Replication-competent vesicular stomatitis viruses

Patent metadata

Field	Value
Publication number	US-9861668-B2
Application number	US-201615380728-A
Country	US
Kind code	B2
Filing date	Dec 15, 2016
Priority date	Apr 18, 2012
Publication date	Jan 9, 2018
Grant date	Jan 9, 2018

Abstract

This document provides methods and materials related to vesicular stomatitis viruses. For example, replication-competent vesicular stomatitis viruses, nucleic acid molecules encoding replication-competent vesicular stomatitis viruses, methods for making replication-competent vesicular stomatitis viruses, and methods for using replication-competent vesicular stomatitis viruses to treat cancer or infectious diseases are provided.

First claim

What is claimed is:

1. A replication-competent vesicular stomatitis virus comprising an RNA molecule, wherein said RNA molecule comprises a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV N polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV P polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV M polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding a measles virus F polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding a measles virus H polypeptide, and a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV L polypeptide, wherein said RNA molecule lacks a nucleic acid sequence that is a template for a positive sense transcript encoding a functional VSV G polypeptide.

2. The virus of claim 1 , wherein said Measles H polypeptide is a measles virus H polypeptide comprising Y481A and R533A amino acid substitutions with respect to a wild-type measles virus H polypeptide.

3. The virus of claim 1 , wherein said Measles H polypeptide comprises an amino acid sequence of a single chain antibody.

4. The virus of claim 3 , wherein said single chain antibody is a single chain antibody directed to EGFR, αFR, or PSMA.

5. The virus of claim 1 , wherein said RNA molecule virus comprises a nucleic acid sequence that is a template for a positive sense transcript encoding a NIS polypeptide.

Assignees

• Mayo Found Medical Education & Res

Inventors

• Peng Kah-Whye
• Russell Stephen James
• Breton Camilo Ayala

Classifications

• C12N2760/20271

  Demonstrated in vivo effect · CPC title

• C12N2760/18422

  New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title

• C12N2810/6081

  rhabdoviridae, e.g. VSV · CPC title

• C12N2810/859

  from immunoglobulins · CPC title