Specificity, flexibility and valence of DNA bonds for guided emulsion architecture

Patent metadata

Field	Value
Publication number	US-9861569-B2
Application number	US-201414266026-A
Country	US
Kind code	B2
Filing date	Apr 30, 2014
Priority date	May 1, 2013
Publication date	Jan 9, 2018
Grant date	Jan 9, 2018

Abstract

A method of forming an end product by self-assembly of a first component having a patch of a linker component, such as DNA strands, cadherins, adhesive proteins and nanoparticle linkers. Such emulsions can be used to process personal care products, skin cremes, foods and animal feedstocks.

First claim

What is claimed is:

1. A method of self-assembly to form end product, comprising the steps of:
providing a first emulsion droplet for assembly;
providing a second emulsion droplet for assembly;
providing a first linker comprising a colloidal nanoparticle which can be coupled to the first emulsion droplet;
forming a first patch, comprising the first linker, between the first emulsion droplet and the second emulsion droplet.

2. The method as defined in claim 1 wherein pseudo-bonding valency is established for the linker components.

3. The method as defined in claim 2 wherein a valency of 2 enables formation of flexible polymers of emulsion droplets.

4. The method as defined in claim 2 wherein a pseudo-valency of 4 enables formation of rigid polymer networks.

5. The method as defined in claim 1 wherein the first linker further comprises a plurality of single stranded DNA, attached to the colloidal nanoparticle, having a first sequence and further wherein the first emulsion droplet and the second emulsion droplet each have attached thereto a plurality of single stranded DNA having a second sequence, the second sequence and the first sequence being complementary.

6. The method as defined in claim 1 further including the step of mixing the end production with a personal care product matrix.

7. The method as defined in claim 1 further including the step of mixing the end product with a food product matrix.

8. The method as defined in claim 1 wherein the end production consists of an amorphous material having selectable rheological properties, thereby enabling processing of the end product to operate consumer products having desired properties.

9. The method as defined in claim 1 further including the step of cycling temperature of the end product.

10. The method as defined in claim 1 wherein the linker component comprises DNA strands further including the step of increased coverage of the DNA strands on the first component, thereby enabling tuning of reversibility of self-assembly.

11. A method of self-assembly to form end product, comprising the steps of:
providing an emulsion having a plurality of emulsion droplets and a plurality of linkers, wherein each linker comprises a nanoparticle having attached thereto a first DNA strand and a second DNA strand and the concentration of the plurality of linkers is selected to provide a desired valency;
providing a first linker and a second linker of the plurality of linkers, each of the first linker and the second linker engagable with the plurality of emulsion droplets and engageable with each other;
engaging the first DNA strand with a first emulsion droplet of the plurality of droplets;
engaging the second DNA strand with a second emulsion droplet of the plurality of droplets;
forming a patch between the first emulsion droplet and the second emulsion droplet comprising the first DNA strand, the nanoparticle, and the second DNA strand;
engaging the first emulsion droplet with additional emulsion droplets to achieve the desired valency and
creaming the emulsion forming a floppy network of the plurality of emulsion droplets interconnected by a plurality of patches.

12. The method of claim 11 , wherein the emulsion has a ratio of 5 to 1 of the plurality of linkers to the plurality of droplets, whereby a linear assembly is formed.

13. The method of claim 11 , wherein the emulsion has a ratio of 100 to 1 of the plurality of linkers to the plurality of emulsion droplets, whereby a folded assembly is formed.

14. The method of claim 11 , further comprising selecting a valency for the floppy network.

Assignees

• Univ New York

Inventors

• Brujic Jasna
• Feng Lang
• Pontani Lea-Laetitia
• Chaikin Paul

Classifications

• A61K8/0241

  Containing particulates characterized by their shape and/or structure (see also A61K8/04, A61K8/11, and A61K8/14, further aspects are classified in A61K2800/40 and subcodes) · CPC title

• A23L35/10

  Emulsified foodstuffs · CPC title

• A61Q19/00

  Preparations for care of the skin · CPC title

• A61K8/64 Primary

  Proteins; Peptides; Derivatives or degradation products thereof · CPC title

• A61K2800/413

  Nanosized, i.e. having sizes below 100 nm · CPC title