Processes of producing fermentation products

Patent metadata

Field	Value
Publication number	US-9856498-B2
Application number	US-201314388595-A
Country	US
Kind code	B2
Filing date	Mar 28, 2013
Priority date	Mar 30, 2012
Publication date	Jan 2, 2018
Grant date	Jan 2, 2018

Abstract

The invention relates to processes of producing a fermentation product, comprising liquefying a starch containing material with an alpha-amylase; pre-saccharifying and/or saccharifying and fermenting using a fermentation organism in the presence of a carbohydrate source generating enzyme and a cellulolytic composition The invention also relates to methods of dewatering whole stillage.

First claim

The invention claimed is:

1. A process of producing a fermentation product, comprising
(a) liquefying a starch-containing material with an alpha-amylase;
(b) saccharifying the liquefied material;
(c) fermenting using a fermentation organism;
wherein a carbohydrate-source generating enzyme and a cellulolytic composition comprising a beta-glucosidase having at least 95% sequence identity to amino acids 20 to 863 of SEQ ID NO: 6 are present or added during the saccharification step (b), fermentation step (c), or a simultaneous saccharification and fermentation, wherein the beta-glucosidase comprises one or more substitutions selected from the group consisting of L89M, G91L, F100D, 1140V, 1186V, S283G, N456E, and F512Y.

2. The process of claim 1 , wherein the hemicellulase content in the cellulolytic composition constitutes less than 10 wt. % or less than 5 wt. % of the cellulolytic composition.

3. The process of claim 1 , wherein the cellulolytic composition further comprises one or more of the following components:
an Aspergillus fumigatus cellobiohydrolase I as shown in SEQ NO: 2 or a variant thereof having at least 95% sequence identity to amino acids 27 to 532 of SEQ ID NO: 2;
(ii) an Aspergillus fumigatus cellobiohydrolase II as shown in SEQ NO: 4 or a variant thereof having at least 95% sequence identity to amino acids 20 to 454 of SEQ ID NO: 4 and
(iii) a Penicillium sp. GH61 polypeptide having cellulolytic enhancing activity as shown in SEQ NO: 8 or a variant thereof having at least 95% sequence identity to amino acids 26 to 253 of SEQ ID NO: 8.

4. The process of claim 1 , wherein the beta-glucosidase comprises a substitution set selected from the group consisting of:
F100D+S283G+N456E+F512Y;
L89M+G91L+I186V+I140V; and
I186V+L89M+G91L+U140V+F100D+S283G+N456E+F512Y, using SEQ ID NO: 6 for numbering.

5. The process of claim 1 , wherein a trehalase is present or added during the saccharification step (b), fermentation step (c), or simultaneous saccharification and fermentation.

6. The process of claim 5 , wherein the trehalase is a Trichoderma trehalase having at least 95% sequence identity to SEQ ID NO: 12.

Assignees

• Novozymes As

Inventors

• Rasmussen Thomas
• Saunders Jeremy
• Croonenberghs James
• Kang Zhengfang
• Craig Joyce
• Akerman Michael John

Classifications

• C12N9/2445

  Beta-glucosidase (3.2.1.21) · CPC title

• C12P7/14 Primary

  Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms · CPC title

• C12P7/06

  Ethanol, i.e. non-beverage · CPC title

• C12P19/14 Primary

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title