Bioreactor array and methods of combinatorial testing

Patent metadata

Field	Value
Publication number	US-9856447-B2
Application number	US-201514805267-A
Country	US
Kind code	B2
Filing date	Jul 21, 2015
Priority date	Feb 19, 2013
Publication date	Jan 2, 2018
Grant date	Jan 2, 2018

Abstract

Methods and apparatus for culturing microorganisms are described, including culturing in mixotrophic culture conditions. A bioreactor array with multiple culture vessels with independently controllable inputs is used to culture similar cultures of microorganisms in which at least one parameter differs from other culture vessels in the bioreactor array.

First claim

What is claimed is:

1. A method for conducting combinatorial testing of microorganisms, comprising:
a. providing a plurality of cultures comprising a composition of 0.5-1.5 grams of microalgae capable of using organic carbon as an energy and carbon source in a volume of 500-1,000 ml of an aqueous culture medium and a population of contaminating microorganisms consisting of at least one selected from the group consisting of bacteria, fungi, and ciliates, in a plurality of closed culture vessels comprising lids;
b. independently controlling the supply to each culture vessel of at least one from the group consisting of:
i. light;
ii. at least one gas; and
iii. heat exchange;

c. supplying acetic acid to the plurality of cultures in response to a detected culture pH, wherein the acetic acid provides a source of organic carbon for utilization as energy and carbon by the microalgae and controls the culture pH;
d. venting gas through the lids of the culturing vessels; and
e. testing combinations of different parameters in parallel to determine the effect on the microalgae and the population of contaminating microorganisms, wherein each culture vessel contains a culture of the same composition cultured with different combinations of parameters selected from the group consisting of culture temperature, culture pH, amount of light, intensity of light, wavelengths of light, light photoperiod, light/dark cycle, rate of organic carbon supply, concentrations of gases, and agitation from gas supply.

2. The method of claim 1 , further comprising harvesting at least part of the composition from the culture volume, and wherein the different parameters further comprise harvest rates.

3. The method of claim 1 , further comprising continuously monitoring the parameters of the culture of the microalgae and the population of contaminating microorganisms in the plurality of culture vessels with at least one sensor and a programmable logic controller.

4. The method of claim 1 , wherein the parameters are adjusting during the testing.

5. The method of claim 1 , wherein the testing is conducted in mixotrophic culture conditions.

6. The method of claim 1 , wherein the testing is conducted in heterotrophic culture conditions.

7. The method of claim 1 , further comprising controlling the supply of light by blocking select wavelengths of light with an optical filter.

8. The method of claim 1 , wherein the at least one gas comprises an inhibitory gas selected from the group consisting of ozone, chlorine dioxide, and ethylene oxide, and the inhibitory gas reduces the population of contaminating microorganisms in the culture.

9. The method of claim 2 , wherein the amount of microalgae harvested from a single culture initially comprising 0.5-1.5 grams of the microalgae in 500-1,000 ml of the aqueous culture medium is at least enough biomass to perform composition analytical tests on the microalgae.

10. The method of claim 3 , wherein the step of independently controlling the supply of at least one gas to each culturing vessel further comprises sparging air into each culture vessel and controlling an addition of an inert gas into the sparged air by using a dissolved oxygen sensor and the programmable logic controller.

11. The method of claim 1 , further comprising mixing the cultures with the supply of at least one gas and at least one from the group consisting of a baffle and a separate active mixer.

12. The method of claim 1 , wherein the parameters tested further comprise recycling of the culture medium.

Assignees

• Heliae Dev Llc

Inventors

• Tonkovich Anna Lee
• Adame Thomas
• Qin Shan
• Ganuza Taberna Eneko

Classifications

• C12M41/18

  Heat exchange systems, e.g. heat jackets or outer envelopes · CPC title

• C12M23/22 Primary

  Transparent or translucent parts (glassware for laboratory use B01L3/00) · CPC title

• C12M23/58

  Reaction vessels connected in series or in parallel (combinations of bioreactors with other apparatus, C12M43/00) · CPC title

• C12M41/06

  of illumination · CPC title

• C12M21/02 Primary

  Photobioreactors (culturing algae A01G33/00, A01H4/001, C12N1/12) · CPC title