Recombinant feline leukemia virus vaccine containing optimized feline leukemia virus envelope gene

Patent metadata

Field	Value
Publication number	US-9856295-B2
Application number	US-201414507455-A
Country	US
Kind code	B2
Filing date	Oct 6, 2014
Priority date	Jul 20, 2011
Publication date	Jan 2, 2018
Grant date	Jan 2, 2018

Abstract

The present invention provides vectors that contain and express in vivo or in vitro FeLV antigens that elicit an immune response in animal or human against FeLV, compositions comprising said vectors and/or FeLV polypeptides, methods of vaccination against FeLV, and kits for use with such methods and compositions.

First claim

What we claim is:

1. A composition comprising an expression vector comprising a first polynucleotide encoding an optimized Feline Leukemia Virus (FeLV) envelope (ENV) polypeptide and a second polynucleotide encoding an FeLV GAG/PRO polypeptide, wherein the optimized FeLV ENV polypeptide comprises a mutation at the amino acid corresponding to position 527 of SEQ ID NO:6 or equivalent corresponding amino acid position of an FeLV ENV protein, and wherein the mutation comprises a substitution of arginine (R), aspartic acid (D), or methionine (M) for glutamic acid (E), and wherein the optimized FeLV ENV polypeptide has at least 98% sequence identity to the sequence as set forth in SEQ ID NO: 2.

2. The composition of claim 1 , wherein the mutation comprises a substitution of arginine (R) for glutamic acid (E).

3. The composition of claim 1 , wherein the first polynucleotide has at least 99% sequence identity to the sequence as set forth in SEQ ID NO: 1.

4. The composition of claim 1 , wherein the expression vector is an avipox vector.

5. The composition of claim 1 , wherein the composition is administered to an animal at a dosage range from about 10 5 pfu to about 10 9 pfu.

6. An expression vector comprising a first polynucleotide encoding an optimized FeLV ENV polypeptide and a second polynucleotide encoding an FeLV GAG/PRO polypeptide, wherein the optimized FeLV ENV polypeptide comprises a mutation at the amino acid corresponding to position 527 of SEQ ID NO:6, and wherein the mutation comprises a substitution of arginine (R), aspartic acid (D), or methionine (M) for glutamic acid (E), and wherein the optimized FeLV ENV polypeptide has at least 98% sequence identity to the sequence as set forth in SEQ ID NO: 2.

7. The expression vector of claim 6 , wherein the mutation comprises a substitution of arginine (R) for glutamic acid (E).

8. The expression vector of claim 6 , wherein the first polynucleotide has at least 99% sequence identity to the sequence as set forth in SEQ ID NO: 1.

9. The expression vector of claim 6 , wherein the expression vector is an avipox vector.

10. The expression vector of claim 6 , wherein the composition is administered to an animal at a dosage range from about 10 5 pfu to about 10 9 pfu.

11. A method of vaccinating an animal comprising at least one administration of the composition or expression vector of claim 1 or 6 .

12. The method of claim 11 , wherein the method comprises a prime-boost administration regime.

13. The method of claim 11 , wherein the composition is administered at a dosage range from about 10 5 pfu to about 10 9 pfu.

14. The method of claim 12 , wherein the composition is administered at a dosage range from about 10 5 pfu to about 10 9 pfu.

Assignees

• Merial Ltd
• Centre Nat Rech Scient
• Roussy Inst Gustave
• Univ Paris-Sud
• Merial Inc

Inventors

• Poulet Herve
• Heidmann Thierry

Classifications

• A61P37/04

  Immunostimulants · CPC title

• A61P31/14

  for RNA viruses · CPC title

• A61P35/02

  specific for leukemia · CPC title

• A61K39/21

  Retroviridae, e.g. equine infectious anemia virus · CPC title

• A61K39/275

  Poxviridae, e.g. avipoxvirus · CPC title