Closed system for extracting isolated medium

Patent metadata

Field	Value
Publication number	US-9856450-B2
Application number	US-201514963156-A
Country	US
Kind code	B2
Filing date	Dec 8, 2015
Priority date	Dec 8, 2014
Publication date	Jan 2, 2018
Grant date	Jan 2, 2018

Abstract

An assembly includes an adjustable dip tube that can selectively withdraw an isolated component at various heights within a vessel. The adjustable nature of the dip tube can improve the cell recovery rate as compared to conventional cell recovery assemblies. Additionally, the assembly can take advantage of the adjustable nature of the dip tube while maintaining an aseptic interior cavity of the assembly.

First claim

What is claimed is:

1. An assembly comprising:
a vessel having an interior cavity, the vessel having a closed end and a tube port opposite the closed end, the tube port having a first bore;
an adjustable dip tube configured to be placed within the interior cavity and adapted to withdraw an isolated component in the vessel at different heights with respect to the closed end,
wherein the assembly is adapted to maintain an aseptic, interior cavity as the adjustable dip tube is adjusted to the different heights relative to the closed end,
wherein the assembly comprises a dynamic mechanism comprising the adjustable dip tube and the first bore,
wherein the adjustable dip tube comprises threading, and wherein the first bore comprises threading, and wherein the threading on the adjustable dip tube is adapted to engage with the threading on the first bore, wherein the first bore permits a fluid connection between the interior cavity and an exterior environment outside the adjustable dip tube,
wherein the threading of the first bore extends into the interior cavity, and
wherein the dynamic mechanism is configured to allow the height of the adjustable dip tube to be adjusted with respect to the closed end while withdrawing the isolated component and maintaining the aseptic interior cavity.

2. The assembly of claim 1 , wherein the dip tube includes a first end having a first inner diameter and second end axially opposite the first end including a second inner diameter, wherein the first inner diameter is greater than the second inner diameter.

3. The assembly of claim 1 , wherein the dip tube includes a first end having a first outer diameter and a second end axially opposite the first end including a second outer diameter, wherein the first outer diameter is greater than the second outer diameter.

4. The assembly of claim 1 , wherein the assembly comprises a vent port adapted to selectively vent the interior cavity of the vessel at various heights.

5. The assembly of claim 1 , wherein the dip tube is adapted to be adjustable by a height of at least 0.5 inches.

6. The assembly of claim 1 , wherein the dip tube is fixed within the assembly at a height of at least 0.5 inches.

7. The assembly of claim 1 , wherein the dip tube is adapted to handle biologically active material.

8. The assembly of claim 1 , wherein the assembly is adapted to handle biologically active material.

9. The assembly of claim 1 , wherein the assembly is adapted to reduce the volume of a composition comprising a media and a biologically active substance, and recover the biologically active substance.

10. The assembly of claim 1 , wherein the assembly is adapted for volume reduction and recovery of cells for cell therapy treatments.

Assignees

• Saint Gobain Performance Plastics Corp

Inventors

• Johnson Scott R
• Block Michael S
• Pates Christopher J
• Hallett Avery A

Classifications

• C12M47/04 Primary

  Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04) · CPC title

• C12M33/10

  by centrifugation (centrifuges in general B04B); Cyclones (cyclones in general B04C) · CPC title

• B01L2300/0832

  cylindrical, tube shaped · CPC title

• B01L2300/046

  Function or devices integrated in the closure · CPC title

• B01L2300/042

  Caps; Plugs · CPC title