Chemiluminescence measurement device and method for same

Patent metadata

Field	Value
Publication number	US-9857308-B2
Application number	US-201415027297-A
Country	US
Kind code	B2
Filing date	Oct 7, 2014
Priority date	Oct 7, 2013
Publication date	Jan 2, 2018
Grant date	Jan 2, 2018

Abstract

The present invention relates to a device and method for measuring chemiluminescence, and an object is to provide a compact and reliable device and method for measuring chemiluminescence. The device includes a vessel group, a nozzle head having a gas suctioning and discharging mechanism, one or more nozzles, which are in communication with the suctioning and discharging mechanism, and to a front end of which a dispensing tip is attachable, and a magnetic force means capable of causing a magnetic field inside the attached dispensing tip, a nozzle moving mechanism, a linkage part provided to the nozzle head, linkable to the reaction vessel and capable of forming a confined space shielded from external light, a linkage part moving mechanism capable of moving the linkage part, a photometer, a shutter, and a reagent injection flow channel capable of injecting a reagent for chemiluminescence.

First claim

The invention claimed is:

1. A device for measuring chemiluminescence, comprising:
a reaction vessel;
a dispensing tip configured to receive a sample containing a substance of interest, a chemiluminescence labeling substance that labels the substance of interest, and magnetic particles to which the substance of interest in the sample is bound;
a magnet configured to apply a magnetic field to the dispensing tip to separate the magnetic particles from the rest of the sample;
a suction and discharge mechanism in communication with the dispensing tip and configured to:
dissociate the labeled substance of interest from the separated magnetic particles, and
dispense the dissociated labeled substance of interest from the dispensing tip into the reaction vessel;

a linkage part configured to be linked to an opening of the reaction vessel to form a confined space inside the linked reaction vessel that is shielded from external light;
a reagent injection flow channel coupled to the linkage part and configured to inject a reagent for producing chemiluminescence into the linked reaction vessel;
a photometer configured to measure an optical state in the linked reaction vessel for a predetermined measuring time after the reagent injection flow channel injects the reagent for producing chemiluminescence into the linked reaction vessel; and
a shutter configured to:
establish a light guiding state between the photometer and the confined space inside the linked reaction vessel, and
switch from the light guiding state to a light shielding state between the photometer and the confined space inside the linked reaction vessel after the predetermined measuring time passes and before release of the linkage part from the linked reaction vessel.

2. The device for measuring chemiluminescence according to claim 1 , further comprising:
a light guiding part configured to guide light between the reaction vessel and the photometer when the shutter is in the light guiding state.

3. The device for measuring chemiluminescence according to claim 2 , further comprising:
one or more additional reaction vessels to which the linkage part is configured to be linked;
one or more additional light guiding parts configured to guide light between the respective additional reaction vessels and the photometer when the shutter is in the light guiding state;
an arrangement body to which respective first ends of the light guiding part and the additional light guiding parts are coupled along a predetermined course; and
a light reception switching mechanism configured to sequentially optically connect the respective first ends of the light guiding part and the additional light guiding parts to the photometer by moving one or both of the arrangement body and the photometer along the predetermined course,
wherein respective second ends of the light guiding part and the additional light guiding parts are in proximity to, or in contact with, the reaction vessel and the additional reaction vessels, respectively.

4. The device for measuring chemiluminescence according to claim 3 , wherein the light reception switching mechanism optically connects the photometer to the first end of the light guiding part whose second end is in proximity to, or in contact with, the linked reaction vessel.

5. The device for measuring chemiluminescence according to claim 3 , further comprising:
a reagent injecting mechanism coupled to the reagent injection flow channel to inject the reagent for producing chemiluminescence through the reagent injection flow channel and into the linked reaction vessel;
wherein before, or during, the injection of the reagent for producing chemiluminescence into the linked reaction vessel, the shutter is configured to establish the light guiding state between the photometer and the confined space inside the linked reaction vessel.

6. The device for measuring chemiluminescence according to claim 2 , further comprising:
a reagent injecting mechanism coupled to the reagent injection flow channel to inject the reagent for producing chemiluminescence through the reagent injection flow channel and into the linked reaction vessel;
wherein before, or during, the injection of the reagent for producing chemiluminescence into the linked reaction vessel, the shutter is configured to establish the light guiding state between the photometer and the confined space inside the linked reaction vessel.

7. The device for measuring chemiluminescence according to claim 1 , further comprising:
a reagent injecting mechanism coupled to the reagent injection flow channel to inject the reagent for producing chemiluminescence through the reagent injection flow channel and into the linked reaction vessel;
wherein before, or during, the injection of the reagent for producing chemiluminescence into the linked reaction vessel, the shutter is configured to establish the light guiding state between the photometer and the confined space inside the linked reaction vessel.

8. A method for measuring chemiluminescence, the method comprising:
receiving, into a dispensing tip, a sample containing a substance of interest, a chemiluminescence labeling substance to label the substance of interest, and magnetic particles to which the substance of interest in the sample is bindable;
labeling the substance of interest with the chemiluminescence labeling substance;
applying a magnetic field to the dispensing tip to separate the magnetic particles from the rest of the sample;
dissociating the labeled substance of interest from the separated magnetic particles;
dispensing the dissociated labeled substance of interest into a reaction vessel;
linking a linkage part to an opening in the reaction vessel to form a confined space inside the linked reaction vessel that is shielded from external light;
injecting a reagent for producing chemiluminescence into the linked reaction vessel from a reagent injection flow channel coupled to the linkage part;
establishing, using a shutter, a light guiding state between a photometer and the confined space inside the linked reaction vessel;
measuring an optical state in the linked reaction vessel, using the photometer, for a predetermined measuring time after injection of the reagent for producing chemiluminescence into the linked reaction vessel; and
switching the shutter from the light guiding state to a light shielding state between the photometer and the confined space inside the linked reaction vessel after the predetermined measuring time passes and before release of the linkage part from the linked reaction vessel.

9. The method for measuring chemiluminescence according to claim 8 ,
wherein the predetermined measuring time depends on one or more factors selected from the group consisting of:
sensitivity of the photometer,
kind of the reagent for producing chemiluminescence,
amount of the reagent for producing chemiluminescence, and
lifetime of the chemiluminescence.

10. The method for measuring chemiluminescence according to claim 9 , further comprising:
sequentially optically connecting, using a light reception switching mechanism, respective first ends of one or more light guiding parts to the photometer, the respective first ends of the light guiding parts being coupled to an arrangement body along a predetermined course, and the light guiding parts having respective second ends in proximity to, or in contact with, the reaction vessel and one or more additional reaction vessels to which the linkage part is configured to be linked;
wherein the light reception switching mechanism moves one or both of the arrangement body and the photometer along the predetermined course to sequentially optically connect the respective f

Assignees

• Universal Bio Research Co Ltd

Inventors

• Tajima Hideji

Classifications

• G01N35/0098

  involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation (magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C) · CPC title

• G01N21/76 Primary

  Chemiluminescence; Bioluminescence · CPC title

• G01N35/1002

  Reagent dispensers · CPC title

• Y10T436/119163

  with aspirator of claimed structure · CPC title

• C12Q1/6816

  characterised by the detection means (C12Q1/6804 takes precedence) · CPC title