodes, the method further comprising:
applying an electrostatic field to the plurality of chambers by the at least two electrodes, thereby driving the fluidic flow by electrokinetic forces.

11. The method of claim 9 , wherein the plurality of chambers further comprises a hydrogel polymer in at least one chamber of the plurality of chambers, the method further comprising:
applying the osmotic pressure by the hydrogel polymer.

12. The method of claim 11 , wherein the hydrogel polymer is a hydrogel polymer scaffold.

13. The method of claim 9 , further comprising applying a magnetic field in at least one chamber of the plurality of chambers.

14. The method of claim 13 , wherein applying a magnetic field comprises controlling a flow of the magnetic nanoparticles.

15. The method of claim 14 , wherein controlling the flow of the magnetic nanoparticles comprises separating the magnetic nanoparticles from the biological particles.

Assignees

• L Livermore Nat Security Llc

Inventors

• Kim Sangil
• Naraghi-Arani Pejman
• Liou Megan

Classifications

• B01D61/005 Primary

  Osmotic agents; Draw solutions · CPC title

• C12N13/00

  Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title

• B01D65/02 Primary

  Membrane cleaning or sterilisation {; Membrane regeneration} · CPC title

• B01D2317/02

  Elements in series · CPC title

• B01D61/427

  Electro-osmosis · CPC title