Biomimetic emulsions

Patent metadata

Field	Value
Publication number	US-9857371-B2
Application number	US-201313889083-A
Country	US
Kind code	B2
Filing date	May 7, 2013
Priority date	May 8, 2012
Publication date	Jan 2, 2018
Grant date	Jan 2, 2018

Abstract

A biomimetic system is provided for use in modeling cell-cell adhesion mechanisms comprising functionalized emulsion droplets. Further, a cell culture medium and a drug delivery system using said biomimetic system are provided.

First claim

What is claimed is:

1. A biomimetic system comprising:
at least two functionalized emulsion droplets, each having an interfacial phospholipid component with an inward facing hydrophobic tail and an outward facing hydrophilic head, a polymer to induce steric repulsion, and a ligand bound to the hydrophilic head that facilitates adhesion;
a surfactant causing electrostatic repulsion,
an aqueous phase of the emulsion comprises an agent with a functional group that binds to the ligand, and
the at least two functionalized emulsion droplets interconnected by the agent bound to the ligand;
wherein the phospholipid component facilitates the formation of at least two emulsion droplets.

2. The system of claim 1 , wherein the ligand is selected from the group of a cadherin and a biotin.

3. The system of claim 1 , wherein the agent comprises a streptavidin.

4. The system of claim 1 , wherein the interfacial phospholipid component comprises an egg phosphatidylcholine.

5. The system of claim 1 , wherein the surfactant comprises a sodium dodecyl sulfate ionic surfactant.

6. The system of claim 1 , wherein the polymer is selected from the group consisting of polyethylene glycol (PEG), polypropylene glycol (PPG), carbohydrate polymer, amino acid polymer or polyvinyl pyrolidone.

7. The system of claim 1 , wherein the polymer and the ligand form a single surface active complex.

8. The system of claim 1 , wherein the agent, once bound to the ligand of one droplet surface, diffuses until it binds to another agent on a neighboring droplet.

9. The system of claim 8 , wherein force is applied to the system in an amount sufficient to reduce the interparticle distance h to be smaller than a critical length hc, wherein below which distance interaction of the surface active agents produces an adhesive state in each of the at least two functionalized emulsion droplets.

10. The system of claim 9 , wherein the ligand is selected from the group of a cadherin and a biotin, the agent is streptavidin, and hc is about 18 nm.

11. The system of claim 9 , wherein the force is applied either via centrifuge or the addition of a salt.

12. The system of claim 1 , further comprising a fluorescent dye to add color to the emulsion.

13. The system of claim 1 , wherein the two functionalized emulsion droplets have a diameter between 4 and 20 microns.

Assignees

• Univ New York

Inventors

• Brujic Jasna
• Pontani Lea-Laetitia

Classifications

• A61K47/557

  the modifying agent being biotin · CPC title

• A61K47/62

  the modifying agent being a protein, peptide or polyamino acid · CPC title

• A61K9/107 Primary

  Emulsions {; Emulsion preconcentrates; Micelles (composition of emulsions A61K47/00)} · CPC title

• G01N33/56966 Primary

  Animal cells · CPC title

• A61K47/24

  containing atoms other than carbon, hydrogen, oxygen, halogen, nitrogen or sulfur, e.g. cyclomethicone or phospholipids · CPC title